Quantitative Point of Care Tests for Timely Diagnosis of Early-Onset Preeclampsia with High Sensitivity and Specificity.

Masoumeh Ghorbanpour, S; Wen, S; Kaitu'u-Lino, TJ; Hannan, NJ; Jin, D; McClements, L

Quantitative Point of Care Tests for Timely Diagnosis of Early-Onset Preeclampsia with High Sensitivity and Specificity.

Masoumeh Ghorbanpour, S Wen, S

Kaitu'u-Lino, TJ Hannan, NJ Jin, D

McClements, L

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Publisher:: WILEY-V C H VERLAG GMBH
Publication Type:: Journal Article
Citation:: Angew Chem Int Ed Engl, 2023, 62, (26), pp. e202301193
Issue Date:: 2023-06-26

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Field	Value	Language
dc.contributor.author	Masoumeh Ghorbanpour, S
dc.contributor.author	Wen, S https://orcid.org/0000-0002-4670-4658
dc.contributor.author	Kaitu'u-Lino, TJ
dc.contributor.author	Hannan, NJ
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.contributor.author	McClements, L https://orcid.org/0000-0002-4911-1014
dc.date.accessioned	2024-01-17T06:41:57Z
dc.date.available	2024-01-17T06:41:57Z
dc.date.issued	2023-06-26
dc.identifier.citation	Angew Chem Int Ed Engl, 2023, 62, (26), pp. e202301193
dc.identifier.issn	1433-7851
dc.identifier.issn	1521-3773
dc.identifier.uri	http://hdl.handle.net/10453/174721
dc.description.abstract	Preeclampsia is a heterogeneous and multiorgan cardiovascular disorder of pregnancy. Here, we report the development of a novel strip-based lateral flow assay (LFA) using lanthanide-doped upconversion nanoparticles conjugated to antibodies targeting two different biomarkers for detection of preeclampsia. We first measured circulating plasma FKBPL and CD44 protein concentrations from individuals with early-onset preeclampsia (EOPE), using ELISA. We confirmed that the CD44/FKBPL ratio is reduced in EOPE with a good diagnostic potential. Using our rapid LFA prototypes, we achieved an improved lower limit of detection: 10 pg ml-1 for FKBPL and 15 pg ml-1 for CD44, which is more than one order lower than the standard ELISA method. Using clinical samples, a cut-off value of 1.24 for CD44/FKBPL ratio provided positive predictive value of 100 % and the negative predictive value of 91 %. Our LFA shows promise as a rapid and highly sensitive point-of-care test for preeclampsia.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY-V C H VERLAG GMBH
dc.relation	http://purl.org/au-research/grants/arc/DE220100846
dc.relation	http://purl.org/au-research/grants/arc/IH150100028
dc.relation	http://purl.org/au-research/grants/arc/FL210100180
dc.relation.ispartof	Angew Chem Int Ed Engl
dc.relation.isbasedon	10.1002/anie.202301193
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences
dc.subject.classification	Organic Chemistry
dc.subject.classification	34 Chemical sciences
dc.subject.mesh	Pregnancy
dc.subject.mesh	Female
dc.subject.mesh	Humans
dc.subject.mesh	Pre-Eclampsia
dc.subject.mesh	Sensitivity and Specificity
dc.subject.mesh	Point-of-Care Testing
dc.subject.mesh	Biomarkers
dc.subject.mesh	Tacrolimus Binding Proteins
dc.subject.mesh	Humans
dc.subject.mesh	Pre-Eclampsia
dc.subject.mesh	Tacrolimus Binding Proteins
dc.subject.mesh	Sensitivity and Specificity
dc.subject.mesh	Pregnancy
dc.subject.mesh	Female
dc.subject.mesh	Biomarkers
dc.subject.mesh	Point-of-Care Testing
dc.subject.mesh	Pregnancy
dc.subject.mesh	Female
dc.subject.mesh	Humans
dc.subject.mesh	Pre-Eclampsia
dc.subject.mesh	Sensitivity and Specificity
dc.subject.mesh	Point-of-Care Testing
dc.subject.mesh	Biomarkers
dc.subject.mesh	Tacrolimus Binding Proteins
dc.title	Quantitative Point of Care Tests for Timely Diagnosis of Early-Onset Preeclampsia with High Sensitivity and Specificity.
dc.type	Journal Article
utslib.citation.volume	62
utslib.location.activity	Germany
utslib.for	03 Chemical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access	*
dc.date.updated	2024-01-17T06:41:55Z
pubs.issue	26
pubs.publication-status	Published
pubs.volume	62
utslib.citation.issue	26

Abstract:

Preeclampsia is a heterogeneous and multiorgan cardiovascular disorder of pregnancy. Here, we report the development of a novel strip-based lateral flow assay (LFA) using lanthanide-doped upconversion nanoparticles conjugated to antibodies targeting two different biomarkers for detection of preeclampsia. We first measured circulating plasma FKBPL and CD44 protein concentrations from individuals with early-onset preeclampsia (EOPE), using ELISA. We confirmed that the CD44/FKBPL ratio is reduced in EOPE with a good diagnostic potential. Using our rapid LFA prototypes, we achieved an improved lower limit of detection: 10 pg ml-1 for FKBPL and 15 pg ml-1 for CD44, which is more than one order lower than the standard ELISA method. Using clinical samples, a cut-off value of 1.24 for CD44/FKBPL ratio provided positive predictive value of 100 % and the negative predictive value of 91 %. Our LFA shows promise as a rapid and highly sensitive point-of-care test for preeclampsia.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/174721