Chemotaxis may assist marine heterotrophic bacterial diazotrophs to find microzones suitable for N2 fixation in the pelagic ocean.

Hallstrøm, S; Raina, J-B; Ostrowski, M; Parks, DH; Tyson, GW; Hugenholtz, P; Stocker, R; Seymour, JR; Riemann, L

Chemotaxis may assist marine heterotrophic bacterial diazotrophs to find microzones suitable for N2 fixation in the pelagic ocean.

Hallstrøm, S Raina, J-B Ostrowski, M

Parks, DH Tyson, GW Hugenholtz, P Stocker, R Seymour, JR Riemann, L

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Publisher:: SPRINGERNATURE
Publication Type:: Journal Article
Citation:: ISME J, 2022, 16, (11), pp. 2525-2534
Issue Date:: 2022-11

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Field	Value	Language
dc.contributor.author	Hallstrøm, S
dc.contributor.author	Raina, J-B
dc.contributor.author	Ostrowski, M https://orcid.org/0000-0002-4357-3023
dc.contributor.author	Parks, DH
dc.contributor.author	Tyson, GW
dc.contributor.author	Hugenholtz, P
dc.contributor.author	Stocker, R
dc.contributor.author	Seymour, JR
dc.contributor.author	Riemann, L
dc.date.accessioned	2022-11-03T07:26:48Z
dc.date.available	2022-07-20
dc.date.available	2022-11-03T07:26:48Z
dc.date.issued	2022-11
dc.identifier.citation	ISME J, 2022, 16, (11), pp. 2525-2534
dc.identifier.issn	1751-7362
dc.identifier.issn	1751-7370
dc.identifier.uri	http://hdl.handle.net/10453/163211
dc.description.abstract	Heterotrophic bacterial diazotrophs (HBDs) are ubiquitous in the pelagic ocean, where they have been predicted to carry out the anaerobic process of nitrogen fixation within low-oxygen microenvironments associated with marine pelagic particles. However, the mechanisms enabling particle colonization by HBDs are unknown. We hypothesized that HBDs use chemotaxis to locate and colonize suitable microenvironments, and showed that a cultivated marine HBD is chemotactic toward amino acids and phytoplankton-derived DOM. Using an in situ chemotaxis assay, we also discovered that diverse HBDs at a coastal site are motile and chemotactic toward DOM from various phytoplankton taxa and, indeed, that the proportion of diazotrophs was up to seven times higher among the motile fraction of the bacterial community compared to the bulk seawater community. Finally, three of four HBD isolates and 16 of 17 HBD metagenome assembled genomes, recovered from major ocean basins and locations along the Australian coast, each encoded >85% of proteins affiliated with the bacterial chemotaxis pathway. These results document the widespread capacity for chemotaxis in diverse and globally relevant marine HBDs. We suggest that HBDs could use chemotaxis to seek out and colonize low-oxygen microenvironments suitable for nitrogen fixation, such as those formed on marine particles. Chemotaxis in HBDs could therefore affect marine nitrogen and carbon biogeochemistry by facilitating nitrogen fixation within otherwise oxic waters, while also altering particle degradation and the efficiency of the biological pump.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	SPRINGERNATURE
dc.relation.ispartof	ISME J
dc.relation.isbasedon	10.1038/s41396-022-01299-4
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	05 Environmental Sciences, 06 Biological Sciences, 10 Technology
dc.subject.classification	Microbiology
dc.subject.mesh	Amino Acids
dc.subject.mesh	Australia
dc.subject.mesh	Carbon
dc.subject.mesh	Chemotaxis
dc.subject.mesh	Cyanobacteria
dc.subject.mesh	Membrane Transport Proteins
dc.subject.mesh	Nitrogen
dc.subject.mesh	Nitrogen Fixation
dc.subject.mesh	Oceans and Seas
dc.subject.mesh	Oxygen
dc.subject.mesh	Phytoplankton
dc.subject.mesh	Seawater
dc.subject.mesh	Phytoplankton
dc.subject.mesh	Cyanobacteria
dc.subject.mesh	Carbon
dc.subject.mesh	Oxygen
dc.subject.mesh	Nitrogen
dc.subject.mesh	Amino Acids
dc.subject.mesh	Membrane Transport Proteins
dc.subject.mesh	Seawater
dc.subject.mesh	Nitrogen Fixation
dc.subject.mesh	Chemotaxis
dc.subject.mesh	Australia
dc.subject.mesh	Oceans and Seas
dc.title	Chemotaxis may assist marine heterotrophic bacterial diazotrophs to find microzones suitable for N2 fixation in the pelagic ocean.
dc.type	Journal Article
utslib.citation.volume	16
utslib.location.activity	England
utslib.for	05 Environmental Sciences
utslib.for	06 Biological Sciences
utslib.for	10 Technology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access	*
dc.date.updated	2022-11-03T07:26:47Z
pubs.issue	11
pubs.publication-status	Published
pubs.volume	16
utslib.citation.issue	11

Abstract:

Heterotrophic bacterial diazotrophs (HBDs) are ubiquitous in the pelagic ocean, where they have been predicted to carry out the anaerobic process of nitrogen fixation within low-oxygen microenvironments associated with marine pelagic particles. However, the mechanisms enabling particle colonization by HBDs are unknown. We hypothesized that HBDs use chemotaxis to locate and colonize suitable microenvironments, and showed that a cultivated marine HBD is chemotactic toward amino acids and phytoplankton-derived DOM. Using an in situ chemotaxis assay, we also discovered that diverse HBDs at a coastal site are motile and chemotactic toward DOM from various phytoplankton taxa and, indeed, that the proportion of diazotrophs was up to seven times higher among the motile fraction of the bacterial community compared to the bulk seawater community. Finally, three of four HBD isolates and 16 of 17 HBD metagenome assembled genomes, recovered from major ocean basins and locations along the Australian coast, each encoded >85% of proteins affiliated with the bacterial chemotaxis pathway. These results document the widespread capacity for chemotaxis in diverse and globally relevant marine HBDs. We suggest that HBDs could use chemotaxis to seek out and colonize low-oxygen microenvironments suitable for nitrogen fixation, such as those formed on marine particles. Chemotaxis in HBDs could therefore affect marine nitrogen and carbon biogeochemistry by facilitating nitrogen fixation within otherwise oxic waters, while also altering particle degradation and the efficiency of the biological pump.

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http://hdl.handle.net/10453/163211