Micronutrient content drives elementome variability amongst the Symbiodiniaceae.

Camp, EF; Nitschke, MR; Clases, D; Gonzalez de Vega, R; Reich, HG; Goyen, S; Suggett, DJ

Micronutrient content drives elementome variability amongst the Symbiodiniaceae.

Camp, EF Nitschke, MR Clases, D Gonzalez de Vega, R

Reich, HG Goyen, S

Suggett, DJ

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Publisher:: BioMed Central
Publication Type:: Journal Article
Citation:: BMC Plant Biology, 2022, 22, (1), pp. 1-14
Issue Date:: 2022-04-09

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Field	Value	Language
dc.contributor.author	Camp, EF
dc.contributor.author	Nitschke, MR
dc.contributor.author	Clases, D
dc.contributor.author	Gonzalez de Vega, R https://orcid.org/0000-0002-9806-9633
dc.contributor.author	Reich, HG
dc.contributor.author	Goyen, S https://orcid.org/0000-0003-3691-4640
dc.contributor.author	Suggett, DJ
dc.date.accessioned	2022-11-07T03:29:32Z
dc.date.available	2022-03-06
dc.date.available	2022-11-07T03:29:32Z
dc.date.issued	2022-04-09
dc.identifier.citation	BMC Plant Biology, 2022, 22, (1), pp. 1-14
dc.identifier.issn	1471-2229
dc.identifier.issn	1471-2229
dc.identifier.uri	http://hdl.handle.net/10453/163276
dc.description.abstract	BACKGROUND: Elements are the basis of life on Earth, whereby organisms are essentially evolved chemical substances that dynamically interact with each other and their environment. Determining species elemental quotas (their elementome) is a key indicator for their success across environments with different resource availabilities. Elementomes remain undescribed for functionally diverse dinoflagellates within the family Symbiodiniaceae that includes coral endosymbionts. We used dry combustion and ICP-MS to assess whether Symbiodiniaceae (ten isolates spanning five genera Breviolum, Cladocopium, Durusdinium, Effrenium, Symbiodinium) maintained under long-term nutrient replete conditions have unique elementomes (six key macronutrients and nine micronutrients) that would reflect evolutionarily conserved preferential elemental acquisition. For three isolates we assessed how elevated temperature impacted their elementomes. Further, we tested whether Symbiodiniaceae conform to common stoichiometric hypotheses (e.g., the growth rate hypothesis) documented in other marine algae. This study considers whether Symbiodiniaceae isolates possess unique elementomes reflective of their natural ecologies, evolutionary histories, and resistance to environmental change. RESULTS: Symbiodiniaceae isolates maintained under long-term luxury uptake conditions, all exhibited highly divergent elementomes from one another, driven primarily by differential content of micronutrients. All N:P and C:P ratios were below the Redfield ratio values, whereas C:N was close to the Redfield value. Elevated temperature resulted in a more homogenised elementome across isolates. The Family-level elementome was (C19.8N2.6 P1.0S18.8K0.7Ca0.1) · 1000 (Fe55.7Mn5.6Sr2.3Zn0.8Ni0.5Se0.3Cu0.2Mo0.1V0.04) mmol Phosphorous-1 versus (C25.4N3.1P1.0S23.1K0.9Ca0.4) · 1000 (Fe66.7Mn6.3Sr7.2Zn0.8Ni0.4Se0.2Cu0.2Mo0.2V0.05) mmol Phosphorous -1 at 27.4 ± 0.4 °C and 30.7 ± 0.01 °C, respectively. Symbiodiniaceae isolates tested here conformed to some, but not all, stoichiometric principles. CONCLUSIONS: Elementomes for Symbiodiniaceae diverge from those reported for other marine algae, primarily via lower C:N:P and different micronutrient expressions. Long-term maintenance of Symbiodiniaceae isolates in culture under common nutrient replete conditions suggests isolates have evolutionary conserved preferential uptake for certain elements that allows these unique elementomes to be identified. Micronutrient content (normalised to phosphorous) commonly increased in the Symbiodiniaceae isolates in response to elevated temperature, potentially indicating a common elemental signature to warming.
dc.format	Electronic
dc.language	eng
dc.publisher	BioMed Central
dc.relation	http://purl.org/au-research/grants/arc/DE190100142
dc.relation.ispartof	BMC Plant Biology
dc.relation.isbasedon	10.1186/s12870-022-03512-0
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0605 Microbiology, 0607 Plant Biology, 0703 Crop and Pasture Production
dc.subject.classification	Plant Biology & Botany
dc.subject.mesh	Animals
dc.subject.mesh	Anthozoa
dc.subject.mesh	Dinoflagellida
dc.subject.mesh	Micronutrients
dc.subject.mesh	Symbiosis
dc.subject.mesh	Animals
dc.subject.mesh	Anthozoa
dc.subject.mesh	Dinoflagellida
dc.subject.mesh	Micronutrients
dc.subject.mesh	Symbiosis
dc.subject.mesh	Animals
dc.subject.mesh	Anthozoa
dc.subject.mesh	Dinoflagellida
dc.subject.mesh	Micronutrients
dc.subject.mesh	Symbiosis
dc.title	Micronutrient content drives elementome variability amongst the Symbiodiniaceae.
dc.type	Journal Article
utslib.citation.volume	22
utslib.location.activity	England
utslib.for	0605 Microbiology
utslib.for	0607 Plant Biology
utslib.for	0703 Crop and Pasture Production
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Chemistry and Forensic Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2022-11-07T03:29:28Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	22
utslib.citation.issue	1

