Metabolomic profiling of anthropogenically threatened Australian seagrass Zostera muelleri using one- and two-dimensional gas chromatography

Kuzhiumparambil, U; Kumar, M; Nizio, KD; Alonso, D; Gorst-Allman, P; Kelly, C; MacLeod, B; Forbes, S; Ralph, P

Metabolomic profiling of anthropogenically threatened Australian seagrass Zostera muelleri using one- and two-dimensional gas chromatography

Kuzhiumparambil, U Kumar, M Nizio, KD Alonso, D Gorst-Allman, P Kelly, C MacLeod, B Forbes, S Ralph, P

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Publisher:: Elsevier
Publication Type:: Chapter
Citation:: Applied Environmental Metabolomics: Community Insights and Guidance from the Field, 2022, pp. 135-151
Issue Date:: 2022-01-01

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Field	Value	Language
dc.contributor.author	Kuzhiumparambil, U
dc.contributor.author	Kumar, M
dc.contributor.author	Nizio, KD
dc.contributor.author	Alonso, D
dc.contributor.author	Gorst-Allman, P
dc.contributor.author	Kelly, C
dc.contributor.author	MacLeod, B
dc.contributor.author	Forbes, S
dc.contributor.author	Ralph, P https://orcid.org/0000-0002-3103-7346
dc.date.accessioned	2023-05-29T23:59:02Z
dc.date.available	2023-05-29T23:59:02Z
dc.date.issued	2022-01-01
dc.identifier.citation	Applied Environmental Metabolomics: Community Insights and Guidance from the Field, 2022, pp. 135-151
dc.identifier.isbn	9780128164617
dc.identifier.uri	http://hdl.handle.net/10453/170532
dc.description.abstract	The global decline of seagrass meadows due to sustained pressure from anthropogenic activities and the ongoing threat from climate change has weakened their capacity for supporting coastal productivity and fisheries habitats, while also increasing sediment erosion. The ongoing efforts to prevent seagrass decline require novel tools to monitor seagrass health and assess the effects of habitat management. Such monitoring tools require the use of sensitive indicators to assess the intensity of environmental stressors and to monitor the corresponding responses of seagrass. Environmental metabolomics has proven valuable in identifying such phenotypic traits of abiotic and biotic stress in plants. The identification of metabolite changes in seagrass linked to an environmental stress response may lead to the development of a molecular diagnostic tool that could be used to give an early warning of seagrass stress. Therefore, we explore the potential of one- and two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (1D and 2D GC-TOFMS) in Australia’s most threatened seagrass species—Zostera muelleri. Overall, GC×GC-TOFMS outperformed GC-TOFMS and offered a robust, comprehensive, and superior analytical sensitivity and resolution with a total of 156 metabolites compared to 93 identified in GC-TOFMS. Among these metabolites, > 50% were identified exclusively in GC×GC-TOFMS and include secondary metabolites of the phenylpropenoid class, phytohormones, and various sugar and amino acid derivatives. Therefore, GC×GC-TOFMS represents a comprehensive metabolomics platform for both discovery and targeted studies in seagrass that may aid diagnostic tool development for more targeted seagrass management.
dc.language	en
dc.publisher	Elsevier
dc.relation	http://purl.org/au-research/grants/arc/DE150100461
dc.relation.ispartof	Applied Environmental Metabolomics: Community Insights and Guidance from the Field
dc.relation.isbasedon	10.1016/B978-0-12-816460-0.00018-6
dc.rights	info:eu-repo/semantics/closedAccess
dc.title	Metabolomic profiling of anthropogenically threatened Australian seagrass Zostera muelleri using one- and two-dimensional gas chromatography
dc.type	Chapter
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access	*
dc.date.updated	2023-05-29T23:58:58Z
pubs.publication-status	Published

Abstract:

The global decline of seagrass meadows due to sustained pressure from anthropogenic activities and the ongoing threat from climate change has weakened their capacity for supporting coastal productivity and fisheries habitats, while also increasing sediment erosion. The ongoing efforts to prevent seagrass decline require novel tools to monitor seagrass health and assess the effects of habitat management. Such monitoring tools require the use of sensitive indicators to assess the intensity of environmental stressors and to monitor the corresponding responses of seagrass. Environmental metabolomics has proven valuable in identifying such phenotypic traits of abiotic and biotic stress in plants. The identification of metabolite changes in seagrass linked to an environmental stress response may lead to the development of a molecular diagnostic tool that could be used to give an early warning of seagrass stress. Therefore, we explore the potential of one- and two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (1D and 2D GC-TOFMS) in Australia’s most threatened seagrass species—Zostera muelleri. Overall, GC×GC-TOFMS outperformed GC-TOFMS and offered a robust, comprehensive, and superior analytical sensitivity and resolution with a total of 156 metabolites compared to 93 identified in GC-TOFMS. Among these metabolites, > 50% were identified exclusively in GC×GC-TOFMS and include secondary metabolites of the phenylpropenoid class, phytohormones, and various sugar and amino acid derivatives. Therefore, GC×GC-TOFMS represents a comprehensive metabolomics platform for both discovery and targeted studies in seagrass that may aid diagnostic tool development for more targeted seagrass management.

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http://hdl.handle.net/10453/170532