Anti-Influenza Virus Potential of Probiotic Strain Lactoplantibacillus plantarum YML015 Isolated from Korean Fermented Vegetable

Majumder, R; Alam, MB; Paudel, KR; Ahmed, KA; Devkota, HP; Lee, S-H; Hansbro, PM; Park, Y-H

Anti-Influenza Virus Potential of Probiotic Strain Lactoplantibacillus plantarum YML015 Isolated from Korean Fermented Vegetable

Majumder, R Alam, MB Paudel, KR Ahmed, KA Devkota, HP Lee, S-H Hansbro, PM Park, Y-H

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Publisher:: MDPI AG
Publication Type:: Journal Article
Citation:: Fermentation, 8, (11), pp. 572-572

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Field	Value	Language
dc.contributor.author	Majumder, R
dc.contributor.author	Alam, MB
dc.contributor.author	Paudel, KR
dc.contributor.author	Ahmed, KA
dc.contributor.author	Devkota, HP
dc.contributor.author	Lee, S-H
dc.contributor.author	Hansbro, PM
dc.contributor.author	Park, Y-H
dc.date.accessioned	2022-10-24T17:42:51Z
dc.date.available	2022-10-24T17:42:51Z
dc.identifier.citation	Fermentation, 8, (11), pp. 572-572
dc.identifier.issn	2311-5637
dc.identifier.uri	http://hdl.handle.net/10453/162643
dc.description.abstract	<jats:p>Lactic acid bacteria are one of the potential natural remedies used worldwide, commonly known as probiotics. Here, the aim of this research investigation was to isolate a probiotic Lactobacilli strain, YLM015, from the popular Korean fermented vegetable “Kimchi” and to evaluate its anti-viral potential against influenza virus A (IFVA) H1N1 using the MDCK cell line in vitro, and in embryonated eggs in ovo. The YML015 strain was selected from among the 1200 Lactobacilli isolates for further studies based on its potent anti-viral efficacy. YML015 was identified and characterized as Lactoplantibacillus plantarum YML015 based on the 16S rRNA gene sequencing and biochemically with an API 50 CHL Kit. In ovo assay experienced with embryonated eggs and the hemagglutination inhibition method, as well as cytopathogenic reduction assay, was performed individually to observe anti-influenza viral activity of YML015 against influenza virus A H1N1. Additionally, YML015 was classified for its non-resistance nature as safe for humans and animals as confirmed by the antibiotic susceptibility (MIC) test, cell viability, and hemolysis assay. The heat stability test was also experienced by using different heat-treated cell-free supernatant (CFS) samples of YML015. As a result, YML015 showed highly potent anti-viral activity against influenza virus A H1N1 in vitro in the MDCK cell line. Overall findings suggest that anti-influenza viral activity of L. plantarum YML015 makes it a potential candidate of choice for use as an influential probiotic in pharmacological preparations to protect humans and animals from flu and viral infection.</jats:p>
dc.language	en
dc.publisher	MDPI AG
dc.relation.ispartof	Fermentation
dc.relation.isbasedon	10.3390/fermentation8110572
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Anti-Influenza Virus Potential of Probiotic Strain Lactoplantibacillus plantarum YML015 Isolated from Korean Fermented Vegetable
dc.type	Journal Article
utslib.citation.volume	8
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CFI - Centre for Inflammation
utslib.copyright.status	open_access	*
dc.date.updated	2022-10-24T17:42:50Z
pubs.issue	11
pubs.publication-status	Published online
pubs.volume	8
utslib.citation.issue	11

Abstract:

Lactic acid bacteria are one of the potential natural remedies used worldwide, commonly known as probiotics. Here, the aim of this research investigation was to isolate a probiotic Lactobacilli strain, YLM015, from the popular Korean fermented vegetable “Kimchi” and to evaluate its anti-viral potential against influenza virus A (IFVA) H1N1 using the MDCK cell line in vitro, and in embryonated eggs in ovo. The YML015 strain was selected from among the 1200 Lactobacilli isolates for further studies based on its potent anti-viral efficacy. YML015 was identified and characterized as Lactoplantibacillus plantarum YML015 based on the 16S rRNA gene sequencing and biochemically with an API 50 CHL Kit. In ovo assay experienced with embryonated eggs and the hemagglutination inhibition method, as well as cytopathogenic reduction assay, was performed individually to observe anti-influenza viral activity of YML015 against influenza virus A H1N1. Additionally, YML015 was classified for its non-resistance nature as safe for humans and animals as confirmed by the antibiotic susceptibility (MIC) test, cell viability, and hemolysis assay. The heat stability test was also experienced by using different heat-treated cell-free supernatant (CFS) samples of YML015. As a result, YML015 showed highly potent anti-viral activity against influenza virus A H1N1 in vitro in the MDCK cell line. Overall findings suggest that anti-influenza viral activity of L. plantarum YML015 makes it a potential candidate of choice for use as an influential probiotic in pharmacological preparations to protect humans and animals from flu and viral infection.

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http://hdl.handle.net/10453/162643