Early Alterations of PACAP and VIP Expression in the Female Rat Brain Following Spinal Cord Injury.

Broome, ST; Mandwie, M; Gorrie, CA; Musumeci, G; Marzagalli, R; Castorina, A

Early Alterations of PACAP and VIP Expression in the Female Rat Brain Following Spinal Cord Injury.

Broome, ST Mandwie, M Gorrie, CA Musumeci, G Marzagalli, R Castorina, A

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Publisher:: Springer Nature
Publication Type:: Journal Article
Citation:: J Mol Neurosci, 2023, 73, (9-10), pp. 724-737
Issue Date:: 2023-10

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Field	Value	Language
dc.contributor.author	Broome, ST
dc.contributor.author	Mandwie, M
dc.contributor.author	Gorrie, CA
dc.contributor.author	Musumeci, G
dc.contributor.author	Marzagalli, R
dc.contributor.author	Castorina, A https://orcid.org/0000-0001-7037-759X
dc.date.accessioned	2024-01-08T05:21:35Z
dc.date.available	2023-08-21
dc.date.available	2024-01-08T05:21:35Z
dc.date.issued	2023-10
dc.identifier.citation	J Mol Neurosci, 2023, 73, (9-10), pp. 724-737
dc.identifier.issn	0895-8696
dc.identifier.issn	1559-1166
dc.identifier.uri	http://hdl.handle.net/10453/174097
dc.description.abstract	Previous evidence shows that rapid changes occur in the brain following spinal cord injury (SCI). Here, we interrogated the expression of the neuropeptides pituitary adenylyl cyclase-activating peptide (PACAP), vasoactive intestinal peptides (VIP), and their binding receptors in the rat brain 24 h following SCI. Female Sprague-Dawley rats underwent thoracic laminectomy; half of the rats received a mild contusion injury at the level of the T10 vertebrate (SCI group); the other half underwent sham surgery (sham group). Twenty-four hours post-surgery, the hypothalamus, thalamus, amygdala, hippocampus (dorsal and ventral), prefrontal cortex, and periaqueductal gray were collected. PACAP, VIP, PAC1, VPAC1, and VPAC2 mRNA and protein levels were measured by real-time quantitative polymerase chain reaction and Western blot. In SCI rats, PACAP expression was increased in the hypothalamus (104-141% vs sham) and amygdala (138-350%), but downregulated in the thalamus (35-95%) and periaqueductal gray (58-68%). VIP expression was increased only in the thalamus (175-385%), with a reduction in the amygdala (51-68%), hippocampus (40-75%), and periaqueductal gray (74-76%). The expression of the PAC1 receptor was the least disturbed by SCI, with decrease expression in the ventral hippocampus (63-68%) only. The expression levels of VPAC1 and VPAC2 receptors were globally reduced, with more prominent reductions of VPAC1 vs VPAC2 in the amygdala (21-70%) and ventral hippocampus (72-75%). In addition, VPAC1 downregulation also extended to the dorsal hippocampus (69-70%). These findings demonstrate that as early as 24 h post-SCI, there are region-specific disruptions of PACAP, VIP, and related receptor transcript and protein levels in supraspinal regions controlling higher cognitive functions.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Springer Nature
dc.relation.ispartof	J Mol Neurosci
dc.relation.isbasedon	10.1007/s12031-023-02151-w
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1109 Neurosciences, 1702 Cognitive Sciences
dc.subject.classification	Neurology & Neurosurgery
dc.subject.classification	3101 Biochemistry and cell biology
dc.subject.classification	3209 Neurosciences
dc.subject.mesh	Female
dc.subject.mesh	Rats
dc.subject.mesh	Animals
dc.subject.mesh	Pituitary Adenylate Cyclase-Activating Polypeptide
dc.subject.mesh	Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
dc.subject.mesh	Rats, Sprague-Dawley
dc.subject.mesh	Receptors, Pituitary Hormone
dc.subject.mesh	Vasoactive Intestinal Peptide
dc.subject.mesh	Receptors, Vasoactive Intestinal Polypeptide, Type I
dc.subject.mesh	Receptors, Vasoactive Intestinal Peptide, Type II
dc.subject.mesh	Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
dc.subject.mesh	Spinal Cord Injuries
dc.subject.mesh	Brain
dc.subject.mesh	Brain
dc.subject.mesh	Animals
dc.subject.mesh	Rats
dc.subject.mesh	Rats, Sprague-Dawley
dc.subject.mesh	Spinal Cord Injuries
dc.subject.mesh	Vasoactive Intestinal Peptide
dc.subject.mesh	Receptors, Pituitary Hormone
dc.subject.mesh	Female
dc.subject.mesh	Pituitary Adenylate Cyclase-Activating Polypeptide
dc.subject.mesh	Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
dc.subject.mesh	Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
dc.subject.mesh	Receptors, Vasoactive Intestinal Polypeptide, Type I
dc.subject.mesh	Receptors, Vasoactive Intestinal Peptide, Type II
dc.title	Early Alterations of PACAP and VIP Expression in the Female Rat Brain Following Spinal Cord Injury.
dc.type	Journal Article
utslib.citation.volume	73
utslib.location.activity	United States
utslib.for	1109 Neurosciences
utslib.for	1702 Cognitive Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2024-01-08T05:21:26Z
pubs.issue	9-10
pubs.publication-status	Published
pubs.volume	73
utslib.citation.issue	9-10

Abstract:

Previous evidence shows that rapid changes occur in the brain following spinal cord injury (SCI). Here, we interrogated the expression of the neuropeptides pituitary adenylyl cyclase-activating peptide (PACAP), vasoactive intestinal peptides (VIP), and their binding receptors in the rat brain 24 h following SCI. Female Sprague-Dawley rats underwent thoracic laminectomy; half of the rats received a mild contusion injury at the level of the T10 vertebrate (SCI group); the other half underwent sham surgery (sham group). Twenty-four hours post-surgery, the hypothalamus, thalamus, amygdala, hippocampus (dorsal and ventral), prefrontal cortex, and periaqueductal gray were collected. PACAP, VIP, PAC1, VPAC1, and VPAC2 mRNA and protein levels were measured by real-time quantitative polymerase chain reaction and Western blot. In SCI rats, PACAP expression was increased in the hypothalamus (104-141% vs sham) and amygdala (138-350%), but downregulated in the thalamus (35-95%) and periaqueductal gray (58-68%). VIP expression was increased only in the thalamus (175-385%), with a reduction in the amygdala (51-68%), hippocampus (40-75%), and periaqueductal gray (74-76%). The expression of the PAC1 receptor was the least disturbed by SCI, with decrease expression in the ventral hippocampus (63-68%) only. The expression levels of VPAC1 and VPAC2 receptors were globally reduced, with more prominent reductions of VPAC1 vs VPAC2 in the amygdala (21-70%) and ventral hippocampus (72-75%). In addition, VPAC1 downregulation also extended to the dorsal hippocampus (69-70%). These findings demonstrate that as early as 24 h post-SCI, there are region-specific disruptions of PACAP, VIP, and related receptor transcript and protein levels in supraspinal regions controlling higher cognitive functions.

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http://hdl.handle.net/10453/174097