Systematic analyses identify modes of action of ten clinically relevant biocides and antibiotic antagonism in Acinetobacter baumannii.

Li, L; Short, FL; Hassan, KA; Naidu, V; Pokhrel, A; Nagy, SS; Prity, FT; Shah, BS; Afrin, N; Baker, S; Parkhill, J; Cain, AK; Paulsen, IT

Systematic analyses identify modes of action of ten clinically relevant biocides and antibiotic antagonism in Acinetobacter baumannii.

Li, L Short, FL Hassan, KA Naidu, V Pokhrel, A

Nagy, SS Prity, FT Shah, BS Afrin, N Baker, S Parkhill, J Cain, AK Paulsen, IT

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Publisher:: NATURE PORTFOLIO
Publication Type:: Journal Article
Citation:: Nat Microbiol, 2023, 8, (11), pp. 1995-2005
Issue Date:: 2023-11

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Field	Value	Language
dc.contributor.author	Li, L
dc.contributor.author	Short, FL
dc.contributor.author	Hassan, KA
dc.contributor.author	Naidu, V
dc.contributor.author	Pokhrel, A https://orcid.org/0000-0001-8897-2397
dc.contributor.author	Nagy, SS
dc.contributor.author	Prity, FT
dc.contributor.author	Shah, BS
dc.contributor.author	Afrin, N
dc.contributor.author	Baker, S
dc.contributor.author	Parkhill, J
dc.contributor.author	Cain, AK
dc.contributor.author	Paulsen, IT
dc.date.accessioned	2024-01-11T03:57:08Z
dc.date.available	2023-08-11
dc.date.available	2024-01-11T03:57:08Z
dc.date.issued	2023-11
dc.identifier.citation	Nat Microbiol, 2023, 8, (11), pp. 1995-2005
dc.identifier.issn	2058-5276
dc.identifier.issn	2058-5276
dc.identifier.uri	http://hdl.handle.net/10453/174302
dc.description.abstract	Concerns exist that widespread use of antiseptic or disinfectant biocides could contribute to the emergence and spread of multidrug-resistant bacteria. To investigate this, we performed transposon-directed insertion-site sequencing (TraDIS) on the multidrug-resistant pathogen, Acinetobacter baumannii, exposed to a panel of ten structurally diverse and clinically relevant biocides. Multiple gene targets encoding cell envelope or cytoplasmic proteins involved in processes including fatty acid biogenesis, multidrug efflux, the tricarboxylic acid cycle, cell respiration and cell division, were identified to have effects on bacterial fitness upon biocide exposure, suggesting that these compounds may have intracellular targets in addition to their known effects on the cell envelope. As cell respiration genes are required for A. baumannii fitness in biocides, we confirmed that sub-inhibitory concentrations of the biocides that dissipate membrane potential can promote A. baumannii tolerance to antibiotics that act intracellularly. Our results support the concern that residual biocides might promote antibiotic resistance in pathogenic bacteria.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	NATURE PORTFOLIO
dc.relation.ispartof	Nat Microbiol
dc.relation.isbasedon	10.1038/s41564-023-01474-z
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0605 Microbiology, 1108 Medical Microbiology
dc.subject.classification	3107 Microbiology
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Disinfectants
dc.subject.mesh	Acinetobacter baumannii
dc.subject.mesh	Drug Resistance, Bacterial
dc.subject.mesh	Bacteria
dc.subject.mesh	Bacteria
dc.subject.mesh	Acinetobacter baumannii
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Disinfectants
dc.subject.mesh	Drug Resistance, Bacterial
dc.title	Systematic analyses identify modes of action of ten clinically relevant biocides and antibiotic antagonism in Acinetobacter baumannii.
dc.type	Journal Article
utslib.citation.volume	8
utslib.location.activity	England
utslib.for	0605 Microbiology
utslib.for	1108 Medical Microbiology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access	*
dc.date.updated	2024-01-11T03:57:06Z
pubs.issue	11
pubs.publication-status	Published
pubs.volume	8
utslib.citation.issue	11

Abstract:

Concerns exist that widespread use of antiseptic or disinfectant biocides could contribute to the emergence and spread of multidrug-resistant bacteria. To investigate this, we performed transposon-directed insertion-site sequencing (TraDIS) on the multidrug-resistant pathogen, Acinetobacter baumannii, exposed to a panel of ten structurally diverse and clinically relevant biocides. Multiple gene targets encoding cell envelope or cytoplasmic proteins involved in processes including fatty acid biogenesis, multidrug efflux, the tricarboxylic acid cycle, cell respiration and cell division, were identified to have effects on bacterial fitness upon biocide exposure, suggesting that these compounds may have intracellular targets in addition to their known effects on the cell envelope. As cell respiration genes are required for A. baumannii fitness in biocides, we confirmed that sub-inhibitory concentrations of the biocides that dissipate membrane potential can promote A. baumannii tolerance to antibiotics that act intracellularly. Our results support the concern that residual biocides might promote antibiotic resistance in pathogenic bacteria.

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http://hdl.handle.net/10453/174302