Assessing an Adaptation of the Universal Parasite Diagnostic Assay for Bloodborne Parasites in a US State Public Health Laboratory.

Clemons, B; Barratt, J; Lane, M; Qvarnstrom, Y; Teal, AE; Zayas, G; Madison-Antenucci, S

Assessing an Adaptation of the Universal Parasite Diagnostic Assay for Bloodborne Parasites in a US State Public Health Laboratory.

Clemons, B Barratt, J

Lane, M Qvarnstrom, Y Teal, AE Zayas, G Madison-Antenucci, S

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Publisher:: American Society of Tropical Medicine and Hygiene
Publication Type:: Journal Article
Citation:: American Journal of Tropical Medicine and Hygiene, 2022, 106, (2), pp. 671-677
Issue Date:: 2022-01-01

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Field	Value	Language
dc.contributor.author	Clemons, B
dc.contributor.author	Barratt, J https://orcid.org/0000-0001-8711-2408
dc.contributor.author	Lane, M
dc.contributor.author	Qvarnstrom, Y
dc.contributor.author	Teal, AE
dc.contributor.author	Zayas, G
dc.contributor.author	Madison-Antenucci, S
dc.date.accessioned	2022-12-06T02:27:50Z
dc.date.available	2021-09-15
dc.date.available	2022-12-06T02:27:50Z
dc.date.issued	2022-01-01
dc.identifier.citation	American Journal of Tropical Medicine and Hygiene, 2022, 106, (2), pp. 671-677
dc.identifier.issn	0002-9637
dc.identifier.issn	1476-1645
dc.identifier.uri	http://hdl.handle.net/10453/164145
dc.description.abstract	For complex clinical cases where a parasitic infection is suspected, it can be difficult for clinicians to recommend an appropriate laboratory test. These tests are usually pathogen-specific and require a certain degree of suspicion for the precise etiology. Recently, Flaherty et al. (2021) described an assay, the universal parasite diagnostic (UPDx) that can potentially provide a diagnosis of any parasite present in a specimen. Using primers that amplify DNA from all eukaryotes, UPDx differentiates several parasitic infections in blood by amplicon-based next-generation sequencing (NGS) of the 18S rDNA locus. As the state's public health reference laboratory, the Parasitology Laboratory at the Wadsworth Center (New York, NY) receives specimens from patients who have potentially encountered a wide variety of parasites. As such, the ability to differentiate several blood parasites using a single assay is of interest. We assessed UPDx for its ability to confirm parasitic infections for 20 specimens that were previously identified by real-time PCR (RT-PCR). This included specimens positive for Babesia microti, Trypanosoma cruzi, Leishmania tropica, various Plasmodium species, and specimens comprising mixed Plasmodium sp. infections. Results obtained using UPDx were largely concordant with the RT-PCR assays. A T. cruzi positive specimen was negative by UPDx and for two mixed Plasmodium sp. infections only one species was detected. The results obtained for other specimens were concordant. We conclude that UPDx shows promise for the detection of blood parasites in diagnostic laboratories. As NGS becomes cheaper, assays like UPDx will become increasingly amenable to use in clinical settings.
dc.format	Electronic
dc.language	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartof	American Journal of Tropical Medicine and Hygiene
dc.relation.isbasedon	10.4269/ajtmh.21-0707
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	11 Medical and Health Sciences
dc.subject.classification	Tropical Medicine
dc.subject.mesh	Blood-Borne Infections
dc.subject.mesh	High-Throughput Nucleotide Sequencing
dc.subject.mesh	Humans
dc.subject.mesh	Laboratories
dc.subject.mesh	Molecular Diagnostic Techniques
dc.subject.mesh	Parasitic Diseases
dc.subject.mesh	Public Health
dc.subject.mesh	RNA, Ribosomal, 18S
dc.subject.mesh	United States
dc.subject.mesh	Humans
dc.subject.mesh	Parasitic Diseases
dc.subject.mesh	RNA, Ribosomal, 18S
dc.subject.mesh	Molecular Diagnostic Techniques
dc.subject.mesh	Public Health
dc.subject.mesh	Laboratories
dc.subject.mesh	United States
dc.subject.mesh	High-Throughput Nucleotide Sequencing
dc.subject.mesh	Blood-Borne Infections
dc.title	Assessing an Adaptation of the Universal Parasite Diagnostic Assay for Bloodborne Parasites in a US State Public Health Laboratory.
dc.type	Journal Article
utslib.citation.volume	106
utslib.location.activity	United States
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-12-06T02:27:48Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	106
utslib.citation.issue	2

Abstract:

For complex clinical cases where a parasitic infection is suspected, it can be difficult for clinicians to recommend an appropriate laboratory test. These tests are usually pathogen-specific and require a certain degree of suspicion for the precise etiology. Recently, Flaherty et al. (2021) described an assay, the universal parasite diagnostic (UPDx) that can potentially provide a diagnosis of any parasite present in a specimen. Using primers that amplify DNA from all eukaryotes, UPDx differentiates several parasitic infections in blood by amplicon-based next-generation sequencing (NGS) of the 18S rDNA locus. As the state's public health reference laboratory, the Parasitology Laboratory at the Wadsworth Center (New York, NY) receives specimens from patients who have potentially encountered a wide variety of parasites. As such, the ability to differentiate several blood parasites using a single assay is of interest. We assessed UPDx for its ability to confirm parasitic infections for 20 specimens that were previously identified by real-time PCR (RT-PCR). This included specimens positive for Babesia microti, Trypanosoma cruzi, Leishmania tropica, various Plasmodium species, and specimens comprising mixed Plasmodium sp. infections. Results obtained using UPDx were largely concordant with the RT-PCR assays. A T. cruzi positive specimen was negative by UPDx and for two mixed Plasmodium sp. infections only one species was detected. The results obtained for other specimens were concordant. We conclude that UPDx shows promise for the detection of blood parasites in diagnostic laboratories. As NGS becomes cheaper, assays like UPDx will become increasingly amenable to use in clinical settings.

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http://hdl.handle.net/10453/164145