Temperature mapping of non‑photochemical quenching in Chlorella vulgaris
- Publisher:
- Springer
- Publication Type:
- Journal Article
- Citation:
- Photosynthesis Research, 2022, 155, (2), pp. 191-202
- Issue Date:
- 2022-10-25
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Light intensity and temperature independently impact all parts of the photosynthetic machinery in plants and algae. Yet to
date, the vast majority of pulse amplitude modulated (PAM) chlorophyll a fluorescence measurements have been performed
at well-defined light intensities, but rarely at well-defined temperatures. In this work, we show that PAM measurements
performed at various temperatures produce vastly different results in the chlorophyte Chlorella vulgaris. Using a recently
developed Phenoplate technique to map quantum yield of Photosystem II (Y(II)) and non-photochemical quenching (NPQ)
as a function of temperature, we show that the fast-relaxing NPQ follows an inverse normal distribution with respect to temperature
and appears insensitive to previous temperature acclimation. The slow-relaxing or residual NPQ after 5 minutes of
dark recovery follows a normal distribution similar to Y(II) but with a peak in the higher temperature range. Surprisingly,
higher slow- and fast-relaxing NPQ values were observed in high-light relative to low-light acclimated cultures. Y(II) values
peaked at the adaptation temperature regardless of temperature or light acclimation. Our novel findings show the complete
temperature working spectrum of Y(II) and how excess energy quenching is managed across a wide range of temperatures in
the model microalgal species C. vulgaris. Finally, we draw attention to the fact that the effect of the temperature component
in PAM measurements has been wildly underestimated, and results from experiments at room temperature can be misleading.
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