Responses of retinal and brain microvasculature to streptozotocin induced diabetes revealed by global expression profiling.

Li, Y; Faiz, A; Moshage, H; Schilling, L; Kamps, JA

Responses of retinal and brain microvasculature to streptozotocin induced diabetes revealed by global expression profiling.

Li, Y Faiz, A

Moshage, H Schilling, L Kamps, JA

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Publisher:: SAGE PUBLICATIONS LTD
Publication Type:: Journal Article
Citation:: Diab Vasc Dis Res, 2023, 20, (1), pp. 14791641221147533
Issue Date:: 2023

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Field	Value	Language
dc.contributor.author	Li, Y
dc.contributor.author	Faiz, A https://orcid.org/0000-0003-1740-3538
dc.contributor.author	Moshage, H
dc.contributor.author	Schilling, L
dc.contributor.author	Kamps, JA
dc.date.accessioned	2024-01-16T06:15:18Z
dc.date.available	2024-01-16T06:15:18Z
dc.date.issued	2023
dc.identifier.citation	Diab Vasc Dis Res, 2023, 20, (1), pp. 14791641221147533
dc.identifier.issn	1479-1641
dc.identifier.issn	1752-8984
dc.identifier.uri	http://hdl.handle.net/10453/174604
dc.description.abstract	This study aims to determine the effects of diabetes in the retinal and brain microvasculature through gene expression profiling. Twelve male Wistar rats were randomly divided into two groups: streptozotocin-induced diabetic rats and time-matched nondiabetic rats. The retinal microvessels (RMVs) and brain microvessels (BMVs) were mechanically isolated from individual rats. Differentially expressed genes (DEGs) in diabetic and nondiabetic microvessels were identified by cDNA microarrays analysis. In RMVs, we identified 43 DEGs, of which 20 were upregulated while 23 were downregulated by diabetes. In BMVs, 35 genes DEGs were identified, of which 22 were upregulated and 13 were downregulated by diabetes. Altered expression of the Nars, Gars, Mars, Iars, Yars, Bcl2, Nqo1, NR4A3, Gpd1, Stc1, Tsc22d3, Tnfrsf21 mRNA as observed in the microarray analyses, was confirmed by quantitative RT-PCR. The aminoacyl-tRNA synthetases (aaRSs) pathway in RMVs was significantly overrepresented as compared to BMVs. Our study demonstrates for the first time that in the brain microvasculature multiple compensatory mechanisms exists, serving to protect brain tissue from diabetic insults, whereas these mechanisms are not activated in the retinal microvasculature. This provides new insights as to why brain microvasculature is less susceptible to diabetes.
dc.format	Print
dc.language	eng
dc.publisher	SAGE PUBLICATIONS LTD
dc.relation.ispartof	Diab Vasc Dis Res
dc.relation.isbasedon	10.1177/14791641221147533
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1103 Clinical Sciences, 1115 Pharmacology and Pharmaceutical Sciences, 1116 Medical Physiology
dc.subject.classification	Endocrinology & Metabolism
dc.subject.classification	3202 Clinical sciences
dc.subject.mesh	Animals
dc.subject.mesh	Male
dc.subject.mesh	Rats
dc.subject.mesh	Brain
dc.subject.mesh	Diabetes Mellitus, Experimental
dc.subject.mesh	Diabetic Retinopathy
dc.subject.mesh	Microvessels
dc.subject.mesh	Rats, Wistar
dc.subject.mesh	Retinal Vessels
dc.subject.mesh	Streptozocin
dc.subject.mesh	Retinal Vessels
dc.subject.mesh	Brain
dc.subject.mesh	Animals
dc.subject.mesh	Rats
dc.subject.mesh	Rats, Wistar
dc.subject.mesh	Diabetic Retinopathy
dc.subject.mesh	Diabetes Mellitus, Experimental
dc.subject.mesh	Streptozocin
dc.subject.mesh	Male
dc.subject.mesh	Microvessels
dc.subject.mesh	Animals
dc.subject.mesh	Male
dc.subject.mesh	Rats
dc.subject.mesh	Brain
dc.subject.mesh	Diabetes Mellitus, Experimental
dc.subject.mesh	Diabetic Retinopathy
dc.subject.mesh	Microvessels
dc.subject.mesh	Rats, Wistar
dc.subject.mesh	Retinal Vessels
dc.subject.mesh	Streptozocin
dc.title	Responses of retinal and brain microvasculature to streptozotocin induced diabetes revealed by global expression profiling.
dc.type	Journal Article
utslib.citation.volume	20
utslib.location.activity	England
utslib.for	1103 Clinical Sciences
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
utslib.for	1116 Medical Physiology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
pubs.organisational-group	/University of Technology Sydney/Strength - CFI - Centre for Inflammation
utslib.copyright.status	open_access	*
dc.date.updated	2024-01-16T06:15:14Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	20
utslib.citation.issue	1

Abstract:

This study aims to determine the effects of diabetes in the retinal and brain microvasculature through gene expression profiling. Twelve male Wistar rats were randomly divided into two groups: streptozotocin-induced diabetic rats and time-matched nondiabetic rats. The retinal microvessels (RMVs) and brain microvessels (BMVs) were mechanically isolated from individual rats. Differentially expressed genes (DEGs) in diabetic and nondiabetic microvessels were identified by cDNA microarrays analysis. In RMVs, we identified 43 DEGs, of which 20 were upregulated while 23 were downregulated by diabetes. In BMVs, 35 genes DEGs were identified, of which 22 were upregulated and 13 were downregulated by diabetes. Altered expression of the Nars, Gars, Mars, Iars, Yars, Bcl2, Nqo1, NR4A3, Gpd1, Stc1, Tsc22d3, Tnfrsf21 mRNA as observed in the microarray analyses, was confirmed by quantitative RT-PCR. The aminoacyl-tRNA synthetases (aaRSs) pathway in RMVs was significantly overrepresented as compared to BMVs. Our study demonstrates for the first time that in the brain microvasculature multiple compensatory mechanisms exists, serving to protect brain tissue from diabetic insults, whereas these mechanisms are not activated in the retinal microvasculature. This provides new insights as to why brain microvasculature is less susceptible to diabetes.

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http://hdl.handle.net/10453/174604