The Arabidopsis Thylakoid Chloride Channel ClCe Regulates ATP Availability for Light-harvesting Complex II Protein Phosphorylation

Herdean, A; Dukic, E; Gollan, P; Grebe, S; Paakkarinen, V; Aro, E-M; Spetea, C

The Arabidopsis Thylakoid Chloride Channel ClCe Regulates ATP Availability for Light-harvesting Complex II Protein Phosphorylation

Herdean, A

Dukic, E Gollan, P Grebe, S Paakkarinen, V Aro, E-M Spetea, C

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Publisher:: Frontiers Media
Publication Type:: Journal Article
Citation:: Frontiers in Plant Science, 2022, 13, (Women in Plant Physiology: 2022), pp. 1050355
Issue Date:: 2022-10-31

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Field	Value	Language
dc.contributor.author	Herdean, A https://orcid.org/0000-0003-2143-0213
dc.contributor.author	Dukic, E
dc.contributor.author	Gollan, P
dc.contributor.author	Grebe, S
dc.contributor.author	Paakkarinen, V
dc.contributor.author	Aro, E-M
dc.contributor.author	Spetea, C
dc.date.accessioned	2023-02-14T03:26:30Z
dc.date.available	2022-10-31
dc.date.available	2023-02-14T03:26:30Z
dc.date.issued	2022-10-31
dc.identifier.citation	Frontiers in Plant Science, 2022, 13, (Women in Plant Physiology: 2022), pp. 1050355
dc.identifier.issn	1664-462X
dc.identifier.issn	1664-462X
dc.identifier.uri	http://hdl.handle.net/10453/166104
dc.description.abstract	Coping with changes in light intensity is challenging for plants, but well-designed mechanisms allow them to acclimate to most unpredicted situations. The thylakoid K+/H+ antiporter KEA3 and the voltage-dependent Cl− channel VCCN1 play important roles in light acclimation by fine-tuning electron transport and photoprotection. Good evidence exists that the thylakoid Cl− channel ClCe is involved in the regulation of photosynthesis and state transitions in conditions of low light. However, a detailed mechanistic understanding of this effect is lacking. Here we report that the ClCe loss-of-function in Arabidopsis thaliana results in lower levels of phosphorylated light-harvesting complex II (LHCII) proteins as well as lower levels of the photosystem I-LHCII complexes relative to wild type (WT) in low light conditions. The phosphorylation of the photosystem II core D1/D2/CP43 proteins was less affected either in low or high light conditions. In low light conditions, the steady-state levels of ATP synthase conductivity and of the total proton flux available for ATP synthesis were lower in ClCe loss-of-function mutants, but comparable to WT at standard and high light intensity. As a long-term acclimation strategy, expression of the ClCe gene was upregulated in WT plants grown in light-limiting conditions, but not in WT plants grown in standard light even when exposed for up to 8 h to low light. Taken together, these results suggest a role of ClCe in the regulation of the ATP synthase activity which under low light conditions impacts LHCII protein phosphorylation and state transitions.
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	Frontiers Media
dc.relation.ispartof	Frontiers in Plant Science
dc.relation.isbasedon	10.3389/fpls.2022.1050355
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0607 Plant Biology
dc.title	The Arabidopsis Thylakoid Chloride Channel ClCe Regulates ATP Availability for Light-harvesting Complex II Protein Phosphorylation
dc.type	Journal Article
utslib.citation.volume	13
utslib.location.activity	Switzerland
utslib.for	0607 Plant Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2023-02-14T03:26:12Z
pubs.issue	Women in Plant Physiology: 2022
pubs.publication-status	Published online
pubs.volume	13
utslib.citation.issue	Women in Plant Physiology: 2022

Abstract:

Coping with changes in light intensity is challenging for plants, but well-designed mechanisms allow them to acclimate to most unpredicted situations. The thylakoid K+/H+ antiporter KEA3 and the voltage-dependent Cl− channel VCCN1 play important roles in light acclimation by fine-tuning electron transport and photoprotection. Good evidence exists that the thylakoid Cl− channel ClCe is involved in the regulation of photosynthesis and state transitions in conditions of low light. However, a detailed mechanistic understanding of this effect is lacking. Here we report that the ClCe loss-of-function in Arabidopsis thaliana results in lower levels of phosphorylated light-harvesting complex II (LHCII) proteins as well as lower levels of the photosystem I-LHCII complexes relative to wild type (WT) in low light conditions. The phosphorylation of the photosystem II core D1/D2/CP43 proteins was less affected either in low or high light conditions. In low light conditions, the steady-state levels of ATP synthase conductivity and of the total proton flux available for ATP synthesis were lower in ClCe loss-of-function mutants, but comparable to WT at standard and high light intensity. As a long-term acclimation strategy, expression of the ClCe gene was upregulated in WT plants grown in light-limiting conditions, but not in WT plants grown in standard light even when exposed for up to 8 h to low light. Taken together, these results suggest a role of ClCe in the regulation of the ATP synthase activity which under low light conditions impacts LHCII protein phosphorylation and state transitions.

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http://hdl.handle.net/10453/166104