Efficient Enzymatic Oxidation of Glucose Mediated by Ferrocene Covalently Attached to Polyethylenimine Stabilized Gold Nanoparticles

Dilusha Cooray, MC; Sandanayake, S; Li, F; Langford, SJ; Bond, AM; Zhang, J

Efficient Enzymatic Oxidation of Glucose Mediated by Ferrocene Covalently Attached to Polyethylenimine Stabilized Gold Nanoparticles

Dilusha Cooray, MC Sandanayake, S Li, F Langford, SJ Bond, AM Zhang, J

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Publisher:: WILEY-V C H VERLAG GMBH
Publication Type:: Journal Article
Citation:: Electroanalysis, 2016, 28, (11), pp. 2728-2736
Issue Date:: 2016-11-01

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Field	Value	Language
dc.contributor.author	Dilusha Cooray, MC
dc.contributor.author	Sandanayake, S
dc.contributor.author	Li, F
dc.contributor.author	Langford, SJ
dc.contributor.author	Bond, AM
dc.contributor.author	Zhang, J
dc.date.accessioned	2022-10-24T05:26:01Z
dc.date.available	2022-10-24T05:26:01Z
dc.date.issued	2016-11-01
dc.identifier.citation	Electroanalysis, 2016, 28, (11), pp. 2728-2736
dc.identifier.issn	1040-0397
dc.identifier.issn	1521-4109
dc.identifier.uri	http://hdl.handle.net/10453/162635
dc.description.abstract	Bioanodes for fuel cell applications require highly efficient oxidation reactions to achieve a sufficiently large current density. In this study, gold nanoparticles have been synthesized using branched polyethylenimine (bPEI), a well-known polymer that forms a hydrogel in water, as the stabilizer. Primary amine groups available in bPEI provide active sites for further conjugation with ferrocene propionic acid via the 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide coupling reaction, with the enzyme glucose oxidase, using glutaraldehyde as linkers. This composite material was then used for the fabrication of glucose oxidase electrodes by drop casting of aqueous solutions onto glassy carbon electrodes. The three-dimensional structure offered by the new hydrogel facilitates communication between the enzyme and the electrode through the redox mediator ferrocene. This allows the glucose oxidase electrode to exhibit excellent activity towards electrocatalytic oxidation of glucose in phosphate buffer solutions at pH 7 with a maximum current density of approximately 800 μA cm−2, one of the highest values reported so far for redox hydrogel based glucose oxidase electrodes. Over a wide glucose concentration range, the enzyme response follows that predicted by the Michaelis-Menten equation with a Michaelis constant of 8.4 mM. In the sensing context, this electrode also exhibits a wide linear dynamic glucose concentration range of 0.5–10 mM with a limit of detection of 0.04 mM.
dc.language	English
dc.publisher	WILEY-V C H VERLAG GMBH
dc.relation.ispartof	Electroanalysis
dc.relation.isbasedon	10.1002/elan.201600201
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry, 0306 Physical Chemistry (incl. Structural), 0399 Other Chemical Sciences
dc.subject.classification	Analytical Chemistry
dc.title	Efficient Enzymatic Oxidation of Glucose Mediated by Ferrocene Covalently Attached to Polyethylenimine Stabilized Gold Nanoparticles
dc.type	Journal Article
utslib.citation.volume	28
utslib.for	0301 Analytical Chemistry
utslib.for	0306 Physical Chemistry (incl. Structural)
utslib.for	0399 Other Chemical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CCET - Centre for Clean Energy Technology
utslib.copyright.status	closed_access	*
dc.date.updated	2022-10-24T05:26:00Z
pubs.issue	11
pubs.publication-status	Published
pubs.volume	28
utslib.citation.issue	11

Abstract:

Bioanodes for fuel cell applications require highly efficient oxidation reactions to achieve a sufficiently large current density. In this study, gold nanoparticles have been synthesized using branched polyethylenimine (bPEI), a well-known polymer that forms a hydrogel in water, as the stabilizer. Primary amine groups available in bPEI provide active sites for further conjugation with ferrocene propionic acid via the 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide coupling reaction, with the enzyme glucose oxidase, using glutaraldehyde as linkers. This composite material was then used for the fabrication of glucose oxidase electrodes by drop casting of aqueous solutions onto glassy carbon electrodes. The three-dimensional structure offered by the new hydrogel facilitates communication between the enzyme and the electrode through the redox mediator ferrocene. This allows the glucose oxidase electrode to exhibit excellent activity towards electrocatalytic oxidation of glucose in phosphate buffer solutions at pH 7 with a maximum current density of approximately 800 μA cm−2, one of the highest values reported so far for redox hydrogel based glucose oxidase electrodes. Over a wide glucose concentration range, the enzyme response follows that predicted by the Michaelis-Menten equation with a Michaelis constant of 8.4 mM. In the sensing context, this electrode also exhibits a wide linear dynamic glucose concentration range of 0.5–10 mM with a limit of detection of 0.04 mM.

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http://hdl.handle.net/10453/162635