Two-photon MINFLUX with doubled localization precision

Zhao, K; Xu, X; Ren, W; Jin, D; Xi, P

Two-photon MINFLUX with doubled localization precision

Zhao, K Xu, X Ren, W Jin, D

Xi, P

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Publisher:: Springer Nature
Publication Type:: Journal Article
Citation:: eLight, 2022, 2, (1), pp. 5
Issue Date:: 2022-12-01

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Field	Value	Language
dc.contributor.author	Zhao, K
dc.contributor.author	Xu, X
dc.contributor.author	Ren, W
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.contributor.author	Xi, P
dc.date.accessioned	2023-07-18T23:06:48Z
dc.date.available	2023-07-18T23:06:48Z
dc.date.issued	2022-12-01
dc.identifier.citation	eLight, 2022, 2, (1), pp. 5
dc.identifier.issn	2097-1710
dc.identifier.issn	2662-8643
dc.identifier.uri	http://hdl.handle.net/10453/171566
dc.description.abstract	Achieving localization with molecular precision has been of great interest for extending fluorescence microscopy to nanoscopy. MINFLUX pioneers this transition through point spread function (PSF) engineering, yet its performance is primarily limited by the signal-to-background ratio. Here we demonstrate theoretically that two-photon MINFLUX (2p-MINFLUX) could double its localization precision through PSF engineering by nonlinear effect. Cramér-Rao Bound (CRB) is studied as the maximum localization precision, and CRB of two-photon MINFLUX is halved compared to single-photon MINFLUX (1p-MINFLUX) in all three dimensions. Meanwhile, in order to achieve same localization precision with 1p-MINFLUX, 2p-MINFLUX requires only 1/4 of fluorescence photons. Exploiting simultaneous two-photon excitation of multiple fluorophore species, 2p-MINFLUX may have the potential for registration-free nanoscopy and multicolor tracking.
dc.language	en
dc.publisher	Springer Nature
dc.relation	National Natural Science Foundation of China61729501
dc.relation.ispartof	eLight
dc.relation.isbasedon	10.1186/s43593-021-00011-x
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Two-photon MINFLUX with doubled localization precision
dc.type	Journal Article
utslib.citation.volume	2
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access	*
dc.date.updated	2023-07-18T23:06:47Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	2
utslib.citation.issue	1

Abstract:

Achieving localization with molecular precision has been of great interest for extending fluorescence microscopy to nanoscopy. MINFLUX pioneers this transition through point spread function (PSF) engineering, yet its performance is primarily limited by the signal-to-background ratio. Here we demonstrate theoretically that two-photon MINFLUX (2p-MINFLUX) could double its localization precision through PSF engineering by nonlinear effect. Cramér-Rao Bound (CRB) is studied as the maximum localization precision, and CRB of two-photon MINFLUX is halved compared to single-photon MINFLUX (1p-MINFLUX) in all three dimensions. Meanwhile, in order to achieve same localization precision with 1p-MINFLUX, 2p-MINFLUX requires only 1/4 of fluorescence photons. Exploiting simultaneous two-photon excitation of multiple fluorophore species, 2p-MINFLUX may have the potential for registration-free nanoscopy and multicolor tracking.

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http://hdl.handle.net/10453/171566