A 3D bioprinted decellularized extracellular matrix/gelatin/quaternized chitosan scaffold assembling with poly(ionic liquid)s for skin tissue engineering.

Xu, J; Fang, H; Su, Y; Kang, Y; Xu, D; Cheng, YY; Nie, Y; Wang, H; Liu, T; Song, K

A 3D bioprinted decellularized extracellular matrix/gelatin/quaternized chitosan scaffold assembling with poly(ionic liquid)s for skin tissue engineering.

Xu, J Fang, H Su, Y Kang, Y Xu, D Cheng, YY Nie, Y Wang, H Liu, T Song, K

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Int J Biol Macromol, 2022, 220, pp. 1253-1266
Issue Date:: 2022-11-01

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Field	Value	Language
dc.contributor.author	Xu, J
dc.contributor.author	Fang, H
dc.contributor.author	Su, Y
dc.contributor.author	Kang, Y
dc.contributor.author	Xu, D
dc.contributor.author	Cheng, YY
dc.contributor.author	Nie, Y
dc.contributor.author	Wang, H
dc.contributor.author	Liu, T
dc.contributor.author	Song, K
dc.date.accessioned	2023-04-11T01:06:30Z
dc.date.available	2022-08-22
dc.date.available	2023-04-11T01:06:30Z
dc.date.issued	2022-11-01
dc.identifier.citation	Int J Biol Macromol, 2022, 220, pp. 1253-1266
dc.identifier.issn	0141-8130
dc.identifier.issn	1879-0003
dc.identifier.uri	http://hdl.handle.net/10453/169478
dc.description.abstract	Currently, a suitable bioink for 3D bioprinting and capable of mimicking the microenvironment of native skin and preventing bacterial infection remains a major challenge in skin tissue engineering. In this study, we prepared a tissue-specific extracellular matrix-based bioink, and dECM/Gel/QCS (dGQ) 3D scaffold assembling with poly(ionic liquid)s (PILs) (dGQP) was obtained by an extrusion 3D bioprinting technology and dynamic hydrogen bonding method. The morphologies, mechanical properties, porosity, hydrophilicity, biodegradation, hemostatic effect, antibacterial ability, and biocompatibility of the hybrid scaffolds were characterized and evaluated. Results showed that the rapid release (2 h) of PILs on the dGQP scaffold can quickly kill gram-negative (E. coli) and gram-positive (S. aureus) bacteria with almost 100 % antibacterial activity and maintained a stable sterile environment for a long time (7 d), which was superior to the dGQ scaffold. The hemostasis and hemolysis test showed that the dGQP scaffold had a good hemostatic effect and excellent hemocompatibility. In vitro cytocompatibility studies showed that although the cell growth on dGQP scaffold was slow in the early stage, the cells proliferated rapidly since day 4 and had high ECM secretion at day 7. Overall, this advanced dGQP scaffold has a considerable potential to be applied in skin tissue engineering.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier
dc.relation.ispartof	Int J Biol Macromol
dc.relation.isbasedon	10.1016/j.ijbiomac.2022.08.149
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0601 Biochemistry and Cell Biology
dc.subject.classification	Polymers
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Chitosan
dc.subject.mesh	Decellularized Extracellular Matrix
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Gelatin
dc.subject.mesh	Hemostatics
dc.subject.mesh	Ionic Liquids
dc.subject.mesh	Printing, Three-Dimensional
dc.subject.mesh	Staphylococcus aureus
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Staphylococcus aureus
dc.subject.mesh	Chitosan
dc.subject.mesh	Gelatin
dc.subject.mesh	Hemostatics
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Ionic Liquids
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Printing, Three-Dimensional
dc.subject.mesh	Decellularized Extracellular Matrix
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Chitosan
dc.subject.mesh	Decellularized Extracellular Matrix
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Gelatin
dc.subject.mesh	Hemostatics
dc.subject.mesh	Ionic Liquids
dc.subject.mesh	Printing, Three-Dimensional
dc.subject.mesh	Staphylococcus aureus
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Tissue Scaffolds
dc.title	A 3D bioprinted decellularized extracellular matrix/gelatin/quaternized chitosan scaffold assembling with poly(ionic liquid)s for skin tissue engineering.
dc.type	Journal Article
utslib.citation.volume	220
utslib.location.activity	Netherlands
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2023-04-11T01:06:03Z
pubs.publication-status	Published
pubs.volume	220

Abstract:

Currently, a suitable bioink for 3D bioprinting and capable of mimicking the microenvironment of native skin and preventing bacterial infection remains a major challenge in skin tissue engineering. In this study, we prepared a tissue-specific extracellular matrix-based bioink, and dECM/Gel/QCS (dGQ) 3D scaffold assembling with poly(ionic liquid)s (PILs) (dGQP) was obtained by an extrusion 3D bioprinting technology and dynamic hydrogen bonding method. The morphologies, mechanical properties, porosity, hydrophilicity, biodegradation, hemostatic effect, antibacterial ability, and biocompatibility of the hybrid scaffolds were characterized and evaluated. Results showed that the rapid release (2 h) of PILs on the dGQP scaffold can quickly kill gram-negative (E. coli) and gram-positive (S. aureus) bacteria with almost 100 % antibacterial activity and maintained a stable sterile environment for a long time (7 d), which was superior to the dGQ scaffold. The hemostasis and hemolysis test showed that the dGQP scaffold had a good hemostatic effect and excellent hemocompatibility. In vitro cytocompatibility studies showed that although the cell growth on dGQP scaffold was slow in the early stage, the cells proliferated rapidly since day 4 and had high ECM secretion at day 7. Overall, this advanced dGQP scaffold has a considerable potential to be applied in skin tissue engineering.

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http://hdl.handle.net/10453/169478