Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood.

Deng, F; Li, Y; Hossain, MJ; Kendig, MD; Arnold, R; Goldys, EM; Morris, MJ; Liu, G

Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood.

Deng, F Li, Y Hossain, MJ Kendig, MD Arnold, R Goldys, EM Morris, MJ Liu, G

Permalink

Publisher:: ROYAL SOC CHEMISTRY
Publication Type:: Journal Article
Citation:: Analyst, 2019, 144, (19), pp. 5682-5690
Issue Date:: 2019-10-07

Closed Access

	Filename	Description	Size
	c9an01300h.pdf	Published version	3.67 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Deng, F
dc.contributor.author	Li, Y
dc.contributor.author	Hossain, MJ
dc.contributor.author	Kendig, MD
dc.contributor.author	Arnold, R
dc.contributor.author	Goldys, EM
dc.contributor.author	Morris, MJ
dc.contributor.author	Liu, G
dc.date.accessioned	2022-09-11T22:09:54Z
dc.date.available	2022-09-11T22:09:54Z
dc.date.issued	2019-10-07
dc.identifier.citation	Analyst, 2019, 144, (19), pp. 5682-5690
dc.identifier.issn	0003-2654
dc.identifier.issn	1364-5528
dc.identifier.uri	http://hdl.handle.net/10453/161709
dc.description.abstract	A sandwich immunosensor was successfully developed for monitoring of interleukin-1β (IL-1β) in rat whole blood. The substrate stainless steel (SS) was first coated with a polydopamine layer and subsequently grafted with poly(ethylene glycol) methacrylate brushes, onto which a sandwich immunosensor was modified for detection of IL-1β. The device has been successfully applied for monitoring of IL-1β with a limit of detection of 4.7 pg mL-1, and a linear detection range of 12.5-200 pg mL-1. Good specificity and selectivity for monitoring of IL-1β in rat macrophage secretion were achieved. Furthermore, this device was validated by detection of IL-1β in rat whole blood samples with greater concentrations observed in obese rats compared to control, and strong positive correlation between concentrations of IL-1β and blood glucose. These results suggest this device is feasible for direct detection of target analytes in biological samples.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ROYAL SOC CHEMISTRY
dc.relation	http://purl.org/au-research/grants/arc/CE140100003
dc.relation.ispartof	Analyst
dc.relation.isbasedon	10.1039/c9an01300h
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry, 0399 Other Chemical Sciences
dc.subject.classification	Analytical Chemistry
dc.subject.mesh	Animals
dc.subject.mesh	Antibodies, Immobilized
dc.subject.mesh	Antibodies, Monoclonal, Murine-Derived
dc.subject.mesh	Equidae
dc.subject.mesh	Fluorescence
dc.subject.mesh	Fluorescent Dyes
dc.subject.mesh	Goats
dc.subject.mesh	Immunoassay
dc.subject.mesh	Indoles
dc.subject.mesh	Interleukin-1beta
dc.subject.mesh	Limit of Detection
dc.subject.mesh	Methacrylates
dc.subject.mesh	Mice
dc.subject.mesh	Oxazines
dc.subject.mesh	Polyethylene Glycols
dc.subject.mesh	Polymers
dc.subject.mesh	Polystyrenes
dc.subject.mesh	Rats
dc.subject.mesh	Reproducibility of Results
dc.subject.mesh	Stainless Steel
dc.subject.mesh	Animals
dc.subject.mesh	Goats
dc.subject.mesh	Equidae
dc.subject.mesh	Mice
dc.subject.mesh	Rats
dc.subject.mesh	Stainless Steel
dc.subject.mesh	Polyethylene Glycols
dc.subject.mesh	Methacrylates
dc.subject.mesh	Polystyrenes
dc.subject.mesh	Oxazines
dc.subject.mesh	Indoles
dc.subject.mesh	Polymers
dc.subject.mesh	Fluorescent Dyes
dc.subject.mesh	Immunoassay
dc.subject.mesh	Reproducibility of Results
dc.subject.mesh	Fluorescence
dc.subject.mesh	Interleukin-1beta
dc.subject.mesh	Antibodies, Immobilized
dc.subject.mesh	Limit of Detection
dc.subject.mesh	Antibodies, Monoclonal, Murine-Derived
dc.title	Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood.
dc.type	Journal Article
utslib.citation.volume	144
utslib.location.activity	England
utslib.for	0301 Analytical Chemistry
utslib.for	0399 Other Chemical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2022-09-11T22:09:17Z
pubs.issue	19
pubs.publication-status	Published
pubs.volume	144
utslib.citation.issue	19

Abstract:

A sandwich immunosensor was successfully developed for monitoring of interleukin-1β (IL-1β) in rat whole blood. The substrate stainless steel (SS) was first coated with a polydopamine layer and subsequently grafted with poly(ethylene glycol) methacrylate brushes, onto which a sandwich immunosensor was modified for detection of IL-1β. The device has been successfully applied for monitoring of IL-1β with a limit of detection of 4.7 pg mL-1, and a linear detection range of 12.5-200 pg mL-1. Good specificity and selectivity for monitoring of IL-1β in rat macrophage secretion were achieved. Furthermore, this device was validated by detection of IL-1β in rat whole blood samples with greater concentrations observed in obese rats compared to control, and strong positive correlation between concentrations of IL-1β and blood glucose. These results suggest this device is feasible for direct detection of target analytes in biological samples.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/161709