The mechanism of Pseudomonas aeruginosa outer membrane vesicle biogenesis determines their protein composition.

Zavan, L; Fang, H; Johnston, EL; Whitchurch, C; Greening, DW; Hill, AF; Kaparakis-Liaskos, M

The mechanism of Pseudomonas aeruginosa outer membrane vesicle biogenesis determines their protein composition.

Zavan, L Fang, H Johnston, EL Whitchurch, C

Greening, DW Hill, AF Kaparakis-Liaskos, M

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Publisher:: Wiley
Publication Type:: Journal Article
Citation:: Proteomics, 2023, 23, (10), pp. e2200464
Issue Date:: 2023-05

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Field	Value	Language
dc.contributor.author	Zavan, L
dc.contributor.author	Fang, H
dc.contributor.author	Johnston, EL
dc.contributor.author	Whitchurch, C https://orcid.org/0000-0003-2296-3791
dc.contributor.author	Greening, DW
dc.contributor.author	Hill, AF
dc.contributor.author	Kaparakis-Liaskos, M
dc.date.accessioned	2024-01-10T23:06:09Z
dc.date.available	2023-02-03
dc.date.available	2024-01-10T23:06:09Z
dc.date.issued	2023-05
dc.identifier.citation	Proteomics, 2023, 23, (10), pp. e2200464
dc.identifier.issn	1615-9853
dc.identifier.issn	1615-9861
dc.identifier.uri	http://hdl.handle.net/10453/174241
dc.description.abstract	Gram-negative bacteria produce outer membrane vesicles (OMVs) and contain bacterial cargo including nucleic acids and proteins. The proteome of OMVs can be altered by various factors including bacterial growth stage, growth conditions, and environmental factors. However, it is currently unknown if the mechanism of OMV biogenesis can determine their proteome. In this study, we examined whether the mechanisms of OMV biogenesis influenced the production and protein composition of Pseudomonas aeruginosa OMVs. OMVs were isolated from three P. aeruginosa strains that produced OMVs either by budding alone, by explosive cell lysis, or by both budding and explosive cell lysis. We identified that the mechanism of OMV biogenesis dictated OMV quantity. Furthermore, a global proteomic analysis comparing the proteome of OMVs to their parent bacteria showed significant differences in the identification of proteins in bacteria and OMVs. Finally, we determined that the mechanism of OMV biogenesis influenced the protein composition of OMVs, as OMVs released by distinct mechanisms of biogenesis differed significantly from one another in their proteome and functional enrichment analysis. Overall, our findings reveal that the mechanism of OMV biogenesis is a main factor that determines the OMV proteome which may affect their subsequent biological functions.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Wiley
dc.relation.ispartof	Proteomics
dc.relation.isbasedon	10.1002/pmic.202200464
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	06 Biological Sciences, 08 Information and Computing Sciences, 11 Medical and Health Sciences
dc.subject.classification	Biochemistry & Molecular Biology
dc.subject.classification	31 Biological sciences
dc.subject.classification	32 Biomedical and clinical sciences
dc.subject.mesh	Pseudomonas aeruginosa
dc.subject.mesh	Proteome
dc.subject.mesh	Proteomics
dc.subject.mesh	Exosomes
dc.subject.mesh	Gram-Negative Bacteria
dc.subject.mesh	Bacterial Outer Membrane Proteins
dc.subject.mesh	Gram-Negative Bacteria
dc.subject.mesh	Pseudomonas aeruginosa
dc.subject.mesh	Bacterial Outer Membrane Proteins
dc.subject.mesh	Proteome
dc.subject.mesh	Proteomics
dc.subject.mesh	Exosomes
dc.subject.mesh	Pseudomonas aeruginosa
dc.subject.mesh	Proteome
dc.subject.mesh	Proteomics
dc.subject.mesh	Exosomes
dc.subject.mesh	Gram-Negative Bacteria
dc.subject.mesh	Bacterial Outer Membrane Proteins
dc.title	The mechanism of Pseudomonas aeruginosa outer membrane vesicle biogenesis determines their protein composition.
dc.type	Journal Article
utslib.citation.volume	23
utslib.location.activity	Germany
utslib.for	06 Biological Sciences
utslib.for	08 Information and Computing Sciences
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.date.updated	2024-01-10T23:06:07Z
pubs.issue	10
pubs.publication-status	Published
pubs.volume	23
utslib.citation.issue	10

Abstract:

Gram-negative bacteria produce outer membrane vesicles (OMVs) and contain bacterial cargo including nucleic acids and proteins. The proteome of OMVs can be altered by various factors including bacterial growth stage, growth conditions, and environmental factors. However, it is currently unknown if the mechanism of OMV biogenesis can determine their proteome. In this study, we examined whether the mechanisms of OMV biogenesis influenced the production and protein composition of Pseudomonas aeruginosa OMVs. OMVs were isolated from three P. aeruginosa strains that produced OMVs either by budding alone, by explosive cell lysis, or by both budding and explosive cell lysis. We identified that the mechanism of OMV biogenesis dictated OMV quantity. Furthermore, a global proteomic analysis comparing the proteome of OMVs to their parent bacteria showed significant differences in the identification of proteins in bacteria and OMVs. Finally, we determined that the mechanism of OMV biogenesis influenced the protein composition of OMVs, as OMVs released by distinct mechanisms of biogenesis differed significantly from one another in their proteome and functional enrichment analysis. Overall, our findings reveal that the mechanism of OMV biogenesis is a main factor that determines the OMV proteome which may affect their subsequent biological functions.

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http://hdl.handle.net/10453/174241