Characterization of the wave phenomenon of flash-induced chlorophyll fluorescence in Chlamydomonas reinhardtii.

Patil, PP; Mohammad Aslam, S; Vass, I; Szabó, M

Characterization of the wave phenomenon of flash-induced chlorophyll fluorescence in Chlamydomonas reinhardtii.

Patil, PP Mohammad Aslam, S Vass, I Szabó, M

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Publisher:: SPRINGER
Publication Type:: Journal Article
Citation:: Photosynth Res, 2022, 152, (2), pp. 235-244
Issue Date:: 2022-05

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Field	Value	Language
dc.contributor.author	Patil, PP
dc.contributor.author	Mohammad Aslam, S
dc.contributor.author	Vass, I
dc.contributor.author	Szabó, M
dc.date.accessioned	2023-02-01T00:12:35Z
dc.date.available	2022-01-25
dc.date.available	2023-02-01T00:12:35Z
dc.date.issued	2022-05
dc.identifier.citation	Photosynth Res, 2022, 152, (2), pp. 235-244
dc.identifier.issn	0166-8595
dc.identifier.issn	1573-5079
dc.identifier.uri	http://hdl.handle.net/10453/165715
dc.description.abstract	Flash-induced chlorophyll fluorescence relaxation is a powerful tool to monitor the reoxidation reactions of the reduced primary quinone acceptor, QA- by QB and the plastoquinone (PQ) pool, as well as the charge recombination reactions between the donor and acceptor side components of Photosystem II (PSII). Under certain conditions, when the PQ pool is highly reduced (e.g. in microaerobic conditions), a wave phenomenon appears in the fluorescence relaxation kinetics, which reflects the transient reoxidation and re-reduction of QA- by various electron transfer processes, which in cyanobacteria is mediated by NAD(P)H dehydrogenase (NDH-1). The wave phenomenon was also observed and assigned to the operation of type 2 NAD(P)H dehydrogenase (NDH-2) in the green alga Chlamydomonas reinhardtii under hydrogen-producing conditions, which required a long incubation of algae under sulphur deprivation (Krishna et al. J Exp Bot 70 (21):6321-6336, 2019). However, the conditions that induce the wave remained largely uncharacterized so far in microalgae. In this work, we investigated the wave phenomenon in Chlamydomonas reinhardtii under conditions that lead to a decrease of PSII activity by applying hydroxylamine treatment, which impacts the donor side of PSII in combination with a strongly reducing environment of the PQ pool (microaerobic conditions). A similar wave phenomenon could be induced by photoinhibitory conditions (illumination with strong light in the presence of the protein synthesis inhibitor lincomycin). These results indicate that the fluorescence wave phenomenon is activated in green algae when the PSII activity decreases relative to Photosystem I (PS I) activity and the PQ pool is strongly reduced. Therefore, the fluorescence wave could be used as a sensitive indicator of altered intersystem electron transfer processes, e.g. under stress conditions.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	SPRINGER
dc.relation.ispartof	Photosynth Res
dc.relation.isbasedon	10.1007/s11120-022-00900-3
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0601 Biochemistry and Cell Biology, 0604 Genetics, 0607 Plant Biology
dc.subject.classification	Plant Biology & Botany
dc.subject.mesh	Chlamydomonas reinhardtii
dc.subject.mesh	Chlorophyll
dc.subject.mesh	Electron Transport
dc.subject.mesh	Fluorescence
dc.subject.mesh	NAD
dc.subject.mesh	Oxidation-Reduction
dc.subject.mesh	Oxidoreductases
dc.subject.mesh	Photosystem II Protein Complex
dc.subject.mesh	Plastoquinone
dc.subject.mesh	Chlamydomonas reinhardtii
dc.subject.mesh	Plastoquinone
dc.subject.mesh	Chlorophyll
dc.subject.mesh	Photosystem II Protein Complex
dc.subject.mesh	NAD
dc.subject.mesh	Oxidoreductases
dc.subject.mesh	Electron Transport
dc.subject.mesh	Oxidation-Reduction
dc.subject.mesh	Fluorescence
dc.title	Characterization of the wave phenomenon of flash-induced chlorophyll fluorescence in Chlamydomonas reinhardtii.
dc.type	Journal Article
utslib.citation.volume	152
utslib.location.activity	Netherlands
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	0604 Genetics
utslib.for	0607 Plant Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.date.updated	2023-02-01T00:12:05Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	152
utslib.citation.issue	2

Abstract:

Flash-induced chlorophyll fluorescence relaxation is a powerful tool to monitor the reoxidation reactions of the reduced primary quinone acceptor, QA- by QB and the plastoquinone (PQ) pool, as well as the charge recombination reactions between the donor and acceptor side components of Photosystem II (PSII). Under certain conditions, when the PQ pool is highly reduced (e.g. in microaerobic conditions), a wave phenomenon appears in the fluorescence relaxation kinetics, which reflects the transient reoxidation and re-reduction of QA- by various electron transfer processes, which in cyanobacteria is mediated by NAD(P)H dehydrogenase (NDH-1). The wave phenomenon was also observed and assigned to the operation of type 2 NAD(P)H dehydrogenase (NDH-2) in the green alga Chlamydomonas reinhardtii under hydrogen-producing conditions, which required a long incubation of algae under sulphur deprivation (Krishna et al. J Exp Bot 70 (21):6321-6336, 2019). However, the conditions that induce the wave remained largely uncharacterized so far in microalgae. In this work, we investigated the wave phenomenon in Chlamydomonas reinhardtii under conditions that lead to a decrease of PSII activity by applying hydroxylamine treatment, which impacts the donor side of PSII in combination with a strongly reducing environment of the PQ pool (microaerobic conditions). A similar wave phenomenon could be induced by photoinhibitory conditions (illumination with strong light in the presence of the protein synthesis inhibitor lincomycin). These results indicate that the fluorescence wave phenomenon is activated in green algae when the PSII activity decreases relative to Photosystem I (PS I) activity and the PQ pool is strongly reduced. Therefore, the fluorescence wave could be used as a sensitive indicator of altered intersystem electron transfer processes, e.g. under stress conditions.

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http://hdl.handle.net/10453/165715