Genotyping and sequencing of DNA recovered from human skeletal remains using capillary electrophoresis (CE)

Ward, J; Watherston, J; Kahline, I; McMahon, TP; Edson, SM

Genotyping and sequencing of DNA recovered from human skeletal remains using capillary electrophoresis (CE)

Ward, J

Watherston, J Kahline, I McMahon, TP Edson, SM

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Publisher:: Elsevier
Publication Type:: Chapter
Citation:: Forensic Genetic Approaches for Identification of Human Skeletal Remains: Challenges, Best Practices, and Emerging Technologies, 2023, pp. 285-323
Issue Date:: 2023-01-01

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Field	Value	Language
dc.contributor.author	Ward, J https://orcid.org/0000-0001-9359-9806
dc.contributor.author	Watherston, J
dc.contributor.author	Kahline, I
dc.contributor.author	McMahon, TP
dc.contributor.author	Edson, SM
dc.date.accessioned	2023-11-23T23:57:14Z
dc.date.available	2023-11-23T23:57:14Z
dc.date.issued	2023-01-01
dc.identifier.citation	Forensic Genetic Approaches for Identification of Human Skeletal Remains: Challenges, Best Practices, and Emerging Technologies, 2023, pp. 285-323
dc.identifier.isbn	978-0-12-815766-4
dc.identifier.uri	http://hdl.handle.net/10453/173531
dc.description.abstract	This chapter introduces capillary electrophoresis (CE) and how it is used for profiling DNA recovered from human skeletal remains. It will explain the most common fragment analysis and sequencing applications employed in forensic genetics, including the genotyping of autosomal short tandem repeats (STRs) and Sanger sequencing of mitochondrial DNA (mtDNA). The process of how PCR products are prepared, injected, separated, detected, and interpreted is discussed for each application. The CE platforms, chemistries, and analysis software commonly used by forensic laboratories are presented. Sample-specific CE artifacts often associated with skeletal samples are also detailed. Finally, the advantages and disadvantages of CE will be highlighted given the growing popularity of massively parallel sequencing (MPS).
dc.format.extent	24
dc.language	en
dc.publisher	Elsevier
dc.relation.ispartof	Forensic Genetic Approaches for Identification of Human Skeletal Remains: Challenges, Best Practices, and Emerging Technologies
dc.relation.isbasedon	10.1016/B978-0-12-815766-4.00014-5
dc.rights	info:eu-repo/semantics/closedAccess
dc.title	Genotyping and sequencing of DNA recovered from human skeletal remains using capillary electrophoresis (CE)
dc.type	Chapter
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2023-11-23T23:57:13Z
pubs.place-of-publication	UK
pubs.publication-status	Published
dc.location	UK

Abstract:

This chapter introduces capillary electrophoresis (CE) and how it is used for profiling DNA recovered from human skeletal remains. It will explain the most common fragment analysis and sequencing applications employed in forensic genetics, including the genotyping of autosomal short tandem repeats (STRs) and Sanger sequencing of mitochondrial DNA (mtDNA). The process of how PCR products are prepared, injected, separated, detected, and interpreted is discussed for each application. The CE platforms, chemistries, and analysis software commonly used by forensic laboratories are presented. Sample-specific CE artifacts often associated with skeletal samples are also detailed. Finally, the advantages and disadvantages of CE will be highlighted given the growing popularity of massively parallel sequencing (MPS).

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http://hdl.handle.net/10453/173531