Lipid stores reveal the state of the coral-algae symbiosis at the single-cell level.

Nielsen, DA; Petrou, K

Lipid stores reveal the state of the coral-algae symbiosis at the single-cell level.

Nielsen, DA Petrou, K

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Publisher:: SPRINGERNATURE
Publication Type:: Journal Article
Citation:: ISME Commun, 2023, 3, (1), pp. 29
Issue Date:: 2023-04-04

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Field	Value	Language
dc.contributor.author	Nielsen, DA
dc.contributor.author	Petrou, K https://orcid.org/0000-0002-2703-0694
dc.date.accessioned	2023-12-05T22:48:20Z
dc.date.available	2023-03-22
dc.date.available	2023-12-05T22:48:20Z
dc.date.issued	2023-04-04
dc.identifier.citation	ISME Commun, 2023, 3, (1), pp. 29
dc.identifier.issn	2730-6151
dc.identifier.issn	2730-6151
dc.identifier.uri	http://hdl.handle.net/10453/173743
dc.description.abstract	Coral reefs worldwide are threatened by environmental stress. The observable decline in coral cover, is principally due to the intensifying breakdown of the coral symbiosis, a process known as 'bleaching'. Overproduction of reactive oxygen species (ROS) is considered a key driver of coral bleaching, where environmental stress leads to increased ROS expression. To explore the link between ROS damage and symbiont status, we measured lipid peroxidation (LPO), a ubiquitous form of ROS damage, in the lipid stores of individual endo- and ex-symbiotic algal cells of three coral species, using confocal microscopy and a lipid hydroperoxide sensitive fluorescent dye. We found LPO was higher in endosymbionts, while lipid volume was greater in ex-symbiotic cells. Cluster analysis revealed three metabolic profiles differentiating endosymbiotic (#1: high LPO, low lipid) and ex-symbiotic cells (#3: low LPO, high lipid), with the intermediate group (#2) containing both cell types. Heat stress caused endosymbionts of Pocillopora acuta to shift away from cluster #1, suggesting this cluster represents cells in healthy/stable symbiosis. Our study delivers a new means to assess the coral symbiosis, demonstrating that symbiont LPO ratio combined with lipid store volume is a robust metabolic marker for the state of the symbiosis at the cellular level.
dc.format	Electronic
dc.language	eng
dc.publisher	SPRINGERNATURE
dc.relation.ispartof	ISME Commun
dc.relation.isbasedon	10.1038/s43705-023-00234-8
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	3103 Ecology
dc.subject.classification	3107 Microbiology
dc.title	Lipid stores reveal the state of the coral-algae symbiosis at the single-cell level.
dc.type	Journal Article
utslib.citation.volume	3
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2023-12-05T22:48:16Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	3
utslib.citation.issue	1

Abstract:

Coral reefs worldwide are threatened by environmental stress. The observable decline in coral cover, is principally due to the intensifying breakdown of the coral symbiosis, a process known as 'bleaching'. Overproduction of reactive oxygen species (ROS) is considered a key driver of coral bleaching, where environmental stress leads to increased ROS expression. To explore the link between ROS damage and symbiont status, we measured lipid peroxidation (LPO), a ubiquitous form of ROS damage, in the lipid stores of individual endo- and ex-symbiotic algal cells of three coral species, using confocal microscopy and a lipid hydroperoxide sensitive fluorescent dye. We found LPO was higher in endosymbionts, while lipid volume was greater in ex-symbiotic cells. Cluster analysis revealed three metabolic profiles differentiating endosymbiotic (#1: high LPO, low lipid) and ex-symbiotic cells (#3: low LPO, high lipid), with the intermediate group (#2) containing both cell types. Heat stress caused endosymbionts of Pocillopora acuta to shift away from cluster #1, suggesting this cluster represents cells in healthy/stable symbiosis. Our study delivers a new means to assess the coral symbiosis, demonstrating that symbiont LPO ratio combined with lipid store volume is a robust metabolic marker for the state of the symbiosis at the cellular level.

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http://hdl.handle.net/10453/173743