Identification of Phytochemicals in Bioactive Extracts of Acacia saligna Growing in Australia.

Asmara, AP; Prasansuklab, A; Tencomnao, T; Ung, AT

Identification of Phytochemicals in Bioactive Extracts of Acacia saligna Growing in Australia.

Asmara, AP Prasansuklab, A Tencomnao, T Ung, AT

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Molecules, 2023, 28, (3), pp. 1028
Issue Date:: 2023-01-19

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Field	Value	Language
dc.contributor.author	Asmara, AP
dc.contributor.author	Prasansuklab, A
dc.contributor.author	Tencomnao, T
dc.contributor.author	Ung, AT
dc.date.accessioned	2023-12-05T04:16:17Z
dc.date.available	2023-01-16
dc.date.available	2023-12-05T04:16:17Z
dc.date.issued	2023-01-19
dc.identifier.citation	Molecules, 2023, 28, (3), pp. 1028
dc.identifier.issn	1420-3049
dc.identifier.issn	1420-3049
dc.identifier.uri	http://hdl.handle.net/10453/173691
dc.description.abstract	Acacia saligna growing in Australia has not been fully investigated for its bioactive phytochemicals. Sequential polarity-based extraction was employed to provide four different extracts from individual parts of A. saligna. Bioactive extracts were determined using in vitro antioxidant and yeast α-glucosidase inhibitory assays. Methanolic extracts from barks, leaves, and flowers are the most active and have no toxicity against 3T3-L1 adipocytes. Compound isolation of bioactive extracts provided us with ten compounds. Among them are two novel natural products; naringenin-7-O-α-L-arabinopyranoside 2 and (3S,5S)-3-hydroxy-5-(2-aminoethyl) dihydrofuran-2(3H)-one 9. D-(+)-pinitol 5a (from barks and flowers), (-)-pinitol 5b (exclusively from leaf), and 2,4-di-t-butylphenol 7 are known natural products and new to A. saligna. (-)-Epicatechin 6, quercitrin 4, and myricitrin 8 showed potent antioxidant activities consistently in DPPH and ABTS assays. (-)-Epicatechin 6 (IC50 = 63.58 μM),D-(+)-pinitol 5a (IC50 = 74.69 μM), and naringenin 1 (IC50 = 89.71 μM) are the strong inhibitors against the α-glucosidase enzyme. The presence of these compounds supports the activities exerted in our methanolic extracts. The presence of 2,4-di-t-butylphenol 7 may support the reported allelopathic and antifungal activities. The outcome of this study indicates the potential of Australian A. saligna as a rich source of bioactive compounds for drug discovery targeting type 2 diabetes.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI
dc.relation.ispartof	Molecules
dc.relation.isbasedon	10.3390/molecules28031028
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0304 Medicinal and Biomolecular Chemistry, 0305 Organic Chemistry, 0307 Theoretical and Computational Chemistry
dc.subject.classification	Organic Chemistry
dc.subject.classification	3404 Medicinal and biomolecular chemistry
dc.subject.classification	3405 Organic chemistry
dc.subject.mesh	Humans
dc.subject.mesh	Plant Extracts
dc.subject.mesh	Antioxidants
dc.subject.mesh	Acacia
dc.subject.mesh	Diabetes Mellitus, Type 2
dc.subject.mesh	Catechin
dc.subject.mesh	alpha-Glucosidases
dc.subject.mesh	Australia
dc.subject.mesh	Phytochemicals
dc.subject.mesh	Humans
dc.subject.mesh	Acacia
dc.subject.mesh	Diabetes Mellitus, Type 2
dc.subject.mesh	Catechin
dc.subject.mesh	alpha-Glucosidases
dc.subject.mesh	Plant Extracts
dc.subject.mesh	Antioxidants
dc.subject.mesh	Australia
dc.subject.mesh	Phytochemicals
dc.subject.mesh	Humans
dc.subject.mesh	Plant Extracts
dc.subject.mesh	Antioxidants
dc.subject.mesh	Acacia
dc.subject.mesh	Diabetes Mellitus, Type 2
dc.subject.mesh	Catechin
dc.subject.mesh	alpha-Glucosidases
dc.subject.mesh	Australia
dc.subject.mesh	Phytochemicals
dc.title	Identification of Phytochemicals in Bioactive Extracts of Acacia saligna Growing in Australia.
dc.type	Journal Article
utslib.citation.volume	28
utslib.location.activity	Switzerland
utslib.for	0304 Medicinal and Biomolecular Chemistry
utslib.for	0305 Organic Chemistry
utslib.for	0307 Theoretical and Computational Chemistry
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2023-12-05T04:16:03Z
pubs.issue	3
pubs.publication-status	Published online
pubs.volume	28
utslib.citation.issue	3

Abstract:

Acacia saligna growing in Australia has not been fully investigated for its bioactive phytochemicals. Sequential polarity-based extraction was employed to provide four different extracts from individual parts of A. saligna. Bioactive extracts were determined using in vitro antioxidant and yeast α-glucosidase inhibitory assays. Methanolic extracts from barks, leaves, and flowers are the most active and have no toxicity against 3T3-L1 adipocytes. Compound isolation of bioactive extracts provided us with ten compounds. Among them are two novel natural products; naringenin-7-O-α-L-arabinopyranoside 2 and (3S*,5S*)-3-hydroxy-5-(2-aminoethyl) dihydrofuran-2(3H)-one 9. D-(+)-pinitol 5a (from barks and flowers), (-)-pinitol 5b (exclusively from leaf), and 2,4-di-t-butylphenol 7 are known natural products and new to A. saligna. (-)-Epicatechin 6, quercitrin 4, and myricitrin 8 showed potent antioxidant activities consistently in DPPH and ABTS assays. (-)-Epicatechin 6 (IC50 = 63.58 μM),D-(+)-pinitol 5a (IC50 = 74.69 μM), and naringenin 1 (IC50 = 89.71 μM) are the strong inhibitors against the α-glucosidase enzyme. The presence of these compounds supports the activities exerted in our methanolic extracts. The presence of 2,4-di-t-butylphenol 7 may support the reported allelopathic and antifungal activities. The outcome of this study indicates the potential of Australian A. saligna as a rich source of bioactive compounds for drug discovery targeting type 2 diabetes.

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http://hdl.handle.net/10453/173691