Toward Microbiome Engineering: Expanding the Repertoire of Genetically Tractable Members of the Human Gut Microbiome.

Marsh, JW; Kirk, C; Ley, RE

Toward Microbiome Engineering: Expanding the Repertoire of Genetically Tractable Members of the Human Gut Microbiome.

Marsh, JW Kirk, C Ley, RE

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Publisher:: ANNUAL REVIEWS
Publication Type:: Journal Article
Citation:: Annu Rev Microbiol, 2023, 77, (1), pp. 427-449
Issue Date:: 2023-09-15

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Field	Value	Language
dc.contributor.author	Marsh, JW
dc.contributor.author	Kirk, C
dc.contributor.author	Ley, RE
dc.date.accessioned	2024-01-11T04:16:35Z
dc.date.available	2024-01-11T04:16:35Z
dc.date.issued	2023-09-15
dc.identifier.citation	Annu Rev Microbiol, 2023, 77, (1), pp. 427-449
dc.identifier.issn	0066-4227
dc.identifier.issn	1545-3251
dc.identifier.uri	http://hdl.handle.net/10453/174305
dc.description.abstract	Genetic manipulation is necessary to interrogate the functions of microbes in their environments, such as the human gut microbiome. Yet, the vast majority of human gut microbiome species are not genetically tractable. Here, we review the hurdles to seizing genetic control of more species. We address the barriers preventing the application of genetic techniques to gut microbes and report on genetic systems currently under development. While methods aimed at genetically transforming many species simultaneously in situ show promise, they are unable to overcome many of the same challenges that exist for individual microbes. Unless a major conceptual breakthrough emerges, the genetic tractability of the microbiome will remain an arduous task. Increasing the list of genetically tractable organisms from the human gut remains one of the highest priorities for microbiome research and will provide the foundation for microbiome engineering.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ANNUAL REVIEWS
dc.relation.ispartof	Annu Rev Microbiol
dc.relation.isbasedon	10.1146/annurev-micro-032421-112304
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0605 Microbiology, 1108 Medical Microbiology
dc.subject.classification	Microbiology
dc.subject.classification	3107 Microbiology
dc.subject.mesh	Humans
dc.subject.mesh	Gastrointestinal Microbiome
dc.subject.mesh	Microbiota
dc.subject.mesh	Humans
dc.subject.mesh	Gastrointestinal Microbiome
dc.subject.mesh	Microbiota
dc.title	Toward Microbiome Engineering: Expanding the Repertoire of Genetically Tractable Members of the Human Gut Microbiome.
dc.type	Journal Article
utslib.citation.volume	77
utslib.location.activity	United States
utslib.for	0605 Microbiology
utslib.for	1108 Medical Microbiology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.date.updated	2024-01-11T04:16:33Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	77
utslib.citation.issue	1

Abstract:

Genetic manipulation is necessary to interrogate the functions of microbes in their environments, such as the human gut microbiome. Yet, the vast majority of human gut microbiome species are not genetically tractable. Here, we review the hurdles to seizing genetic control of more species. We address the barriers preventing the application of genetic techniques to gut microbes and report on genetic systems currently under development. While methods aimed at genetically transforming many species simultaneously in situ show promise, they are unable to overcome many of the same challenges that exist for individual microbes. Unless a major conceptual breakthrough emerges, the genetic tractability of the microbiome will remain an arduous task. Increasing the list of genetically tractable organisms from the human gut remains one of the highest priorities for microbiome research and will provide the foundation for microbiome engineering.

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http://hdl.handle.net/10453/174305