Single Microcolony Diffusion Analysis in Pseudomonas aeruginosa Biofilms.

Sankaran, J; Rice, SA; Wohland, T

Single Microcolony Diffusion Analysis in Pseudomonas aeruginosa Biofilms.

Sankaran, J Rice, SA Wohland, T

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Publisher:: Springer Nature
Publication Type:: Chapter
Citation:: Methods in Molecular Biology, 2024, 2721, pp. 153-168
Issue Date:: 2024

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Field	Value	Language
dc.contributor.author	Sankaran, J
dc.contributor.author	Rice, SA
dc.contributor.author	Wohland, T
dc.date.accessioned	2024-01-12T02:33:43Z
dc.date.available	2024-01-12T02:33:43Z
dc.date.issued	2024
dc.identifier.citation	Methods in Molecular Biology, 2024, 2721, pp. 153-168
dc.identifier.isbn	9781071634721
dc.identifier.uri	http://hdl.handle.net/10453/174341
dc.description.abstract	Diffusion phenomena play an important role during various stages of biofilm development. Hence, quantification of the diffusion coefficient of molecules provides important information necessary to understand the variability in diffusion profiles at different biofilm stages. Imaging fluorescence correlation spectroscopy (FCS) enables the visualization of 3D diffusion profiles of biofilms. In this protocol, we explain how to grow biofilms for investigation using imaging FCS and how to acquire and analyze the data.
dc.format	Print
dc.language	en
dc.publisher	Springer Nature
dc.relation.ispartof	Methods in Molecular Biology
dc.relation.ispartofseries	Methods in Molecular Biology
dc.relation.isbasedon	10.1007/978-1-0716-3473-8_11
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.subject.classification	3101 Biochemistry and cell biology
dc.subject.classification	3404 Medicinal and biomolecular chemistry
dc.subject.mesh	Pseudomonas aeruginosa
dc.subject.mesh	Biofilms
dc.subject.mesh	Biofilms
dc.subject.mesh	Pseudomonas aeruginosa
dc.subject.mesh	Pseudomonas aeruginosa
dc.subject.mesh	Biofilms
dc.title	Single Microcolony Diffusion Analysis in Pseudomonas aeruginosa Biofilms.
dc.type	Chapter
utslib.citation.volume	2721
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access	*
dc.date.updated	2024-01-12T02:33:42Z
pubs.publication-status	Published
pubs.volume	2721

Abstract:

Diffusion phenomena play an important role during various stages of biofilm development. Hence, quantification of the diffusion coefficient of molecules provides important information necessary to understand the variability in diffusion profiles at different biofilm stages. Imaging fluorescence correlation spectroscopy (FCS) enables the visualization of 3D diffusion profiles of biofilms. In this protocol, we explain how to grow biofilms for investigation using imaging FCS and how to acquire and analyze the data.

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http://hdl.handle.net/10453/174341