Proteomic and Metabolomic Signatures Associated With the Immune Response in Healthy Individuals Immunized With an Inactivated SARS-CoV-2 Vaccine.

Wang, Y; Wang, X; Luu, LDW; Chen, S; Jin, F; Wang, S; Huang, X; Wang, L; Zhou, X; Chen, X; Cui, X; Li, J; Tai, J; Zhu, X

Proteomic and Metabolomic Signatures Associated With the Immune Response in Healthy Individuals Immunized With an Inactivated SARS-CoV-2 Vaccine.

Wang, Y Wang, X Luu, LDW Chen, S Jin, F Wang, S Huang, X Wang, L Zhou, X Chen, X Cui, X Li, J Tai, J Zhu, X

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Publisher:: Frontiers Media
Publication Type:: Journal Article
Citation:: Frontiers in Immunology, 2022, 13, pp. 1-15
Issue Date:: 2022

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Field	Value	Language
dc.contributor.author	Wang, Y
dc.contributor.author	Wang, X
dc.contributor.author	Luu, LDW
dc.contributor.author	Chen, S
dc.contributor.author	Jin, F
dc.contributor.author	Wang, S
dc.contributor.author	Huang, X
dc.contributor.author	Wang, L
dc.contributor.author	Zhou, X
dc.contributor.author	Chen, X
dc.contributor.author	Cui, X
dc.contributor.author	Li, J
dc.contributor.author	Tai, J
dc.contributor.author	Zhu, X
dc.date.accessioned	2022-12-07T04:36:25Z
dc.date.available	2022-04-20
dc.date.available	2022-12-07T04:36:25Z
dc.date.issued	2022
dc.identifier.citation	Frontiers in Immunology, 2022, 13, pp. 1-15
dc.identifier.issn	1664-3224
dc.identifier.issn	1664-3224
dc.identifier.uri	http://hdl.handle.net/10453/164190
dc.description.abstract	CoronaVac (Sinovac), an inactivated vaccine for SARS-CoV-2, has been widely used for immunization. However, analysis of the underlying molecular mechanisms driving CoronaVac-induced immunity is still limited. Here, we applied a systems biology approach to understand the mechanisms behind the adaptive immune response to CoronaVac in a cohort of 50 volunteers immunized with 2 doses of CoronaVac. Vaccination with CoronaVac led to an integrated immune response that included several effector arms of the adaptive immune system including specific IgM/IgG, humoral response and other immune response, as well as the innate immune system as shown by complement activation. Metabolites associated with immunity were also identified implicating the role of metabolites in the humoral response, complement activation and other immune response. Networks associated with the TCA cycle and amino acids metabolic pathways, such as phenylalanine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and glycine, serine and threonine metabolism were tightly coupled with immunity. Critically, we constructed a multifactorial response network (MRN) to analyze the underlying interactions and compared the signatures affected by CoronaVac immunization and SARS-CoV-2 infection to further identify immune signatures and related metabolic pathways altered by CoronaVac immunization. These results help us to understand the host response to vaccination of CoronaVac and highlight the utility of a systems biology approach in defining molecular correlates of protection to vaccination.
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	Frontiers Media
dc.relation.ispartof	Frontiers in Immunology
dc.relation.isbasedon	10.3389/fimmu.2022.848961
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1107 Immunology, 1108 Medical Microbiology
dc.subject.mesh	Adaptive Immunity
dc.subject.mesh	COVID-19
dc.subject.mesh	COVID-19 Vaccines
dc.subject.mesh	Humans
dc.subject.mesh	Phenylalanine
dc.subject.mesh	Proteomics
dc.subject.mesh	SARS-CoV-2
dc.subject.mesh	Vaccines, Inactivated
dc.subject.mesh	Viral Vaccines
dc.subject.mesh	Adaptive Immunity
dc.subject.mesh	COVID-19
dc.subject.mesh	COVID-19 Vaccines
dc.subject.mesh	Humans
dc.subject.mesh	Phenylalanine
dc.subject.mesh	Proteomics
dc.subject.mesh	SARS-CoV-2
dc.subject.mesh	Vaccines, Inactivated
dc.subject.mesh	Viral Vaccines
dc.subject.mesh	Humans
dc.subject.mesh	Phenylalanine
dc.subject.mesh	Vaccines, Inactivated
dc.subject.mesh	Viral Vaccines
dc.subject.mesh	Proteomics
dc.subject.mesh	Adaptive Immunity
dc.subject.mesh	COVID-19
dc.subject.mesh	SARS-CoV-2
dc.subject.mesh	COVID-19 Vaccines
dc.title	Proteomic and Metabolomic Signatures Associated With the Immune Response in Healthy Individuals Immunized With an Inactivated SARS-CoV-2 Vaccine.
dc.type	Journal Article
utslib.citation.volume	13
utslib.location.activity	Switzerland
utslib.for	1107 Immunology
utslib.for	1108 Medical Microbiology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2022-12-07T04:34:35Z
pubs.publication-status	Published
pubs.volume	13

Abstract:

CoronaVac (Sinovac), an inactivated vaccine for SARS-CoV-2, has been widely used for immunization. However, analysis of the underlying molecular mechanisms driving CoronaVac-induced immunity is still limited. Here, we applied a systems biology approach to understand the mechanisms behind the adaptive immune response to CoronaVac in a cohort of 50 volunteers immunized with 2 doses of CoronaVac. Vaccination with CoronaVac led to an integrated immune response that included several effector arms of the adaptive immune system including specific IgM/IgG, humoral response and other immune response, as well as the innate immune system as shown by complement activation. Metabolites associated with immunity were also identified implicating the role of metabolites in the humoral response, complement activation and other immune response. Networks associated with the TCA cycle and amino acids metabolic pathways, such as phenylalanine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and glycine, serine and threonine metabolism were tightly coupled with immunity. Critically, we constructed a multifactorial response network (MRN) to analyze the underlying interactions and compared the signatures affected by CoronaVac immunization and SARS-CoV-2 infection to further identify immune signatures and related metabolic pathways altered by CoronaVac immunization. These results help us to understand the host response to vaccination of CoronaVac and highlight the utility of a systems biology approach in defining molecular correlates of protection to vaccination.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/164190