Determination of Storage (Starch/Glycogen) and Total Saccharides Content in Algae and Cyanobacteria by a Phenol-Sulfuric Acid Method.

Zavřel, T; Očenášová, P; Sinetova, MA; Červený, J

Determination of Storage (Starch/Glycogen) and Total Saccharides Content in Algae and Cyanobacteria by a Phenol-Sulfuric Acid Method.

Zavřel, T Očenášová, P Sinetova, MA Červený, J

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Publisher:: Bio-Protocol, LLC
Publication Type:: Journal Article
Citation:: Bio Protoc, 2018, 8, (15), pp. e2966
Issue Date:: 2018-08-05

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Field	Value	Language
dc.contributor.author	Zavřel, T
dc.contributor.author	Očenášová, P
dc.contributor.author	Sinetova, MA
dc.contributor.author	Červený, J
dc.date.accessioned	2022-09-11T21:50:46Z
dc.date.available	2018-07-28
dc.date.available	2022-09-11T21:50:46Z
dc.date.issued	2018-08-05
dc.identifier.citation	Bio Protoc, 2018, 8, (15), pp. e2966
dc.identifier.issn	2331-8325
dc.identifier.issn	2331-8325
dc.identifier.uri	http://hdl.handle.net/10453/161699
dc.description.abstract	This is a protocol for quantitative determination of storage and total carbohydrates in algae and cyanobacteria. The protocol is simple, fast and sensitive and it requires only few standard chemicals. Great advantage of this protocol is that both storage and total saccharides can be determined in the cellular pellets that were already used for chlorophyll and carotenoids quantification. Since it is recommended to perform the pigments measurement in triplicates, each pigment analysis can generate samples for both total saccharide and glycogen/starch content quantification. The protocol was applied for quantification of both storage and total carbohydrates in cyanobacteria Synechocystis sp. PCC 6803, Cyanothece sp. ATCC 51142 and Cyanobacterium sp. IPPAS B-1200. It was also applied for estimation of storage polysaccharides in Galdieria (IPPAS P-500, IPPAS P-507, IPPAS P-508, IPPAS P-513), Cyanidium caldarium IPPAS P-510, in green algae Chlorella sp. IPPAS C-1 and C-1210, Parachlorella kessleri IPPAS C-9, Nannochloris sp. C-1509, Coelastrella sp. IPPAS H-626, Haematococcus sp. IPPAS H-629 and H-239, and in Eustigmatos sp. IPPAS H-242 and IPPAS C-70.
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	Bio-Protocol, LLC
dc.relation.ispartof	Bio Protoc
dc.relation.isbasedon	10.21769/BioProtoc.2966
dc.rights	info:eu-repo/semantics/closedAccess
dc.title	Determination of Storage (Starch/Glycogen) and Total Saccharides Content in Algae and Cyanobacteria by a Phenol-Sulfuric Acid Method.
dc.type	Journal Article
utslib.citation.volume	8
utslib.location.activity	United States
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access	*
dc.date.updated	2022-09-11T21:50:44Z
pubs.issue	15
pubs.publication-status	Published online
pubs.volume	8
utslib.citation.issue	15

Abstract:

This is a protocol for quantitative determination of storage and total carbohydrates in algae and cyanobacteria. The protocol is simple, fast and sensitive and it requires only few standard chemicals. Great advantage of this protocol is that both storage and total saccharides can be determined in the cellular pellets that were already used for chlorophyll and carotenoids quantification. Since it is recommended to perform the pigments measurement in triplicates, each pigment analysis can generate samples for both total saccharide and glycogen/starch content quantification. The protocol was applied for quantification of both storage and total carbohydrates in cyanobacteria Synechocystis sp. PCC 6803, Cyanothece sp. ATCC 51142 and Cyanobacterium sp. IPPAS B-1200. It was also applied for estimation of storage polysaccharides in Galdieria (IPPAS P-500, IPPAS P-507, IPPAS P-508, IPPAS P-513), Cyanidium caldarium IPPAS P-510, in green algae Chlorella sp. IPPAS C-1 and C-1210, Parachlorella kessleri IPPAS C-9, Nannochloris sp. C-1509, Coelastrella sp. IPPAS H-626, Haematococcus sp. IPPAS H-629 and H-239, and in Eustigmatos sp. IPPAS H-242 and IPPAS C-70.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/161699