Quantitative and qualitative assessment of DNA recovered from human skeletal remains

Ward, J; Watherston, J

Quantitative and qualitative assessment of DNA recovered from human skeletal remains

Ward, J

Watherston, J

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Publisher:: Elsevier
Publication Type:: Chapter
Citation:: Forensic Genetic Approaches for Identification of Human Skeletal Remains: Challenges, Best Practices, and Emerging Technologies, 2023, pp. 137-163
Issue Date:: 2023-01-01

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Field	Value	Language
dc.contributor.author	Ward, J https://orcid.org/0000-0001-9359-9806
dc.contributor.author	Watherston, J
dc.date.accessioned	2023-11-23T23:51:25Z
dc.date.available	2023-11-23T23:51:25Z
dc.date.issued	2023-01-01
dc.identifier.citation	Forensic Genetic Approaches for Identification of Human Skeletal Remains: Challenges, Best Practices, and Emerging Technologies, 2023, pp. 137-163
dc.identifier.isbn	978-0-12-815766-4
dc.identifier.uri	http://hdl.handle.net/10453/173530
dc.description.abstract	This chapter introduces DNA quantification and the importance of assessing the quantity and quality of DNA recovered from human skeletal remains to ensure optimal performance of downstream assays. Quantification methods and instruments commonly used by forensic laboratories are presented, with a focus on real-time quantitative PCR (qPCR) techniques. The suitability of each qPCR method for determining the amount of amplifiable total human, male, and/or mitochondrial DNA, and for detecting the presence of co-extracted inhibitors or degraded DNA, is discussed. Finally, explanations on how this quantitative and qualitative sample information should be utilized to guide selection of genetic markers, DNA input amount, and procedural modifications for addressing sample degradation and inhibition are provided.
dc.format.extent	24
dc.language	en
dc.publisher	Elsevier
dc.relation.ispartof	Forensic Genetic Approaches for Identification of Human Skeletal Remains: Challenges, Best Practices, and Emerging Technologies
dc.relation.isbasedon	10.1016/B978-0-12-815766-4.00007-8
dc.rights	info:eu-repo/semantics/closedAccess
dc.title	Quantitative and qualitative assessment of DNA recovered from human skeletal remains
dc.type	Chapter
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2023-11-23T23:51:23Z
pubs.place-of-publication	UK
pubs.publication-status	Published
dc.location	UK

Abstract:

This chapter introduces DNA quantification and the importance of assessing the quantity and quality of DNA recovered from human skeletal remains to ensure optimal performance of downstream assays. Quantification methods and instruments commonly used by forensic laboratories are presented, with a focus on real-time quantitative PCR (qPCR) techniques. The suitability of each qPCR method for determining the amount of amplifiable total human, male, and/or mitochondrial DNA, and for detecting the presence of co-extracted inhibitors or degraded DNA, is discussed. Finally, explanations on how this quantitative and qualitative sample information should be utilized to guide selection of genetic markers, DNA input amount, and procedural modifications for addressing sample degradation and inhibition are provided.

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http://hdl.handle.net/10453/173530