Third-Hand Exposure to E-Cigarette Vapour Induces Pulmonary Effects in Mice

Thorpe, AE; Donovan, C; Kim, RY; Vindin, HJ; Zakarya, R; Miyai, H; Chan, YL; van Reyk, D; Chen, H; Oliver, BG

Third-Hand Exposure to E-Cigarette Vapour Induces Pulmonary Effects in Mice

Thorpe, AE Donovan, C Kim, RY Vindin, HJ Zakarya, R Miyai, H Chan, YL van Reyk, D Chen, H

Oliver, BG

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Publisher:: MDPI AG
Publication Type:: Journal Article
Citation:: Toxics, 11, (9), pp. 749-749

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Field	Value	Language
dc.contributor.author	Thorpe, AE
dc.contributor.author	Donovan, C
dc.contributor.author	Kim, RY
dc.contributor.author	Vindin, HJ
dc.contributor.author	Zakarya, R
dc.contributor.author	Miyai, H
dc.contributor.author	Chan, YL
dc.contributor.author	van Reyk, D
dc.contributor.author	Chen, H https://orcid.org/0000-0001-6883-3752
dc.contributor.author	Oliver, BG
dc.date.accessioned	2023-09-07T10:14:18Z
dc.date.available	2023-09-07T10:14:18Z
dc.identifier.citation	Toxics, 11, (9), pp. 749-749
dc.identifier.issn	2305-6304
dc.identifier.uri	http://hdl.handle.net/10453/171978
dc.description.abstract	<jats:p>In the last decade, e-cigarette usage has increased, with an estimated 82 million e-cigarette users globally. This is, in part, due to the common opinion that they are “healthier” than tobacco cigarettes or simply “water vapour”. Third-hand e-vapour exposure is the chemical residue left behind from e-cigarette aerosols, which is of concern due to its invisible nature, especially among young children. However, there is limited information surrounding third-hand e-vapour exposure. This study aimed to investigate the pulmonary effects of sub-chronic third-hand e-vapour exposure in a murine model. BALB/c mice (4 weeks of age) were exposed to a towel containing nicotine free (0 mg) e-vapour, nicotine (18 mg) e-vapour, or no e-vapour (sham) and replaced daily for 4 weeks. At the endpoint, lung function was assessed, and bronchoalveolar lavage fluid and lungs were collected to measure inflammation and fibrosis. Mice exposed to third-hand e-vapour without nicotine had alveolar enlargement compared to sham exposed controls. Mice exposed to third-hand e-vapour with nicotine had reduced bronchial responsiveness to provocation, increased epithelial thickening in large airways, increased epithelial layers in small airways, alveolar enlargement, and increased small airway collagen deposition, compared to sham exposed controls. In conclusion, our study shows that third-hand e-vapour exposure, particularly in the presence of nicotine, negatively affects the lung health of mice and highlights the need for greater public awareness surrounding the dangers of third-hand exposure to e-cigarette vapour.</jats:p>
dc.language	en
dc.publisher	MDPI AG
dc.relation	http://purl.org/au-research/grants/nhmrc/GNT1158186
dc.relation.ispartof	Toxics
dc.relation.isbasedon	10.3390/toxics11090749
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Third-Hand Exposure to E-Cigarette Vapour Induces Pulmonary Effects in Mice
dc.type	Journal Article
utslib.citation.volume	11
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2023-09-07T10:14:08Z
pubs.issue	9
pubs.publication-status	Published online
pubs.volume	11
utslib.citation.issue	9

Abstract:

In the last decade, e-cigarette usage has increased, with an estimated 82 million e-cigarette users globally. This is, in part, due to the common opinion that they are “healthier” than tobacco cigarettes or simply “water vapour”. Third-hand e-vapour exposure is the chemical residue left behind from e-cigarette aerosols, which is of concern due to its invisible nature, especially among young children. However, there is limited information surrounding third-hand e-vapour exposure. This study aimed to investigate the pulmonary effects of sub-chronic third-hand e-vapour exposure in a murine model. BALB/c mice (4 weeks of age) were exposed to a towel containing nicotine free (0 mg) e-vapour, nicotine (18 mg) e-vapour, or no e-vapour (sham) and replaced daily for 4 weeks. At the endpoint, lung function was assessed, and bronchoalveolar lavage fluid and lungs were collected to measure inflammation and fibrosis. Mice exposed to third-hand e-vapour without nicotine had alveolar enlargement compared to sham exposed controls. Mice exposed to third-hand e-vapour with nicotine had reduced bronchial responsiveness to provocation, increased epithelial thickening in large airways, increased epithelial layers in small airways, alveolar enlargement, and increased small airway collagen deposition, compared to sham exposed controls. In conclusion, our study shows that third-hand e-vapour exposure, particularly in the presence of nicotine, negatively affects the lung health of mice and highlights the need for greater public awareness surrounding the dangers of third-hand exposure to e-cigarette vapour.

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http://hdl.handle.net/10453/171978