Timothy Grass Pollen Induces Spatial Reorganisation of F-Actin and Loss of Junctional Integrity in Respiratory Cells.

Bradbury, P; Cidem, A; Mahmodi, H; Davies, JM; Spicer, PT; Prescott, SW; Kabakova, I; Ong, HX; Traini, D

Timothy Grass Pollen Induces Spatial Reorganisation of F-Actin and Loss of Junctional Integrity in Respiratory Cells.

Bradbury, P Cidem, A Mahmodi, H Davies, JM Spicer, PT Prescott, SW Kabakova, I

Ong, HX Traini, D

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Publisher:: SPRINGER/PLENUM PUBLISHERS
Publication Type:: Journal Article
Citation:: Inflammation, 2022, 45, (3), pp. 1209-1223
Issue Date:: 2022-06

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Field	Value	Language
dc.contributor.author	Bradbury, P
dc.contributor.author	Cidem, A
dc.contributor.author	Mahmodi, H
dc.contributor.author	Davies, JM
dc.contributor.author	Spicer, PT
dc.contributor.author	Prescott, SW
dc.contributor.author	Kabakova, I https://orcid.org/0000-0002-6831-9478
dc.contributor.author	Ong, HX
dc.contributor.author	Traini, D
dc.date.accessioned	2023-01-13T02:54:14Z
dc.date.available	2021-12-13
dc.date.available	2023-01-13T02:54:14Z
dc.date.issued	2022-06
dc.identifier.citation	Inflammation, 2022, 45, (3), pp. 1209-1223
dc.identifier.issn	0360-3997
dc.identifier.issn	1573-2576
dc.identifier.uri	http://hdl.handle.net/10453/164952
dc.description.abstract	Grass pollens have been identified as mediators of respiratory distress, capable of exacerbating respiratory diseases including epidemic thunderstorm asthma (ETSA). It is hypothesised that during thunderstorms, grass pollen grains swell to absorb atmospheric water, rupture, and release internal protein content to the atmosphere. The inhalation of atmospheric grass pollen proteins results in deadly ETSA events. We sought to identify the underlying cellular mechanisms that may contribute towards the severity of ETSA in temperate climates using Timothy grass (Phleum pratense). Respiratory cells exposed to Timothy grass pollen protein extract (PPE) caused cells to undergo hypoxia ultimately triggering the subcellular re-organisation of F-actin from the peri junctional belt to cytoplasmic fibre assembly traversing the cell body. This change in actin configuration coincided with the spatial reorganisation of microtubules and importantly, decreased cell compressibility specifically at the cell centre. Further to this, we find that the pollen-induced reorganisation of the actin cytoskeleton prompting secretion of the pro-inflammatory cytokine, interleukin-8. In addition, the loss of peri-junctional actin following exposure to pollen proteins was accompanied by the release of epithelial transmembrane protein, E-cadherin from cell-cell junctions resulting in a decrease in epithelial barrier integrity. We demonstrate that Timothy grass pollen regulates F-actin dynamics and E-cadherin localisation in respiratory cells to mediate cell-cell junctional integrity highlighting a possible molecular pathway underpinning ETSA events.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	SPRINGER/PLENUM PUBLISHERS
dc.relation	http://purl.org/au-research/grants/arc/DP190101973
dc.relation.ispartof	Inflammation
dc.relation.isbasedon	10.1007/s10753-021-01614-9
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	1107 Immunology
dc.subject.classification	Immunology
dc.subject.mesh	Actin Cytoskeleton
dc.subject.mesh	Actins
dc.subject.mesh	Allergens
dc.subject.mesh	Asthma
dc.subject.mesh	Cadherins
dc.subject.mesh	Humans
dc.subject.mesh	Phleum
dc.subject.mesh	Poaceae
dc.subject.mesh	Pollen
dc.subject.mesh	Humans
dc.subject.mesh	Poaceae
dc.subject.mesh	Phleum
dc.subject.mesh	Pollen
dc.subject.mesh	Asthma
dc.subject.mesh	Actins
dc.subject.mesh	Cadherins
dc.subject.mesh	Allergens
dc.subject.mesh	Actin Cytoskeleton
dc.title	Timothy Grass Pollen Induces Spatial Reorganisation of F-Actin and Loss of Junctional Integrity in Respiratory Cells.
dc.type	Journal Article
utslib.citation.volume	45
utslib.location.activity	United States
utslib.for	1107 Immunology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2023-01-13T02:54:08Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	45
utslib.citation.issue	3

Abstract:

Grass pollens have been identified as mediators of respiratory distress, capable of exacerbating respiratory diseases including epidemic thunderstorm asthma (ETSA). It is hypothesised that during thunderstorms, grass pollen grains swell to absorb atmospheric water, rupture, and release internal protein content to the atmosphere. The inhalation of atmospheric grass pollen proteins results in deadly ETSA events. We sought to identify the underlying cellular mechanisms that may contribute towards the severity of ETSA in temperate climates using Timothy grass (Phleum pratense). Respiratory cells exposed to Timothy grass pollen protein extract (PPE) caused cells to undergo hypoxia ultimately triggering the subcellular re-organisation of F-actin from the peri junctional belt to cytoplasmic fibre assembly traversing the cell body. This change in actin configuration coincided with the spatial reorganisation of microtubules and importantly, decreased cell compressibility specifically at the cell centre. Further to this, we find that the pollen-induced reorganisation of the actin cytoskeleton prompting secretion of the pro-inflammatory cytokine, interleukin-8. In addition, the loss of peri-junctional actin following exposure to pollen proteins was accompanied by the release of epithelial transmembrane protein, E-cadherin from cell-cell junctions resulting in a decrease in epithelial barrier integrity. We demonstrate that Timothy grass pollen regulates F-actin dynamics and E-cadherin localisation in respiratory cells to mediate cell-cell junctional integrity highlighting a possible molecular pathway underpinning ETSA events.

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http://hdl.handle.net/10453/164952