Generation of Synechocystis sp. PCC 6803 mutant with enhanced laccase-like activity

Hurtado-McCormick, V; Commault, A; Herdean, A; Price, S; Pernice, M; Ralph, P

Generation of Synechocystis sp. PCC 6803 mutant with enhanced laccase-like activity

Hurtado-McCormick, V Commault, A Herdean, A Price, S Pernice, M

Ralph, P

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Bioresource Technology Reports, 2022, 20, pp. 101266
Issue Date:: 2022-11-01

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Field	Value	Language
dc.contributor.author	Hurtado-McCormick, V
dc.contributor.author	Commault, A
dc.contributor.author	Herdean, A
dc.contributor.author	Price, S
dc.contributor.author	Pernice, M https://orcid.org/0000-0002-3431-2104
dc.contributor.author	Ralph, P
dc.date.accessioned	2022-11-11T03:52:33Z
dc.date.available	2022-11-11T03:52:33Z
dc.date.issued	2022-11-01
dc.identifier.citation	Bioresource Technology Reports, 2022, 20, pp. 101266
dc.identifier.issn	2589-014X
dc.identifier.uri	http://hdl.handle.net/10453/163392
dc.description.abstract	Cyanobacteria offer a good alternative to fungi for laccase production at industrial scales. Random mutagenesis approaches with ethyl methanesulfonate were used in combination with enzymatic assays screenings to select a mutant of Synechocystis sp., A2, with enhanced extracellular laccase-like activity. Anthraquinone dye decolourisation assay revealed 7 % enhanced decolourisation in A2 relative to wild type after 24 h. Comparison of the microbiome composition, structure and richness of the wild type and A2 strains confirmed that the improved traits were due to the mutation(s) and not the associated bacteria. The newly isolated Synechocystis mutant is the first example of successful random mutagenesis of cyanobacteria for laccase production. Attempts to characterise the biochemical reaction and putative laccase in A2 strain were unsuccessful but will be the subject of further research. This study suggests that random mutagenesis as a powerful approach for generating cyanobacterial strains with enhanced laccase-like activity for prospective commercial applications.
dc.language	en
dc.publisher	Elsevier BV
dc.relation.ispartof	Bioresource Technology Reports
dc.relation.isbasedon	10.1016/j.biteb.2022.101266
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.title	Generation of Synechocystis sp. PCC 6803 mutant with enhanced laccase-like activity
dc.type	Journal Article
utslib.citation.volume	20
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access	*
utslib.copyright.embargo	2024-12-01T00:00:00+1000Z
dc.date.updated	2022-11-11T03:52:27Z
pubs.publication-status	Published
pubs.volume	20

Abstract:

Cyanobacteria offer a good alternative to fungi for laccase production at industrial scales. Random mutagenesis approaches with ethyl methanesulfonate were used in combination with enzymatic assays screenings to select a mutant of Synechocystis sp., A2, with enhanced extracellular laccase-like activity. Anthraquinone dye decolourisation assay revealed 7 % enhanced decolourisation in A2 relative to wild type after 24 h. Comparison of the microbiome composition, structure and richness of the wild type and A2 strains confirmed that the improved traits were due to the mutation(s) and not the associated bacteria. The newly isolated Synechocystis mutant is the first example of successful random mutagenesis of cyanobacteria for laccase production. Attempts to characterise the biochemical reaction and putative laccase in A2 strain were unsuccessful but will be the subject of further research. This study suggests that random mutagenesis as a powerful approach for generating cyanobacterial strains with enhanced laccase-like activity for prospective commercial applications.

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http://hdl.handle.net/10453/163392