Population Structure and Genomic Characteristics of Australian Erysipelothrix rhusiopathiae Reveals Unobserved Diversity in the Australian Pig Industry.

Webster, J; Bowring, B; Stroud, L; Marsh, I; Sales, N; Bogema, D

Population Structure and Genomic Characteristics of Australian Erysipelothrix rhusiopathiae Reveals Unobserved Diversity in the Australian Pig Industry.

Webster, J Bowring, B Stroud, L Marsh, I Sales, N Bogema, D

Permalink

Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Microorganisms, 2023, 11, (2), pp. 297
Issue Date:: 2023-01-23

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published versionAdobe PDF (1.22 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Webster, J
dc.contributor.author	Bowring, B
dc.contributor.author	Stroud, L
dc.contributor.author	Marsh, I
dc.contributor.author	Sales, N
dc.contributor.author	Bogema, D https://orcid.org/0000-0001-7013-7326
dc.date.accessioned	2024-01-12T04:05:18Z
dc.date.available	2023-01-19
dc.date.available	2024-01-12T04:05:18Z
dc.date.issued	2023-01-23
dc.identifier.citation	Microorganisms, 2023, 11, (2), pp. 297
dc.identifier.issn	2076-2607
dc.identifier.issn	2076-2607
dc.identifier.uri	http://hdl.handle.net/10453/174365
dc.description.abstract	Erysipelothrix rhusiopathiae is a bacterial pathogen that is the causative agent of erysipelas in a variety of animals, including swine, emus, turkeys, muskox, caribou, moose, and humans. This study aims to investigate the population structure and genomic features of Australian isolates of E. rhusiopathiae in the Australian pig industry and compare them to the broader scope of isolates worldwide. A total of 178 isolates (154 Australian, seven vaccine isolates, six international isolates, and 11 of unknown origin) in this study were screened against an MLST scheme and publicly available reference isolates, identifying 59 new alleles, with isolates separating into two main single locus variant groups. Investigation with BLASTn revealed the presence of the spaA gene in 171 (96%) of the isolates, with three main groups of SpaA protein sequences observed amongst the isolates. Novel SpaA protein sequences, categorised here as group 3 sequences, consisted of two sequence types forming separate clades to groups 1 and 2, with amino acid variants at positions 195 (D/A), 303 (G/E) and 323(P/L). In addition to the newly identified groups, five new variant positions were identified, 124 (S/N), 307 (Q/R), 323 (P/L), 379 (M/I), and 400 (V/I). Resistance screening identified genes related to lincomycin, streptomycin, erythromycin, and tetracycline resistance. Of the 29 isolates carrying these resistance genes, 82% belonged to SpaA group 2-N101S (n = 22) or 2-N101S-I257L (n = 2). In addition, 79% (n = 23) of these 29 isolates belonged to MLST group ST 5. Our results illustrate that Australia appears to have a unique diversity of E. rhusiopathiae isolates in pig production industries within the wider global context of isolates.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI
dc.relation.ispartof	Microorganisms
dc.relation.isbasedon	10.3390/microorganisms11020297
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	3107 Microbiology
dc.subject.classification	3207 Medical microbiology
dc.title	Population Structure and Genomic Characteristics of Australian Erysipelothrix rhusiopathiae Reveals Unobserved Diversity in the Australian Pig Industry.
dc.type	Journal Article
utslib.citation.volume	11
utslib.location.activity	Switzerland
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.date.updated	2024-01-12T04:05:14Z
pubs.issue	2
pubs.publication-status	Published online
pubs.volume	11
utslib.citation.issue	2

Abstract:

Erysipelothrix rhusiopathiae is a bacterial pathogen that is the causative agent of erysipelas in a variety of animals, including swine, emus, turkeys, muskox, caribou, moose, and humans. This study aims to investigate the population structure and genomic features of Australian isolates of E. rhusiopathiae in the Australian pig industry and compare them to the broader scope of isolates worldwide. A total of 178 isolates (154 Australian, seven vaccine isolates, six international isolates, and 11 of unknown origin) in this study were screened against an MLST scheme and publicly available reference isolates, identifying 59 new alleles, with isolates separating into two main single locus variant groups. Investigation with BLASTn revealed the presence of the spaA gene in 171 (96%) of the isolates, with three main groups of SpaA protein sequences observed amongst the isolates. Novel SpaA protein sequences, categorised here as group 3 sequences, consisted of two sequence types forming separate clades to groups 1 and 2, with amino acid variants at positions 195 (D/A), 303 (G/E) and 323(P/L). In addition to the newly identified groups, five new variant positions were identified, 124 (S/N), 307 (Q/R), 323 (P/L), 379 (M/I), and 400 (V/I). Resistance screening identified genes related to lincomycin, streptomycin, erythromycin, and tetracycline resistance. Of the 29 isolates carrying these resistance genes, 82% belonged to SpaA group 2-N101S (n = 22) or 2-N101S-I257L (n = 2). In addition, 79% (n = 23) of these 29 isolates belonged to MLST group ST 5. Our results illustrate that Australia appears to have a unique diversity of E. rhusiopathiae isolates in pig production industries within the wider global context of isolates.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/174365