Disassembly of the divisome in Escherichia coli: evidence that FtsZ dissociates before compartmentalization.

Söderström, B; Skoog, K; Blom, H; Weiss, DS; von Heijne, G; Daley, DO

Disassembly of the divisome in Escherichia coli: evidence that FtsZ dissociates before compartmentalization.

Söderström, B Skoog, K Blom, H Weiss, DS von Heijne, G Daley, DO

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Publisher:: WILEY-BLACKWELL
Publication Type:: Journal Article
Citation:: Mol Microbiol, 2014, 92, (1), pp. 1-9
Issue Date:: 2014-04

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Field	Value	Language
dc.contributor.author	Söderström, B
dc.contributor.author	Skoog, K
dc.contributor.author	Blom, H
dc.contributor.author	Weiss, DS
dc.contributor.author	von Heijne, G
dc.contributor.author	Daley, DO
dc.date.accessioned	2023-08-02T22:55:46Z
dc.date.available	2014-01-28
dc.date.available	2023-08-02T22:55:46Z
dc.date.issued	2014-04
dc.identifier.citation	Mol Microbiol, 2014, 92, (1), pp. 1-9
dc.identifier.issn	0950-382X
dc.identifier.issn	1365-2958
dc.identifier.uri	http://hdl.handle.net/10453/171705
dc.description.abstract	In most bacteria cell division is mediated by a protein super-complex called the divisome that co-ordinates the constriction and scission of the cell envelope. FtsZ is the first of the divisome proteins to accumulate at the division site and is widely thought to function as a force generator that constricts the cell envelope. In this study we have used a combination of confocal fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) to determine if divisome proteins are present at the septum at the time of cytoplasmic compartmentalization in Escherichia coli. Our data suggest that many are, but that FtsZ and ZapA disassemble before the cytoplasm is sealed by constriction of the inner membrane. This observation implies that FtsZ cannot be a force generator during the final stage(s) of envelope constriction in E. coli.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY-BLACKWELL
dc.relation.ispartof	Mol Microbiol
dc.relation.isbasedon	10.1111/mmi.12534
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	06 Biological Sciences, 07 Agricultural and Veterinary Sciences, 11 Medical and Health Sciences
dc.subject.classification	Microbiology
dc.subject.classification	31 Biological sciences
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Carrier Proteins
dc.subject.mesh	Cell Division
dc.subject.mesh	Cytoplasm
dc.subject.mesh	Cytoskeletal Proteins
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Escherichia coli Proteins
dc.subject.mesh	Fluorescence Recovery After Photobleaching
dc.subject.mesh	Microscopy, Confocal
dc.subject.mesh	Recombinant Fusion Proteins
dc.subject.mesh	Cytoplasm
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Escherichia coli Proteins
dc.subject.mesh	Carrier Proteins
dc.subject.mesh	Cytoskeletal Proteins
dc.subject.mesh	Recombinant Fusion Proteins
dc.subject.mesh	Microscopy, Confocal
dc.subject.mesh	Fluorescence Recovery After Photobleaching
dc.subject.mesh	Cell Division
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Carrier Proteins
dc.subject.mesh	Cell Division
dc.subject.mesh	Cytoplasm
dc.subject.mesh	Cytoskeletal Proteins
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Escherichia coli Proteins
dc.subject.mesh	Fluorescence Recovery After Photobleaching
dc.subject.mesh	Microscopy, Confocal
dc.subject.mesh	Recombinant Fusion Proteins
dc.title	Disassembly of the divisome in Escherichia coli: evidence that FtsZ dissociates before compartmentalization.
dc.type	Journal Article
utslib.citation.volume	92
utslib.location.activity	England
utslib.for	06 Biological Sciences
utslib.for	07 Agricultural and Veterinary Sciences
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - AIMI - Australian Institute for Microbiology & Infection
utslib.copyright.status	closed_access	*
dc.date.updated	2023-08-02T22:55:42Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	92
utslib.citation.issue	1

Abstract:

In most bacteria cell division is mediated by a protein super-complex called the divisome that co-ordinates the constriction and scission of the cell envelope. FtsZ is the first of the divisome proteins to accumulate at the division site and is widely thought to function as a force generator that constricts the cell envelope. In this study we have used a combination of confocal fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) to determine if divisome proteins are present at the septum at the time of cytoplasmic compartmentalization in Escherichia coli. Our data suggest that many are, but that FtsZ and ZapA disassemble before the cytoplasm is sealed by constriction of the inner membrane. This observation implies that FtsZ cannot be a force generator during the final stage(s) of envelope constriction in E. coli.

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http://hdl.handle.net/10453/171705