Differential gene expression in a subpopulation of Phaeodactylum tricornutum with enhanced growth and carotenoid production after FACS-mediated selection

Macdonald Miller, S; Herdean, A; Gupta, V; Signal, B; Abbriano, RM; Ralph, PJ; Pernice, M

Differential gene expression in a subpopulation of Phaeodactylum tricornutum with enhanced growth and carotenoid production after FACS-mediated selection

Macdonald Miller, S Herdean, A

Gupta, V Signal, B Abbriano, RM Ralph, PJ Pernice, M

Permalink

Publisher:: Springer Nature
Publication Type:: Journal Article
Citation:: Journal of Applied Phycology, 2023, pp. 1-11
Issue Date:: 2023-01-01

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published versionAdobe PDF (1.81 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Macdonald Miller, S
dc.contributor.author	Herdean, A https://orcid.org/0000-0003-2143-0213
dc.contributor.author	Gupta, V
dc.contributor.author	Signal, B
dc.contributor.author	Abbriano, RM
dc.contributor.author	Ralph, PJ
dc.contributor.author	Pernice, M
dc.date.accessioned	2023-11-15T23:27:17Z
dc.date.available	2023-11-15T23:27:17Z
dc.date.issued	2023-01-01
dc.identifier.citation	Journal of Applied Phycology, 2023, pp. 1-11
dc.identifier.issn	0921-8971
dc.identifier.issn	1573-5176
dc.identifier.uri	http://hdl.handle.net/10453/173431
dc.description.abstract	Fluorescence-Activated Cell Sorting (FACS) is a powerful method with many applications in microalgal research, especially for screening and selection of cells with improved phenotypes. However, the technology requires review of gene expression changes responsible for enhanced phenotypes in sorted populations. Phaeodactylum tricornutum cells were sorted using FACS with excitation/emission parameters targeted to favouring the industrially-relevant carotenoid fucoxanthin. The resulting cultures showed significantly higher growth rate (1.10 ×), biomass (1.30 ×), chlorophyll a levels (1.22 ×) and fucoxanthin content (1.28 ×) relative to the wild-type strain. RNA-seq was used to elucidate the underlying molecular-level regulatory changes associated with these traits and represents the first study do so on FACS-sorted microalgal cultures. Transcriptome analysis corroborated evidence of increased chlorophyll a and fucoxanthin, showing enrichment for the genes/pathways for tetrapyrrole biosynthesis and for suites of genes directly related to photosynthesis. Only three genes were upregulated in the MEP (non-mevalonate) pathway to carotenoid biosynthesis pathway, suggesting either a strong influence of IDI, CRTISO5 and ZEP1 on fucoxanthin biosynthesis or a post-transcriptional or post-translational mechanism for the observed increase in fucoxanthin content.
dc.language	en
dc.publisher	Springer Nature
dc.relation	V2 Food Pty Ltd
dc.relation	V2 Food Pty Ltd
dc.relation	Department of Industry, Innovation and ScienceICG001925
dc.relation	University of Technology Sydney
dc.relation.ispartof	Journal of Applied Phycology
dc.relation.isbasedon	10.1007/s10811-023-03102-z
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0607 Plant Biology, 0704 Fisheries Sciences, 1002 Environmental Biotechnology
dc.subject.classification	Marine Biology & Hydrobiology
dc.subject.classification	3005 Fisheries sciences
dc.subject.classification	3108 Plant biology
dc.title	Differential gene expression in a subpopulation of Phaeodactylum tricornutum with enhanced growth and carotenoid production after FACS-mediated selection
dc.type	Journal Article
utslib.for	0607 Plant Biology
utslib.for	0704 Fisheries Sciences
utslib.for	1002 Environmental Biotechnology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access	*
dc.date.updated	2023-11-15T23:27:16Z
pubs.publication-status	Published

Abstract:

Fluorescence-Activated Cell Sorting (FACS) is a powerful method with many applications in microalgal research, especially for screening and selection of cells with improved phenotypes. However, the technology requires review of gene expression changes responsible for enhanced phenotypes in sorted populations. Phaeodactylum tricornutum cells were sorted using FACS with excitation/emission parameters targeted to favouring the industrially-relevant carotenoid fucoxanthin. The resulting cultures showed significantly higher growth rate (1.10 ×), biomass (1.30 ×), chlorophyll a levels (1.22 ×) and fucoxanthin content (1.28 ×) relative to the wild-type strain. RNA-seq was used to elucidate the underlying molecular-level regulatory changes associated with these traits and represents the first study do so on FACS-sorted microalgal cultures. Transcriptome analysis corroborated evidence of increased chlorophyll a and fucoxanthin, showing enrichment for the genes/pathways for tetrapyrrole biosynthesis and for suites of genes directly related to photosynthesis. Only three genes were upregulated in the MEP (non-mevalonate) pathway to carotenoid biosynthesis pathway, suggesting either a strong influence of IDI, CRTISO5 and ZEP1 on fucoxanthin biosynthesis or a post-transcriptional or post-translational mechanism for the observed increase in fucoxanthin content.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/173431