Differences in the transcriptional immune response to Albugo candida between white rust resistant and susceptible cultivars in Brassica rapa L.

Miyaji, N; Akter, MA; Shimizu, M; Mehraj, H; Doullah, MA-U; Dennis, ES; Chuma, I; Fujimoto, R

Differences in the transcriptional immune response to Albugo candida between white rust resistant and susceptible cultivars in Brassica rapa L.

Miyaji, N Akter, MA Shimizu, M Mehraj, H Doullah, MA-U Dennis, ES Chuma, I Fujimoto, R

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Publisher:: NATURE PORTFOLIO
Publication Type:: Journal Article
Citation:: Sci Rep, 2023, 13, (1), pp. 8599
Issue Date:: 2023-05-26

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Field	Value	Language
dc.contributor.author	Miyaji, N
dc.contributor.author	Akter, MA
dc.contributor.author	Shimizu, M
dc.contributor.author	Mehraj, H
dc.contributor.author	Doullah, MA-U
dc.contributor.author	Dennis, ES
dc.contributor.author	Chuma, I
dc.contributor.author	Fujimoto, R
dc.date.accessioned	2024-01-16T00:50:39Z
dc.date.available	2023-05-14
dc.date.available	2024-01-16T00:50:39Z
dc.date.issued	2023-05-26
dc.identifier.citation	Sci Rep, 2023, 13, (1), pp. 8599
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/174528
dc.description.abstract	Albugo candida causing white rust disease decreases the yield of Brassica rapa vegetables greatly. Resistant and susceptible cultivars in B. rapa vegetables have different immune responses against A. candida inoculation, however, the mechanism of how host plants respond to A. candida is still unknown. Using RNA-sequencing, we identified differentially expressed genes (DEGs) between A. candida inoculated [48 and 72 h after inoculation (HAI)] and non-inoculated samples in resistant and susceptible cultivars of komatsuna (B. rapa var. perviridis). Functional DEGs differed between the resistant and susceptible cultivars in A. candida inoculated samples. Salicylic acid (SA) responsive genes tended to be changed in their expression levels by A. candida inoculation in both resistant and susceptible cultivars, but different genes were identified in the two cultivars. SA-dependent systemic acquired resistance (SAR) involving genes were upregulated following A. candida inoculation in the resistant cultivar. Particular genes categorized as SAR that changed expression levels overlapped between A. candida and Fusarium oxysporum f. sp. conglutinans inoculated samples in resistant cultivar, suggesting a role for SAR in defense response to both pathogens particularly in the effector-triggered immunity downstream pathway. These findings will be useful for understanding white rust resistance mechanisms in B. rapa.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE PORTFOLIO
dc.relation.ispartof	Sci Rep
dc.relation.isbasedon	10.1038/s41598-023-35205-5
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Brassica rapa
dc.subject.mesh	Disease Susceptibility
dc.subject.mesh	Immunity, Innate
dc.subject.mesh	Oomycetes
dc.subject.mesh	Plant Diseases
dc.subject.mesh	Oomycetes
dc.subject.mesh	Brassica rapa
dc.subject.mesh	Disease Susceptibility
dc.subject.mesh	Plant Diseases
dc.subject.mesh	Immunity, Innate
dc.subject.mesh	Brassica rapa
dc.subject.mesh	Disease Susceptibility
dc.subject.mesh	Immunity, Innate
dc.subject.mesh	Oomycetes
dc.subject.mesh	Plant Diseases
dc.title	Differences in the transcriptional immune response to Albugo candida between white rust resistant and susceptible cultivars in Brassica rapa L.
dc.type	Journal Article
utslib.citation.volume	13
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2024-01-16T00:50:35Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	13
utslib.citation.issue	1

Abstract:

Albugo candida causing white rust disease decreases the yield of Brassica rapa vegetables greatly. Resistant and susceptible cultivars in B. rapa vegetables have different immune responses against A. candida inoculation, however, the mechanism of how host plants respond to A. candida is still unknown. Using RNA-sequencing, we identified differentially expressed genes (DEGs) between A. candida inoculated [48 and 72 h after inoculation (HAI)] and non-inoculated samples in resistant and susceptible cultivars of komatsuna (B. rapa var. perviridis). Functional DEGs differed between the resistant and susceptible cultivars in A. candida inoculated samples. Salicylic acid (SA) responsive genes tended to be changed in their expression levels by A. candida inoculation in both resistant and susceptible cultivars, but different genes were identified in the two cultivars. SA-dependent systemic acquired resistance (SAR) involving genes were upregulated following A. candida inoculation in the resistant cultivar. Particular genes categorized as SAR that changed expression levels overlapped between A. candida and Fusarium oxysporum f. sp. conglutinans inoculated samples in resistant cultivar, suggesting a role for SAR in defense response to both pathogens particularly in the effector-triggered immunity downstream pathway. These findings will be useful for understanding white rust resistance mechanisms in B. rapa.

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http://hdl.handle.net/10453/174528