Comparison of operational DNA recovery methods: Swabs versus tapelifts

Burmuzoska, I; Hogg, K; Raymond, J; Hitchcock, C; Meakin, GE

Comparison of operational DNA recovery methods: Swabs versus tapelifts

Burmuzoska, I Hogg, K Raymond, J Hitchcock, C Meakin, GE

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Forensic Science International: Genetics Supplement Series, 2022
Issue Date:: 2022-01-01

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Field	Value	Language
dc.contributor.author	Burmuzoska, I
dc.contributor.author	Hogg, K
dc.contributor.author	Raymond, J
dc.contributor.author	Hitchcock, C
dc.contributor.author	Meakin, GE
dc.date.accessioned	2022-11-24T00:54:37Z
dc.date.available	2022-11-24T00:54:37Z
dc.date.issued	2022-01-01
dc.identifier.citation	Forensic Science International: Genetics Supplement Series, 2022
dc.identifier.issn	1875-1768
dc.identifier.issn	1875-175X
dc.identifier.uri	http://hdl.handle.net/10453/163704
dc.description.abstract	It is routine among many jurisdictions to recover DNA using tapelifts on porous substrates (e.g. clothing) and swabs on non-porous substrates (e.g. tool handles). Here, we examine this by comparing the efficiency of the NSW jurisdiction's specific swabbing and tapelift techniques on a range of porous and non-porous substrates. To test DNA recovery efficiency, 30 μl aliquots of 1:50 and 1:100 saliva dilutions were deposited onto the substrates, left to dry overnight, recovered, extracted, quantified and a subset profiled. Tapelifts recovered more DNA and DNA profiles with more detectable alleles than swabs for both saliva dilutions on porous substrates. For non-porous substrates, similar DNA quantities and profiles were generally recovered with both methods for both saliva dilutions. These data underpin current practices to recover DNA using tapelifts for porous substrates and swabs for non-porous substrates. These data also revealed severe degradation of DNA recovered from brass, supporting the on-going need to improve DNA recovery and analysis methods for brass substrates.
dc.language	en
dc.publisher	Elsevier BV
dc.relation.ispartof	Forensic Science International: Genetics Supplement Series
dc.relation.isbasedon	10.1016/j.fsigss.2022.09.019
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.subject.classification	Legal & Forensic Medicine
dc.title	Comparison of operational DNA recovery methods: Swabs versus tapelifts
dc.type	Journal Article
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access	*
utslib.copyright.embargo	2023-09-01T00:00:00+1000Z
dc.date.updated	2022-11-24T00:54:36Z
pubs.publication-status	Published

Abstract:

It is routine among many jurisdictions to recover DNA using tapelifts on porous substrates (e.g. clothing) and swabs on non-porous substrates (e.g. tool handles). Here, we examine this by comparing the efficiency of the NSW jurisdiction's specific swabbing and tapelift techniques on a range of porous and non-porous substrates. To test DNA recovery efficiency, 30 μl aliquots of 1:50 and 1:100 saliva dilutions were deposited onto the substrates, left to dry overnight, recovered, extracted, quantified and a subset profiled. Tapelifts recovered more DNA and DNA profiles with more detectable alleles than swabs for both saliva dilutions on porous substrates. For non-porous substrates, similar DNA quantities and profiles were generally recovered with both methods for both saliva dilutions. These data underpin current practices to recover DNA using tapelifts for porous substrates and swabs for non-porous substrates. These data also revealed severe degradation of DNA recovered from brass, supporting the on-going need to improve DNA recovery and analysis methods for brass substrates.

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http://hdl.handle.net/10453/163704