Genomic profiling of Pasteurella multocida isolated from feedlot cases of bovine respiratory disease.

Alhamami, T; Roy Chowdhury, P; Venter, H; Veltman, T; Truswell, A; Abraham, S; Sapula, SA; Carr, M; Djordjevic, SP; Trott, DJ

Genomic profiling of Pasteurella multocida isolated from feedlot cases of bovine respiratory disease.

Alhamami, T Roy Chowdhury, P Venter, H Veltman, T Truswell, A Abraham, S Sapula, SA Carr, M Djordjevic, SP Trott, DJ

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Vet Microbiol, 2023, 283, pp. 109773
Issue Date:: 2023-08

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Field	Value	Language
dc.contributor.author	Alhamami, T
dc.contributor.author	Roy Chowdhury, P
dc.contributor.author	Venter, H
dc.contributor.author	Veltman, T
dc.contributor.author	Truswell, A
dc.contributor.author	Abraham, S
dc.contributor.author	Sapula, SA
dc.contributor.author	Carr, M
dc.contributor.author	Djordjevic, SP
dc.contributor.author	Trott, DJ
dc.date.accessioned	2023-09-29T04:24:44Z
dc.date.available	2023-05-06
dc.date.available	2023-09-29T04:24:44Z
dc.date.issued	2023-08
dc.identifier.citation	Vet Microbiol, 2023, 283, pp. 109773
dc.identifier.issn	0378-1135
dc.identifier.issn	1873-2542
dc.identifier.uri	http://hdl.handle.net/10453/172380
dc.description.abstract	Pasteurella multocida causes a range of diseases in many host species throughout the world, including bovine respiratory disease (BRD) which is predominantly seen in feedlot cattle. This study assessed genetic diversity among 139 P. multocida isolates obtained from post-mortem lung swabs of BRD-affected feedlot cattle in four Australian states: New South Wales, Queensland, South Australia, and Victoria during 2014-2019. Whole-genome sequencing (WGS) was used to determine capsular serogroup, lipopolysaccharide genotypes, multi-locus sequence types and phylogenetic relationships. Two capsular types (A and D), with most isolates (132/139; 95%) belonging to type A; and three lipopolysaccharide (LPS) genotypes were identified (L1 [6/139; 4.3%], L3 [124/139; 89.2%] and L6 [9/139; 6.4%)]). Multi-locus sequence types (STs) ST9, ST13, ST17, ST20, ST36, ST50, ST58, ST79, ST124, ST125, ST132, ST167, ST185, ST327, ST394, and three novel STs [ST396, ST397, and ST398] were identified, with ST394 (59/139; 42.4%) and ST79 (44/139; 32%) the most prevalent in all four states. Isolates displaying phenotypic resistance to single, dual or multiple antibiotics (macrolide, tetracycline and aminopenicillins) were predominantly ST394 (23/139; 17%). Laterally mobile elements identified in the resistant ST394 isolates included small plasmids, encoding macrolide and/or tetracycline resistance, distributed in all states; and chromosomally located integrative conjugative elements (ICEs) (4 ST394 and 1 ST125) from the same Queensland feedlot. This study highlights the genomic diversity, epidemiological relationships and AMR associations in bovine P. multocida isolates from Australia and provides insight into the unique ST prevalence compared to other major beef-producing countries.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier
dc.relation.ispartof	Vet Microbiol
dc.relation.isbasedon	10.1016/j.vetmic.2023.109773
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0605 Microbiology, 0707 Veterinary Sciences
dc.subject.classification	Veterinary Sciences
dc.subject.classification	3009 Veterinary sciences
dc.subject.classification	3107 Microbiology
dc.subject.mesh	Cattle
dc.subject.mesh	Animals
dc.subject.mesh	Pasteurella multocida
dc.subject.mesh	Lipopolysaccharides
dc.subject.mesh	Phylogeny
dc.subject.mesh	Cattle Diseases
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Respiratory Tract Diseases
dc.subject.mesh	Genomics
dc.subject.mesh	Macrolides
dc.subject.mesh	Victoria
dc.subject.mesh	Pasteurella Infections
dc.subject.mesh	Animals
dc.subject.mesh	Cattle
dc.subject.mesh	Pasteurella multocida
dc.subject.mesh	Pasteurella Infections
dc.subject.mesh	Respiratory Tract Diseases
dc.subject.mesh	Cattle Diseases
dc.subject.mesh	Macrolides
dc.subject.mesh	Lipopolysaccharides
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Genomics
dc.subject.mesh	Phylogeny
dc.subject.mesh	Victoria
dc.subject.mesh	Cattle
dc.subject.mesh	Animals
dc.subject.mesh	Pasteurella multocida
dc.subject.mesh	Lipopolysaccharides
dc.subject.mesh	Phylogeny
dc.subject.mesh	Cattle Diseases
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Respiratory Tract Diseases
dc.subject.mesh	Genomics
dc.subject.mesh	Macrolides
dc.subject.mesh	Victoria
dc.subject.mesh	Pasteurella Infections
dc.title	Genomic profiling of Pasteurella multocida isolated from feedlot cases of bovine respiratory disease.
dc.type	Journal Article
utslib.citation.volume	283
utslib.location.activity	Netherlands
utslib.for	0605 Microbiology
utslib.for	0707 Veterinary Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - AIMI - Australian Institute for Microbiology & Infection
utslib.copyright.status	closed_access	*
dc.date.updated	2023-09-29T04:24:42Z
pubs.publication-status	Published
pubs.volume	283

Abstract:

Pasteurella multocida causes a range of diseases in many host species throughout the world, including bovine respiratory disease (BRD) which is predominantly seen in feedlot cattle. This study assessed genetic diversity among 139 P. multocida isolates obtained from post-mortem lung swabs of BRD-affected feedlot cattle in four Australian states: New South Wales, Queensland, South Australia, and Victoria during 2014-2019. Whole-genome sequencing (WGS) was used to determine capsular serogroup, lipopolysaccharide genotypes, multi-locus sequence types and phylogenetic relationships. Two capsular types (A and D), with most isolates (132/139; 95%) belonging to type A; and three lipopolysaccharide (LPS) genotypes were identified (L1 [6/139; 4.3%], L3 [124/139; 89.2%] and L6 [9/139; 6.4%)]). Multi-locus sequence types (STs) ST9, ST13, ST17, ST20, ST36, ST50, ST58, ST79, ST124, ST125, ST132, ST167, ST185, ST327, ST394, and three novel STs [ST396, ST397, and ST398] were identified, with ST394 (59/139; 42.4%) and ST79 (44/139; 32%) the most prevalent in all four states. Isolates displaying phenotypic resistance to single, dual or multiple antibiotics (macrolide, tetracycline and aminopenicillins) were predominantly ST394 (23/139; 17%). Laterally mobile elements identified in the resistant ST394 isolates included small plasmids, encoding macrolide and/or tetracycline resistance, distributed in all states; and chromosomally located integrative conjugative elements (ICEs) (4 ST394 and 1 ST125) from the same Queensland feedlot. This study highlights the genomic diversity, epidemiological relationships and AMR associations in bovine P. multocida isolates from Australia and provides insight into the unique ST prevalence compared to other major beef-producing countries.

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http://hdl.handle.net/10453/172380