Abstract:

BACKGROUND: Elements are the basis of life on Earth, whereby organisms are essentially evolved chemical substances that dynamically interact with each other and their environment. Determining species elemental quotas (their elementome) is a key indicator for their success across environments with different resource availabilities. Elementomes remain undescribed for functionally diverse dinoflagellates within the family Symbiodiniaceae that includes coral endosymbionts. We used dry combustion and ICP-MS to assess whether Symbiodiniaceae (ten isolates spanning five genera Breviolum, Cladocopium, Durusdinium, Effrenium, Symbiodinium) maintained under long-term nutrient replete conditions have unique elementomes (six key macronutrients and nine micronutrients) that would reflect evolutionarily conserved preferential elemental acquisition. For three isolates we assessed how elevated temperature impacted their elementomes. Further, we tested whether Symbiodiniaceae conform to common stoichiometric hypotheses (e.g., the growth rate hypothesis) documented in other marine algae. This study considers whether Symbiodiniaceae isolates possess unique elementomes reflective of their natural ecologies, evolutionary histories, and resistance to environmental change. RESULTS: Symbiodiniaceae isolates maintained under long-term luxury uptake conditions, all exhibited highly divergent elementomes from one another, driven primarily by differential content of micronutrients. All N:P and C:P ratios were below the Redfield ratio values, whereas C:N was close to the Redfield value. Elevated temperature resulted in a more homogenised elementome across isolates. The Family-level elementome was (C19.8N2.6 P1.0S18.8K0.7Ca0.1) · 1000 (Fe55.7Mn5.6Sr2.3Zn0.8Ni0.5Se0.3Cu0.2Mo0.1V0.04) mmol Phosphorous-1 versus (C25.4N3.1P1.0S23.1K0.9Ca0.4) · 1000 (Fe66.7Mn6.3Sr7.2Zn0.8Ni0.4Se0.2Cu0.2Mo0.2V0.05) mmol Phosphorous -1 at 27.4 ± 0.4 °C and 30.7 ± 0.01 °C, respectively. Symbiodiniaceae isolates tested here conformed to some, but not all, stoichiometric principles. CONCLUSIONS: Elementomes for Symbiodiniaceae diverge from those reported for other marine algae, primarily via lower C:N:P and different micronutrient expressions. Long-term maintenance of Symbiodiniaceae isolates in culture under common nutrient replete conditions suggests isolates have evolutionary conserved preferential uptake for certain elements that allows these unique elementomes to be identified. Micronutrient content (normalised to phosphorous) commonly increased in the Symbiodiniaceae isolates in response to elevated temperature, potentially indicating a common elemental signature to warming.

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http://hdl.handle.net/10453/163276