Monitoring of SARS-CoV-2 in sewersheds with low COVID-19 cases using a passive sampling technique.

Li, J; Ahmed, W; Metcalfe, S; Smith, WJM; Tscharke, B; Lynch, P; Sherman, P; Vo, PHN; Kaserzon, SL; Simpson, SL; McCarthy, DT; Thomas, KV; Mueller, JF; Thai, P

Monitoring of SARS-CoV-2 in sewersheds with low COVID-19 cases using a passive sampling technique.

Li, J Ahmed, W Metcalfe, S Smith, WJM Tscharke, B Lynch, P Sherman, P Vo, PHN Kaserzon, SL Simpson, SL McCarthy, DT Thomas, KV Mueller, JF Thai, P

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Water Res, 2022, 218, pp. 118481
Issue Date:: 2022-06-30

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Field	Value	Language
dc.contributor.author	Li, J
dc.contributor.author	Ahmed, W
dc.contributor.author	Metcalfe, S
dc.contributor.author	Smith, WJM
dc.contributor.author	Tscharke, B
dc.contributor.author	Lynch, P
dc.contributor.author	Sherman, P
dc.contributor.author	Vo, PHN
dc.contributor.author	Kaserzon, SL
dc.contributor.author	Simpson, SL
dc.contributor.author	McCarthy, DT
dc.contributor.author	Thomas, KV
dc.contributor.author	Mueller, JF
dc.contributor.author	Thai, P
dc.date.accessioned	2023-03-28T03:31:23Z
dc.date.available	2022-04-18
dc.date.available	2023-03-28T03:31:23Z
dc.date.issued	2022-06-30
dc.identifier.citation	Water Res, 2022, 218, pp. 118481
dc.identifier.issn	0043-1354
dc.identifier.issn	1879-2448
dc.identifier.uri	http://hdl.handle.net/10453/168695
dc.description.abstract	Monitoring SARS-CoV-2 RNA in sewer systems, upstream of a wastewater treatment plant, is an effective approach for understanding potential COVID-19 transmission in communities with higher spatial resolutions. Passive sampling devices provide a practical solution for frequent sampling within sewer networks where the use of autosamplers is not feasible. Currently, the design of upstream sampling is impeded by limited understanding of the fate of SARS-CoV-2 RNA in sewers and the sensitivity of passive samplers for the number of infected individuals in a catchment. In this study, passive samplers containing electronegative membranes were applied for at least 24-h continuous sampling in sewer systems. When monitoring SARS-CoV-2 along a trunk sewer pipe, we found RNA signals decreased proportionally to increasing dilutions, with non-detects occurring at the end of pipe. The passive sampling membranes were able to detect SARS-CoV-2 shed by >2 COVID-19 infection cases in 10,000 people. Moreover, upstream monitoring in multiple sewersheds using passive samplers identified the emergence of SARS-CoV-2 in wastewater one week ahead of clinical reporting and reflected the spatiotemporal spread of a COVID-19 cluster within a city. This study provides important information to guide the development of wastewater surveillance strategies at catchment and subcatchment levels using different sampling techniques.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier
dc.relation.ispartof	Water Res
dc.relation.isbasedon	10.1016/j.watres.2022.118481
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Environmental Engineering
dc.subject.mesh	COVID-19
dc.subject.mesh	Humans
dc.subject.mesh	RNA, Viral
dc.subject.mesh	SARS-CoV-2
dc.subject.mesh	Wastewater
dc.subject.mesh	Wastewater-Based Epidemiological Monitoring
dc.subject.mesh	Humans
dc.subject.mesh	RNA, Viral
dc.subject.mesh	Wastewater-Based Epidemiological Monitoring
dc.subject.mesh	COVID-19
dc.subject.mesh	SARS-CoV-2
dc.subject.mesh	Wastewater
dc.subject.mesh	COVID-19
dc.subject.mesh	Humans
dc.subject.mesh	RNA, Viral
dc.subject.mesh	SARS-CoV-2
dc.subject.mesh	Wastewater
dc.subject.mesh	Wastewater-Based Epidemiological Monitoring
dc.title	Monitoring of SARS-CoV-2 in sewersheds with low COVID-19 cases using a passive sampling technique.
dc.type	Journal Article
utslib.citation.volume	218
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access	*
dc.date.updated	2023-03-28T03:31:03Z
pubs.publication-status	Published
pubs.volume	218

Abstract:

Monitoring SARS-CoV-2 RNA in sewer systems, upstream of a wastewater treatment plant, is an effective approach for understanding potential COVID-19 transmission in communities with higher spatial resolutions. Passive sampling devices provide a practical solution for frequent sampling within sewer networks where the use of autosamplers is not feasible. Currently, the design of upstream sampling is impeded by limited understanding of the fate of SARS-CoV-2 RNA in sewers and the sensitivity of passive samplers for the number of infected individuals in a catchment. In this study, passive samplers containing electronegative membranes were applied for at least 24-h continuous sampling in sewer systems. When monitoring SARS-CoV-2 along a trunk sewer pipe, we found RNA signals decreased proportionally to increasing dilutions, with non-detects occurring at the end of pipe. The passive sampling membranes were able to detect SARS-CoV-2 shed by >2 COVID-19 infection cases in 10,000 people. Moreover, upstream monitoring in multiple sewersheds using passive samplers identified the emergence of SARS-CoV-2 in wastewater one week ahead of clinical reporting and reflected the spatiotemporal spread of a COVID-19 cluster within a city. This study provides important information to guide the development of wastewater surveillance strategies at catchment and subcatchment levels using different sampling techniques.

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http://hdl.handle.net/10453/168695