Insights into the interfacial structure-function of poly(ethylene glycol)-decorated peptide-stabilised nanoscale emulsions.

Tayeb, HH; Piantavigna, S; Howard, CB; Nouwens, A; Mahler, SM; Middelberg, APJ; He, L; Holt, SA; Sainsbury, F

Insights into the interfacial structure-function of poly(ethylene glycol)-decorated peptide-stabilised nanoscale emulsions.

Tayeb, HH Piantavigna, S Howard, CB Nouwens, A Mahler, SM Middelberg, APJ He, L Holt, SA Sainsbury, F

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Publisher:: ROYAL SOC CHEMISTRY
Publication Type:: Journal Article
Citation:: Soft Matter, 2017, 13, (43), pp. 7953-7961
Issue Date:: 2017-11-08

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Field	Value	Language
dc.contributor.author	Tayeb, HH
dc.contributor.author	Piantavigna, S
dc.contributor.author	Howard, CB
dc.contributor.author	Nouwens, A
dc.contributor.author	Mahler, SM
dc.contributor.author	Middelberg, APJ
dc.contributor.author	He, L
dc.contributor.author	Holt, SA
dc.contributor.author	Sainsbury, F
dc.date.accessioned	2022-10-03T05:31:02Z
dc.date.available	2022-10-03T05:31:02Z
dc.date.issued	2017-11-08
dc.identifier.citation	Soft Matter, 2017, 13, (43), pp. 7953-7961
dc.identifier.issn	1744-683X
dc.identifier.issn	1744-6848
dc.identifier.uri	http://hdl.handle.net/10453/162231
dc.description.abstract	The interfacial properties of nanoscale materials have profound influence on biodistribution and stability as well as the effectiveness of sophisticated surface-encoded properties such as active targeting to cell surface receptors. Tailorable nanocarrier emulsions (TNEs) are a novel class of oil-in-water emulsions stabilised by molecularly-engineered biosurfactants that permit single-pot stepwise surface modification with related polypeptides that may be chemically conjugated or genetically fused to biofunctional moieties. We have probed the structure and function of poly(ethylene glycol) (PEG) used to decorate TNEs in this way. The molecular weight of PEG decorating TNEs has considerable impact on the ζ-potential of the emulsion particles, related to differential interfacial thickness of the PEG layer as determined by X-ray reflectometry. By co-modifying TNEs with an antibody fragment, we show that the molecular weight and density of PEG governs the competing parameters of accessibility of the targeting moiety and of shielding the interface from non-specific interactions with the environment. The fundamental understanding of the molecular details of the PEG layer that we present provides valuable insights into the structure-function relationship for soft nanomaterial interfaces. This work therefore paves the way for further rational design of TNEs and other nanocarriers that must interact with their environment in controlled and predictable ways.
dc.format	Print
dc.language	eng
dc.publisher	ROYAL SOC CHEMISTRY
dc.relation.ispartof	Soft Matter
dc.relation.isbasedon	10.1039/c7sm01614j
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	02 Physical Sciences, 03 Chemical Sciences, 09 Engineering
dc.subject.classification	Chemical Physics
dc.title	Insights into the interfacial structure-function of poly(ethylene glycol)-decorated peptide-stabilised nanoscale emulsions.
dc.type	Journal Article
utslib.citation.volume	13
utslib.location.activity	England
utslib.for	02 Physical Sciences
utslib.for	03 Chemical Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2022-10-03T05:30:59Z
pubs.issue	43
pubs.publication-status	Published
pubs.volume	13
utslib.citation.issue	43

Abstract:

The interfacial properties of nanoscale materials have profound influence on biodistribution and stability as well as the effectiveness of sophisticated surface-encoded properties such as active targeting to cell surface receptors. Tailorable nanocarrier emulsions (TNEs) are a novel class of oil-in-water emulsions stabilised by molecularly-engineered biosurfactants that permit single-pot stepwise surface modification with related polypeptides that may be chemically conjugated or genetically fused to biofunctional moieties. We have probed the structure and function of poly(ethylene glycol) (PEG) used to decorate TNEs in this way. The molecular weight of PEG decorating TNEs has considerable impact on the ζ-potential of the emulsion particles, related to differential interfacial thickness of the PEG layer as determined by X-ray reflectometry. By co-modifying TNEs with an antibody fragment, we show that the molecular weight and density of PEG governs the competing parameters of accessibility of the targeting moiety and of shielding the interface from non-specific interactions with the environment. The fundamental understanding of the molecular details of the PEG layer that we present provides valuable insights into the structure-function relationship for soft nanomaterial interfaces. This work therefore paves the way for further rational design of TNEs and other nanocarriers that must interact with their environment in controlled and predictable ways.

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http://hdl.handle.net/10453/162231