Genomic profiling of Pasteurella multocida isolated from feedlot cases of bovine respiratory disease.
Alhamami, T
Roy Chowdhury, P
Venter, H
Veltman, T
Truswell, A
Abraham, S
Sapula, SA
Carr, M
Djordjevic, SP
Trott, DJ
- Publisher:
- Elsevier
- Publication Type:
- Journal Article
- Citation:
- Vet Microbiol, 2023, 283, pp. 109773
- Issue Date:
- 2023-08
Closed Access
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1-s2.0-S0378113523001256-main.pdf | Accepted version | 3.2 MB | Adobe PDF |
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Alhamami, T | |
dc.contributor.author | Roy Chowdhury, P | |
dc.contributor.author | Venter, H | |
dc.contributor.author | Veltman, T | |
dc.contributor.author | Truswell, A | |
dc.contributor.author | Abraham, S | |
dc.contributor.author | Sapula, SA | |
dc.contributor.author | Carr, M | |
dc.contributor.author | Djordjevic, SP | |
dc.contributor.author | Trott, DJ | |
dc.date.accessioned | 2023-09-29T04:24:44Z | |
dc.date.available | 2023-05-06 | |
dc.date.available | 2023-09-29T04:24:44Z | |
dc.date.issued | 2023-08 | |
dc.identifier.citation | Vet Microbiol, 2023, 283, pp. 109773 | |
dc.identifier.issn | 0378-1135 | |
dc.identifier.issn | 1873-2542 | |
dc.identifier.uri | http://hdl.handle.net/10453/172380 | |
dc.description.abstract | Pasteurella multocida causes a range of diseases in many host species throughout the world, including bovine respiratory disease (BRD) which is predominantly seen in feedlot cattle. This study assessed genetic diversity among 139 P. multocida isolates obtained from post-mortem lung swabs of BRD-affected feedlot cattle in four Australian states: New South Wales, Queensland, South Australia, and Victoria during 2014-2019. Whole-genome sequencing (WGS) was used to determine capsular serogroup, lipopolysaccharide genotypes, multi-locus sequence types and phylogenetic relationships. Two capsular types (A and D), with most isolates (132/139; 95%) belonging to type A; and three lipopolysaccharide (LPS) genotypes were identified (L1 [6/139; 4.3%], L3 [124/139; 89.2%] and L6 [9/139; 6.4%)]). Multi-locus sequence types (STs) ST9, ST13, ST17, ST20, ST36, ST50, ST58, ST79, ST124, ST125, ST132, ST167, ST185, ST327, ST394, and three novel STs [ST396, ST397, and ST398] were identified, with ST394 (59/139; 42.4%) and ST79 (44/139; 32%) the most prevalent in all four states. Isolates displaying phenotypic resistance to single, dual or multiple antibiotics (macrolide, tetracycline and aminopenicillins) were predominantly ST394 (23/139; 17%). Laterally mobile elements identified in the resistant ST394 isolates included small plasmids, encoding macrolide and/or tetracycline resistance, distributed in all states; and chromosomally located integrative conjugative elements (ICEs) (4 ST394 and 1 ST125) from the same Queensland feedlot. This study highlights the genomic diversity, epidemiological relationships and AMR associations in bovine P. multocida isolates from Australia and provides insight into the unique ST prevalence compared to other major beef-producing countries. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | Elsevier | |
dc.relation.ispartof | Vet Microbiol | |
dc.relation.isbasedon | 10.1016/j.vetmic.2023.109773 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | 0605 Microbiology, 0707 Veterinary Sciences | |
dc.subject.classification | Veterinary Sciences | |
dc.subject.classification | 3009 Veterinary sciences | |
dc.subject.classification | 3107 Microbiology | |
dc.subject.mesh | Cattle | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Pasteurella multocida | |
dc.subject.mesh | Lipopolysaccharides | |
dc.subject.mesh | Phylogeny | |
dc.subject.mesh | Cattle Diseases | |
dc.subject.mesh | Anti-Bacterial Agents | |
dc.subject.mesh | Respiratory Tract Diseases | |
dc.subject.mesh | Genomics | |
dc.subject.mesh | Macrolides | |
dc.subject.mesh | Victoria | |
dc.subject.mesh | Pasteurella Infections | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Cattle | |
dc.subject.mesh | Pasteurella multocida | |
dc.subject.mesh | Pasteurella Infections | |
dc.subject.mesh | Respiratory Tract Diseases | |
dc.subject.mesh | Cattle Diseases | |
dc.subject.mesh | Macrolides | |
dc.subject.mesh | Lipopolysaccharides | |
dc.subject.mesh | Anti-Bacterial Agents | |
dc.subject.mesh | Genomics | |
dc.subject.mesh | Phylogeny | |
dc.subject.mesh | Victoria | |
dc.subject.mesh | Cattle | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Pasteurella multocida | |
dc.subject.mesh | Lipopolysaccharides | |
dc.subject.mesh | Phylogeny | |
dc.subject.mesh | Cattle Diseases | |
dc.subject.mesh | Anti-Bacterial Agents | |
dc.subject.mesh | Respiratory Tract Diseases | |
dc.subject.mesh | Genomics | |
dc.subject.mesh | Macrolides | |
dc.subject.mesh | Victoria | |
dc.subject.mesh | Pasteurella Infections | |
dc.title | Genomic profiling of Pasteurella multocida isolated from feedlot cases of bovine respiratory disease. | |
dc.type | Journal Article | |
utslib.citation.volume | 283 | |
utslib.location.activity | Netherlands | |
utslib.for | 0605 Microbiology | |
utslib.for | 0707 Veterinary Sciences | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Strength - AIMI - Australian Institute for Microbiology & Infection | |
utslib.copyright.status | closed_access | * |
dc.date.updated | 2023-09-29T04:24:42Z | |
pubs.publication-status | Published | |
pubs.volume | 283 |
Abstract:
Pasteurella multocida causes a range of diseases in many host species throughout the world, including bovine respiratory disease (BRD) which is predominantly seen in feedlot cattle. This study assessed genetic diversity among 139 P. multocida isolates obtained from post-mortem lung swabs of BRD-affected feedlot cattle in four Australian states: New South Wales, Queensland, South Australia, and Victoria during 2014-2019. Whole-genome sequencing (WGS) was used to determine capsular serogroup, lipopolysaccharide genotypes, multi-locus sequence types and phylogenetic relationships. Two capsular types (A and D), with most isolates (132/139; 95%) belonging to type A; and three lipopolysaccharide (LPS) genotypes were identified (L1 [6/139; 4.3%], L3 [124/139; 89.2%] and L6 [9/139; 6.4%)]). Multi-locus sequence types (STs) ST9, ST13, ST17, ST20, ST36, ST50, ST58, ST79, ST124, ST125, ST132, ST167, ST185, ST327, ST394, and three novel STs [ST396, ST397, and ST398] were identified, with ST394 (59/139; 42.4%) and ST79 (44/139; 32%) the most prevalent in all four states. Isolates displaying phenotypic resistance to single, dual or multiple antibiotics (macrolide, tetracycline and aminopenicillins) were predominantly ST394 (23/139; 17%). Laterally mobile elements identified in the resistant ST394 isolates included small plasmids, encoding macrolide and/or tetracycline resistance, distributed in all states; and chromosomally located integrative conjugative elements (ICEs) (4 ST394 and 1 ST125) from the same Queensland feedlot. This study highlights the genomic diversity, epidemiological relationships and AMR associations in bovine P. multocida isolates from Australia and provides insight into the unique ST prevalence compared to other major beef-producing countries.
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