Increased iron utilization and oxidative stress tolerance in a Vibrio cholerae flrA mutant confers resistance to amoeba predation.

Hoque, MM; Noorian, P; Espinoza-Vergara, G; Adhikary, S; To, J; Rice, SA; McDougald, D

Increased iron utilization and oxidative stress tolerance in a Vibrio cholerae flrA mutant confers resistance to amoeba predation.

Hoque, MM Noorian, P

Espinoza-Vergara, G Adhikary, S To, J

Rice, SA McDougald, D

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Publisher:: AMER SOC MICROBIOLOGY
Publication Type:: Journal Article
Citation:: Appl Environ Microbiol, 2023, 89, (11), pp. e0109523
Issue Date:: 2023-11-29

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Field	Value	Language
dc.contributor.author	Hoque, MM
dc.contributor.author	Noorian, P https://orcid.org/0000-0002-4215-1080
dc.contributor.author	Espinoza-Vergara, G
dc.contributor.author	Adhikary, S
dc.contributor.author	To, J https://orcid.org/0000-0003-3482-4369
dc.contributor.author	Rice, SA
dc.contributor.author	McDougald, D
dc.contributor.editor	Alexandre, G
dc.date.accessioned	2024-01-10T23:33:12Z
dc.date.available	2024-01-10T23:33:12Z
dc.date.issued	2023-11-29
dc.identifier.citation	Appl Environ Microbiol, 2023, 89, (11), pp. e0109523
dc.identifier.issn	0099-2240
dc.identifier.issn	1098-5336
dc.identifier.uri	http://hdl.handle.net/10453/174247
dc.description.abstract	Persistence of V. cholerae in the aquatic environment contributes to the fatal diarrheal disease cholera, which remains a global health burden. In the environment, bacteria face predation pressure by heterotrophic protists such as the free-living amoeba A. castellanii. This study explores how a mutant of V. cholerae adapts to acquire essential nutrients and survive predation. Here, we observed that up-regulation of iron acquisition genes and genes regulating resistance to oxidative stress enhances pathogen fitness. Our data show that V. cholerae can defend predation to overcome nutrient limitation and oxidative stress, resulting in an enhanced survival inside the protozoan hosts.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER SOC MICROBIOLOGY
dc.relation	http://purl.org/au-research/grants/arc/DP170100453
dc.relation.ispartof	Appl Environ Microbiol
dc.relation.isbasedon	10.1128/aem.01095-23
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Microbiology
dc.subject.classification	3107 Microbiology
dc.subject.classification	3207 Medical microbiology
dc.subject.mesh	Animals
dc.subject.mesh	Vibrio cholerae
dc.subject.mesh	Amoeba
dc.subject.mesh	Predatory Behavior
dc.subject.mesh	Cholera
dc.subject.mesh	Iron
dc.subject.mesh	Animals
dc.subject.mesh	Amoeba
dc.subject.mesh	Vibrio cholerae
dc.subject.mesh	Cholera
dc.subject.mesh	Iron
dc.subject.mesh	Predatory Behavior
dc.title	Increased iron utilization and oxidative stress tolerance in a Vibrio cholerae flrA mutant confers resistance to amoeba predation.
dc.type	Journal Article
utslib.citation.volume	89
utslib.location.activity	United States
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - AIMI - Australian Institute for Microbiology & Infection
utslib.copyright.status	closed_access	*
dc.date.updated	2024-01-10T23:33:07Z
pubs.issue	11
pubs.publication-status	Published
pubs.volume	89
utslib.citation.issue	11

Abstract:

Persistence of V. cholerae in the aquatic environment contributes to the fatal diarrheal disease cholera, which remains a global health burden. In the environment, bacteria face predation pressure by heterotrophic protists such as the free-living amoeba A. castellanii. This study explores how a mutant of V. cholerae adapts to acquire essential nutrients and survive predation. Here, we observed that up-regulation of iron acquisition genes and genes regulating resistance to oxidative stress enhances pathogen fitness. Our data show that V. cholerae can defend predation to overcome nutrient limitation and oxidative stress, resulting in an enhanced survival inside the protozoan hosts.

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http://hdl.handle.net/10453/174247