Measuring anion binding at biomembrane interfaces.

Wu, X; Wang, P; Lewis, W; Jiang, Y-B; Gale, PA

Measuring anion binding at biomembrane interfaces.

Wu, X Wang, P Lewis, W Jiang, Y-B Gale, PA

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Publisher:: Springer Nature
Publication Type:: Journal Article
Citation:: Nat Commun, 2022, 13, (1), pp. 4623
Issue Date:: 2022-08-08

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Field	Value	Language
dc.contributor.author	Wu, X
dc.contributor.author	Wang, P
dc.contributor.author	Lewis, W
dc.contributor.author	Jiang, Y-B
dc.contributor.author	Gale, PA
dc.date.accessioned	2023-02-07T23:53:17Z
dc.date.available	2022-07-29
dc.date.available	2023-02-07T23:53:17Z
dc.date.issued	2022-08-08
dc.identifier.citation	Nat Commun, 2022, 13, (1), pp. 4623
dc.identifier.issn	2041-1723
dc.identifier.issn	2041-1723
dc.identifier.uri	http://hdl.handle.net/10453/165981
dc.description.abstract	The quantification of anion binding by molecular receptors within lipid bilayers remains challenging. Here we measure anion binding in lipid bilayers by creating a fluorescent macrocycle featuring a strong sulfate affinity. We find the determinants of anion binding in lipid bilayers to be different from those expected that govern anion binding in solution. Charge-dense anions H2PO4- and Cl- that prevail in dimethyl sulfoxide fail to bind to the macrocycle in lipids. In stark contrast, ClO4- and I- that hardly bind in dimethyl sulfoxide show surprisingly significant affinities for the macrocycle in lipids. We reveal a lipid bilayer anion binding principle that depends on anion polarisability and bilayer penetration depth of complexes leading to unexpected advantages of charge-diffuse anions. These insights enhance our understanding of how biological systems select anions and guide the design of functional molecular systems operating at biomembrane interfaces.
dc.format	Electronic
dc.language	eng
dc.publisher	Springer Nature
dc.relation	http://purl.org/au-research/grants/arc/DP210100039
dc.relation.ispartof	Nat Commun
dc.relation.isbasedon	10.1038/s41467-022-32403-z
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Anions
dc.subject.mesh	Dimethyl Sulfoxide
dc.subject.mesh	Lipid Bilayers
dc.subject.mesh	Sulfates
dc.subject.mesh	Anions
dc.subject.mesh	Sulfates
dc.subject.mesh	Dimethyl Sulfoxide
dc.subject.mesh	Lipid Bilayers
dc.subject.mesh	Anions
dc.subject.mesh	Dimethyl Sulfoxide
dc.subject.mesh	Lipid Bilayers
dc.subject.mesh	Sulfates
dc.title	Measuring anion binding at biomembrane interfaces.
dc.type	Journal Article
utslib.citation.volume	13
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.date.updated	2023-02-07T23:53:10Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	13
utslib.citation.issue	1

Abstract:

The quantification of anion binding by molecular receptors within lipid bilayers remains challenging. Here we measure anion binding in lipid bilayers by creating a fluorescent macrocycle featuring a strong sulfate affinity. We find the determinants of anion binding in lipid bilayers to be different from those expected that govern anion binding in solution. Charge-dense anions H2PO4- and Cl- that prevail in dimethyl sulfoxide fail to bind to the macrocycle in lipids. In stark contrast, ClO4- and I- that hardly bind in dimethyl sulfoxide show surprisingly significant affinities for the macrocycle in lipids. We reveal a lipid bilayer anion binding principle that depends on anion polarisability and bilayer penetration depth of complexes leading to unexpected advantages of charge-diffuse anions. These insights enhance our understanding of how biological systems select anions and guide the design of functional molecular systems operating at biomembrane interfaces.

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http://hdl.handle.net/10453/165981