Thrombopoietin-independent generation of platelet-like particles from megakaryoblastic cells.

Nunthanasup, N; Ketprasit, N; Noulsri, E; Palasuwan, A; Combes, V; Kulkeaw, K; Palasuwan, D

Thrombopoietin-independent generation of platelet-like particles from megakaryoblastic cells.

Nunthanasup, N Ketprasit, N Noulsri, E Palasuwan, A Combes, V

Kulkeaw, K Palasuwan, D

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Publisher:: Springer Science and Business Media LLC
Publication Type:: Journal Article
Citation:: Sci Rep, 2023, 13, (1), pp. 22553
Issue Date:: 2023-12-18

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Field	Value	Language
dc.contributor.author	Nunthanasup, N
dc.contributor.author	Ketprasit, N
dc.contributor.author	Noulsri, E
dc.contributor.author	Palasuwan, A
dc.contributor.author	Combes, V https://orcid.org/0000-0003-2178-3596
dc.contributor.author	Kulkeaw, K
dc.contributor.author	Palasuwan, D
dc.date.accessioned	2024-01-16T00:22:52Z
dc.date.available	2023-12-15
dc.date.available	2024-01-16T00:22:52Z
dc.date.issued	2023-12-18
dc.identifier.citation	Sci Rep, 2023, 13, (1), pp. 22553
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/174511
dc.description.abstract	The use of megakaryoblastic leukemia MEG-01 cells can help reveal the mechanisms of thrombopoiesis. However, conventional in vitro activation of platelet release from MEG-01 cells requires thrombopoietin, which is costly. Here, we aim to develop a more straightforward and affordable method. Synchronization of the MEG-01 cells was initially performed using serum-free culture, followed by spontaneous cell differentiation in the presence of serum. Different stages of megakaryoblast differentiation were classified based on cell morphology, DNA content, and cell cycle. The MEG-01 cells released platelet-like particles at a level comparable to that of the thrombopoietin-activated MEG-01 cells. The platelet-like particles were distinguishable from PLP-derived extracellular vesicles and could express P-selectin following ADP activation. Importantly, the platelet-like particles induced fibrin clotting in vitro using platelet-poor plasma. Therefore, this thrombopoietin-independent cell synchronization method is an effective and straightforward method for studying megakaryopoiesis and thrombopoiesis.
dc.format	Electronic
dc.language	eng
dc.publisher	Springer Science and Business Media LLC
dc.relation.ispartof	Sci Rep
dc.relation.isbasedon	10.1038/s41598-023-50111-6
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Megakaryocytes
dc.subject.mesh	Thrombopoietin
dc.subject.mesh	Megakaryocyte Progenitor Cells
dc.subject.mesh	Blood Platelets
dc.subject.mesh	Thrombopoiesis
dc.title	Thrombopoietin-independent generation of platelet-like particles from megakaryoblastic cells.
dc.type	Journal Article
utslib.citation.volume	13
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2024-01-16T00:22:50Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	13
utslib.citation.issue	1

Abstract:

The use of megakaryoblastic leukemia MEG-01 cells can help reveal the mechanisms of thrombopoiesis. However, conventional in vitro activation of platelet release from MEG-01 cells requires thrombopoietin, which is costly. Here, we aim to develop a more straightforward and affordable method. Synchronization of the MEG-01 cells was initially performed using serum-free culture, followed by spontaneous cell differentiation in the presence of serum. Different stages of megakaryoblast differentiation were classified based on cell morphology, DNA content, and cell cycle. The MEG-01 cells released platelet-like particles at a level comparable to that of the thrombopoietin-activated MEG-01 cells. The platelet-like particles were distinguishable from PLP-derived extracellular vesicles and could express P-selectin following ADP activation. Importantly, the platelet-like particles induced fibrin clotting in vitro using platelet-poor plasma. Therefore, this thrombopoietin-independent cell synchronization method is an effective and straightforward method for studying megakaryopoiesis and thrombopoiesis.

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http://hdl.handle.net/10453/174511