Antioxidant and antibacterial activities of different solvent extracts of citrus acida leaf and correlation with phenolic content

Jain, P; Hossain, KR; Islam, T; Gias, ZT; Hossain, M; Reza, HM

Antioxidant and antibacterial activities of different solvent extracts of citrus acida leaf and correlation with phenolic content

Jain, P Hossain, KR Islam, T Gias, ZT Hossain, M Reza, HM

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Publisher:: Diva Enterprises Private Limited
Publication Type:: Journal Article
Citation:: Medicinal Plants, 2021, 13, (2), pp. 302-312
Issue Date:: 2021-06-01

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Field	Value	Language
dc.contributor.author	Jain, P
dc.contributor.author	Hossain, KR
dc.contributor.author	Islam, T
dc.contributor.author	Gias, ZT
dc.contributor.author	Hossain, M
dc.contributor.author	Reza, HM
dc.date.accessioned	2023-01-09T05:15:43Z
dc.date.available	2023-01-09T05:15:43Z
dc.date.issued	2021-06-01
dc.identifier.citation	Medicinal Plants, 2021, 13, (2), pp. 302-312
dc.identifier.issn	0975-4261
dc.identifier.issn	0975-6892
dc.identifier.uri	http://hdl.handle.net/10453/164788
dc.description.abstract	Plants belonging to Citrus sp. are rich in phenolics which are of great medicinal value. The present study focuses on antioxidant and antibacterial potential of various extracts of a locally grown variety of Citrus acida leaves and their correlation with phenolic content. Multiple in-vitro models have been used to evaluate the free radical scavenging potential. Ethanol extract showed the highest DPPH (1,1-diphenyl-2-picrylhydrazyl), nitric oxide, superoxide scavenging and iron chelation activity with IC50 values 40.44±1.41, 132.89±4.69, 94.64±4.44 and 281.88±6.49 µg/ml, respectively. The highest ABTS (2, 2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) and hydrogen peroxide scavenging activities with IC50 value 34.95±1.23 and 117.50±2.70 µg/ml, respectively were exhibited by hexane extract. Dose dependent reducing power was observed with all the extracts. The antioxidant potential of the extracts showed significant correlation with the total phenolic content in four assay models with Pearson’s correlation coefficient (r) values ranging from 0.846-0.989. The extracts showed antibacterial activity against all the test isolates with zone of inhibition ranging from 7.33±1.53 to 28.00±1.00 mm. Maximum antibacterial activity was recorded by ethyl acetate extract against Pseudomonas aeruginosa. Ethyl acetate and ethanol extract exhibited the lowest MIC values of 2.5 mg/ml against Shigella dysenteriae and Vibrio mimicus, respectively. The results suggest that citrus leaves can play a promising role in the development of herbal drugs with antioxidant and antibacterial activities.
dc.language	en
dc.publisher	Diva Enterprises Private Limited
dc.relation.ispartof	Medicinal Plants
dc.relation.isbasedon	10.5958/0975-6892.2021.00034.4
dc.rights	info:eu-repo/semantics/closedAccess
dc.title	Antioxidant and antibacterial activities of different solvent extracts of citrus acida leaf and correlation with phenolic content
dc.type	Journal Article
utslib.citation.volume	13
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2023-01-09T05:15:41Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	13
utslib.citation.issue	2

Abstract:

Plants belonging to Citrus sp. are rich in phenolics which are of great medicinal value. The present study focuses on antioxidant and antibacterial potential of various extracts of a locally grown variety of Citrus acida leaves and their correlation with phenolic content. Multiple in-vitro models have been used to evaluate the free radical scavenging potential. Ethanol extract showed the highest DPPH (1,1-diphenyl-2-picrylhydrazyl), nitric oxide, superoxide scavenging and iron chelation activity with IC50 values 40.44±1.41, 132.89±4.69, 94.64±4.44 and 281.88±6.49 µg/ml, respectively. The highest ABTS (2, 2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) and hydrogen peroxide scavenging activities with IC50 value 34.95±1.23 and 117.50±2.70 µg/ml, respectively were exhibited by hexane extract. Dose dependent reducing power was observed with all the extracts. The antioxidant potential of the extracts showed significant correlation with the total phenolic content in four assay models with Pearson’s correlation coefficient (r) values ranging from 0.846-0.989. The extracts showed antibacterial activity against all the test isolates with zone of inhibition ranging from 7.33±1.53 to 28.00±1.00 mm. Maximum antibacterial activity was recorded by ethyl acetate extract against Pseudomonas aeruginosa. Ethyl acetate and ethanol extract exhibited the lowest MIC values of 2.5 mg/ml against Shigella dysenteriae and Vibrio mimicus, respectively. The results suggest that citrus leaves can play a promising role in the development of herbal drugs with antioxidant and antibacterial activities.

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http://hdl.handle.net/10453/164788