Genome-resolved metagenomics provides insights into the functional complexity of microbial mats in Blue Holes, Shark Bay.

Kindler, GS; Wong, HL; Larkum, AWD; Johnson, M; MacLeod, FI; Burns, BP

Genome-resolved metagenomics provides insights into the functional complexity of microbial mats in Blue Holes, Shark Bay.

Kindler, GS Wong, HL Larkum, AWD Johnson, M MacLeod, FI Burns, BP

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Publisher:: Federation of European Microbiological Societies
Publication Type:: Journal Article
Citation:: FEMS Microbiology Ecology, 2022, 98, (1), pp. fiab158
Issue Date:: 2022-01-19

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Field	Value	Language
dc.contributor.author	Kindler, GS
dc.contributor.author	Wong, HL
dc.contributor.author	Larkum, AWD
dc.contributor.author	Johnson, M
dc.contributor.author	MacLeod, FI
dc.contributor.author	Burns, BP
dc.date.accessioned	2023-01-17T04:47:14Z
dc.date.available	2021-11-30
dc.date.available	2023-01-17T04:47:14Z
dc.date.issued	2022-01-19
dc.identifier.citation	FEMS Microbiology Ecology, 2022, 98, (1), pp. fiab158
dc.identifier.issn	0168-6496
dc.identifier.issn	1574-6941
dc.identifier.uri	http://hdl.handle.net/10453/165063
dc.description.abstract	The present study describes for the first time the community composition and functional potential of the microbial mats found in the supratidal, gypsum-rich and hypersaline region of Blue Holes, Shark Bay. This was achieved via high-throughput metagenomic sequencing of total mat community DNA and complementary analyses using hyperspectral confocal microscopy. Mat communities were dominated by Proteobacteria (29%), followed by Bacteroidetes/Chlorobi group (11%) and Planctomycetes (10%). These mats were found to also harbour a diverse community of potentially novel microorganisms, including members from the DPANN, Asgard archaea and candidate phyla radiation, with highest diversity found in the lower regions (∼14-20 mm depth) of the mat. In addition to pathways for major metabolic cycles, a range of putative rhodopsins with previously uncharacterized motifs and functions were identified along with heliorhodopsins and putative schizorhodopsins. Critical microbial interactions were also inferred, and from 117 medium- to high-quality metagenome-assembled genomes, viral defence mechanisms (CRISPR, BREX and DISARM), elemental transport, osmoprotection, heavy metal resistance and UV resistance were also detected. These analyses have provided a greater understanding of these distinct mat systems in Shark Bay, including key insights into adaptive responses and proposing that photoheterotrophy may be an important lifestyle in Blue Holes.
dc.format	Print
dc.language	eng
dc.publisher	Federation of European Microbiological Societies
dc.relation.ispartof	FEMS Microbiology Ecology
dc.relation.isbasedon	10.1093/femsec/fiab158
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	05 Environmental Sciences, 06 Biological Sciences, 11 Medical and Health Sciences
dc.subject.classification	Microbiology
dc.subject.mesh	Animals
dc.subject.mesh	Archaea
dc.subject.mesh	Bays
dc.subject.mesh	Metagenomics
dc.subject.mesh	Phylogeny
dc.subject.mesh	Planctomycetes
dc.subject.mesh	Sharks
dc.subject.mesh	Animals
dc.subject.mesh	Sharks
dc.subject.mesh	Archaea
dc.subject.mesh	Phylogeny
dc.subject.mesh	Metagenomics
dc.subject.mesh	Bays
dc.subject.mesh	Planctomycetes
dc.title	Genome-resolved metagenomics provides insights into the functional complexity of microbial mats in Blue Holes, Shark Bay.
dc.type	Journal Article
utslib.citation.volume	98
utslib.location.activity	England
utslib.for	05 Environmental Sciences
utslib.for	06 Biological Sciences
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2023-01-17T04:44:46Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	98
utslib.citation.issue	1

Abstract:

The present study describes for the first time the community composition and functional potential of the microbial mats found in the supratidal, gypsum-rich and hypersaline region of Blue Holes, Shark Bay. This was achieved via high-throughput metagenomic sequencing of total mat community DNA and complementary analyses using hyperspectral confocal microscopy. Mat communities were dominated by Proteobacteria (29%), followed by Bacteroidetes/Chlorobi group (11%) and Planctomycetes (10%). These mats were found to also harbour a diverse community of potentially novel microorganisms, including members from the DPANN, Asgard archaea and candidate phyla radiation, with highest diversity found in the lower regions (∼14-20 mm depth) of the mat. In addition to pathways for major metabolic cycles, a range of putative rhodopsins with previously uncharacterized motifs and functions were identified along with heliorhodopsins and putative schizorhodopsins. Critical microbial interactions were also inferred, and from 117 medium- to high-quality metagenome-assembled genomes, viral defence mechanisms (CRISPR, BREX and DISARM), elemental transport, osmoprotection, heavy metal resistance and UV resistance were also detected. These analyses have provided a greater understanding of these distinct mat systems in Shark Bay, including key insights into adaptive responses and proposing that photoheterotrophy may be an important lifestyle in Blue Holes.

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http://hdl.handle.net/10453/165063