Molecular Identification of Gambierdiscus and Fukuyoa (Dinophyceae) from Environmental Samples.
- Publisher:
- MDPI AG
- Publication Type:
- Journal Article
- Citation:
- Marine Drugs, 2017, 15, (8), pp. 1-13
- Issue Date:
- 2017-08-02
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Smith, KF | |
dc.contributor.author | Biessy, L | |
dc.contributor.author | Argyle, PA | |
dc.contributor.author | Trnski, T | |
dc.contributor.author | Halafihi, T | |
dc.contributor.author | Rhodes, LL | |
dc.date.accessioned | 2022-09-13T01:50:20Z | |
dc.date.available | 2017-07-28 | |
dc.date.available | 2022-09-13T01:50:20Z | |
dc.date.issued | 2017-08-02 | |
dc.identifier.citation | Marine Drugs, 2017, 15, (8), pp. 1-13 | |
dc.identifier.issn | 1660-3397 | |
dc.identifier.issn | 1660-3397 | |
dc.identifier.uri | http://hdl.handle.net/10453/161774 | |
dc.description.abstract | Ciguatera Fish Poisoning (CFP) is increasing across the Pacific and the distribution of the causative dinoflagellates appears to be expanding. Subtle differences in thecal plate morphology are used to distinguish dinoflagellate species, which are difficult to determine using light microscopy. For these reasons we sought to develop a Quantitative PCR assay that would detect all species from both Gambierdiscus and Fukuyoa genera in order to rapidly screen environmental samples for potentially toxic species. Additionally, a specific assay for F. paulensis was developed as this species is of concern in New Zealand coastal waters. Using the assays we analyzed 31 samples from three locations around New Zealand and the Kingdom of Tonga. Fourteen samples in total were positive for Gambierdiscus/Fukuyoa and two samples were also positive using the F. paulensis assay. Samples from the Kermadec Islands were further characterized using high-throughput sequencing metabarcoding. The majority of reads corresponded to Gambierdiscus species with three species identified at all sites (G. australes, G. honu and G. polynesiensis). This is the first confirmed identification of G. polynesiensis, a known ciguatoxin producer, in New Zealand waters. Other known toxin-producing genera were also detected, included Alexandrium, Amphidinium, Azadinium, Dinophysis, Ostreopsis, and Prorocentrum. | |
dc.format | Electronic | |
dc.language | eng | |
dc.publisher | MDPI AG | |
dc.relation.ispartof | Marine Drugs | |
dc.relation.isbasedon | 10.3390/md15080243 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | 0306 Physical Chemistry (incl. Structural), 1115 Pharmacology and Pharmaceutical Sciences | |
dc.subject.classification | Medicinal & Biomolecular Chemistry | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Biological Assay | |
dc.subject.mesh | Ciguatera Poisoning | |
dc.subject.mesh | Dinoflagellida | |
dc.subject.mesh | Environment | |
dc.subject.mesh | Marine Toxins | |
dc.subject.mesh | New Zealand | |
dc.subject.mesh | Oceans and Seas | |
dc.subject.mesh | Phylogeny | |
dc.subject.mesh | Polymerase Chain Reaction | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Biological Assay | |
dc.subject.mesh | Ciguatera Poisoning | |
dc.subject.mesh | Dinoflagellida | |
dc.subject.mesh | Environment | |
dc.subject.mesh | Marine Toxins | |
dc.subject.mesh | New Zealand | |
dc.subject.mesh | Oceans and Seas | |
dc.subject.mesh | Phylogeny | |
dc.subject.mesh | Polymerase Chain Reaction | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Dinoflagellida | |
dc.subject.mesh | Ciguatera Poisoning | |
dc.subject.mesh | Marine Toxins | |
dc.subject.mesh | Biological Assay | |
dc.subject.mesh | Polymerase Chain Reaction | |
dc.subject.mesh | Environment | |
dc.subject.mesh | Phylogeny | |
dc.subject.mesh | New Zealand | |
dc.subject.mesh | Oceans and Seas | |
dc.title | Molecular Identification of Gambierdiscus and Fukuyoa (Dinophyceae) from Environmental Samples. | |
dc.type | Journal Article | |
utslib.citation.volume | 15 | |
utslib.location.activity | Switzerland | |
utslib.for | 0306 Physical Chemistry (incl. Structural) | |
utslib.for | 1115 Pharmacology and Pharmaceutical Sciences | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Strength - C3 - Climate Change Cluster | |
utslib.copyright.status | open_access | * |
pubs.consider-herdc | false | |
dc.date.updated | 2022-09-13T01:50:15Z | |
pubs.issue | 8 | |
pubs.publication-status | Published | |
pubs.volume | 15 | |
utslib.citation.issue | 8 |
Abstract:
Ciguatera Fish Poisoning (CFP) is increasing across the Pacific and the distribution of the causative dinoflagellates appears to be expanding. Subtle differences in thecal plate morphology are used to distinguish dinoflagellate species, which are difficult to determine using light microscopy. For these reasons we sought to develop a Quantitative PCR assay that would detect all species from both Gambierdiscus and Fukuyoa genera in order to rapidly screen environmental samples for potentially toxic species. Additionally, a specific assay for F. paulensis was developed as this species is of concern in New Zealand coastal waters. Using the assays we analyzed 31 samples from three locations around New Zealand and the Kingdom of Tonga. Fourteen samples in total were positive for Gambierdiscus/Fukuyoa and two samples were also positive using the F. paulensis assay. Samples from the Kermadec Islands were further characterized using high-throughput sequencing metabarcoding. The majority of reads corresponded to Gambierdiscus species with three species identified at all sites (G. australes, G. honu and G. polynesiensis). This is the first confirmed identification of G. polynesiensis, a known ciguatoxin producer, in New Zealand waters. Other known toxin-producing genera were also detected, included Alexandrium, Amphidinium, Azadinium, Dinophysis, Ostreopsis, and Prorocentrum.
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