Consuming fresh macroalgae induces specific catabolic pathways, stress reactions and Type IX secretion in marine flavobacterial pioneer degraders.

Brunet, M; Le Duff, N; Barbeyron, T; Thomas, F

Consuming fresh macroalgae induces specific catabolic pathways, stress reactions and Type IX secretion in marine flavobacterial pioneer degraders.

Brunet, M

Le Duff, N Barbeyron, T Thomas, F

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Publication Type:: Journal Article
Citation:: ISME J, 2022, 16, (8), pp. 2027-2039
Issue Date:: 2022-08

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Field	Value	Language
dc.contributor.author	Brunet, M https://orcid.org/0000-0003-4245-2075
dc.contributor.author	Le Duff, N
dc.contributor.author	Barbeyron, T
dc.contributor.author	Thomas, F
dc.date.accessioned	2022-11-14T02:55:07Z
dc.date.available	2022-05-09
dc.date.available	2022-11-14T02:55:07Z
dc.date.issued	2022-08
dc.identifier.citation	ISME J, 2022, 16, (8), pp. 2027-2039
dc.identifier.issn	1751-7362
dc.identifier.issn	1751-7370
dc.identifier.uri	http://hdl.handle.net/10453/163433
dc.description.abstract	Macroalgae represent huge amounts of biomass worldwide, largely recycled by marine heterotrophic bacteria. We investigated the strategies of bacteria within the flavobacterial genus Zobellia to initiate the degradation of whole algal tissues, which has received little attention compared to the degradation of isolated polysaccharides. Zobellia galactanivorans DsijT has the capacity to use fresh brown macroalgae as a sole carbon source and extensively degrades algal tissues via the secretion of extracellular enzymes, even in the absence of physical contact with the algae. Co-cultures experiments with the non-degrading strain Tenacibaculum aestuarii SMK-4T showed that Z. galactanivorans can act as a pioneer that initiates algal breakdown and shares public goods with other bacteria. A comparison of eight Zobellia strains, and strong transcriptomic shifts in Z. galactanivorans cells using fresh macroalgae vs. isolated polysaccharides, revealed potential overlooked traits of pioneer bacteria. Besides brown algal polysaccharide degradation, they notably include oxidative stress resistance proteins, type IX secretion system proteins and novel uncharacterized polysaccharide utilization loci. Overall, this work highlights the relevance of studying fresh macroalga degradation to fully understand the metabolic and ecological strategies of pioneer microbial degraders, key players in macroalgal biomass remineralization.
dc.format	Print-Electronic
dc.language	eng
dc.relation.ispartof	ISME J
dc.relation.isbasedon	10.1038/s41396-022-01251-6
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	05 Environmental Sciences, 06 Biological Sciences, 10 Technology
dc.subject.classification	Microbiology
dc.subject.mesh	Carbohydrate Metabolism
dc.subject.mesh	Polysaccharides
dc.subject.mesh	Seaweed
dc.subject.mesh	Seaweed
dc.subject.mesh	Polysaccharides
dc.subject.mesh	Carbohydrate Metabolism
dc.title	Consuming fresh macroalgae induces specific catabolic pathways, stress reactions and Type IX secretion in marine flavobacterial pioneer degraders.
dc.type	Journal Article
utslib.citation.volume	16
utslib.location.activity	England
utslib.for	05 Environmental Sciences
utslib.for	06 Biological Sciences
utslib.for	10 Technology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.date.updated	2022-11-14T02:55:02Z
pubs.issue	8
pubs.publication-status	Published
pubs.volume	16
utslib.citation.issue	8

Abstract:

Macroalgae represent huge amounts of biomass worldwide, largely recycled by marine heterotrophic bacteria. We investigated the strategies of bacteria within the flavobacterial genus Zobellia to initiate the degradation of whole algal tissues, which has received little attention compared to the degradation of isolated polysaccharides. Zobellia galactanivorans DsijT has the capacity to use fresh brown macroalgae as a sole carbon source and extensively degrades algal tissues via the secretion of extracellular enzymes, even in the absence of physical contact with the algae. Co-cultures experiments with the non-degrading strain Tenacibaculum aestuarii SMK-4T showed that Z. galactanivorans can act as a pioneer that initiates algal breakdown and shares public goods with other bacteria. A comparison of eight Zobellia strains, and strong transcriptomic shifts in Z. galactanivorans cells using fresh macroalgae vs. isolated polysaccharides, revealed potential overlooked traits of pioneer bacteria. Besides brown algal polysaccharide degradation, they notably include oxidative stress resistance proteins, type IX secretion system proteins and novel uncharacterized polysaccharide utilization loci. Overall, this work highlights the relevance of studying fresh macroalga degradation to fully understand the metabolic and ecological strategies of pioneer microbial degraders, key players in macroalgal biomass remineralization.

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http://hdl.handle.net/10453/163433