Quantitative Point of Care Tests for Timely Diagnosis of Early-Onset Preeclampsia with High Sensitivity and Specificity.
- Publisher:
- WILEY-V C H VERLAG GMBH
- Publication Type:
- Journal Article
- Citation:
- Angew Chem Int Ed Engl, 2023, 62, (26), pp. e202301193
- Issue Date:
- 2023-06-26
Closed Access
Filename | Description | Size | |||
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Angew Chem Int Ed - 2023 - Masoumeh Ghorbanpour - Quantitative Point of Care Tests for Timely Diagnosis of Early‐Onset.pdf | Published version | 1.95 MB | Adobe PDF |
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Masoumeh Ghorbanpour, S | |
dc.contributor.author |
Wen, S https://orcid.org/0000-0002-4670-4658 |
|
dc.contributor.author | Kaitu'u-Lino, TJ | |
dc.contributor.author | Hannan, NJ | |
dc.contributor.author |
Jin, D https://orcid.org/0000-0003-1046-2666 |
|
dc.contributor.author |
McClements, L https://orcid.org/0000-0002-4911-1014 |
|
dc.date.accessioned | 2024-01-17T06:41:57Z | |
dc.date.available | 2024-01-17T06:41:57Z | |
dc.date.issued | 2023-06-26 | |
dc.identifier.citation | Angew Chem Int Ed Engl, 2023, 62, (26), pp. e202301193 | |
dc.identifier.issn | 1433-7851 | |
dc.identifier.issn | 1521-3773 | |
dc.identifier.uri | http://hdl.handle.net/10453/174721 | |
dc.description.abstract | Preeclampsia is a heterogeneous and multiorgan cardiovascular disorder of pregnancy. Here, we report the development of a novel strip-based lateral flow assay (LFA) using lanthanide-doped upconversion nanoparticles conjugated to antibodies targeting two different biomarkers for detection of preeclampsia. We first measured circulating plasma FKBPL and CD44 protein concentrations from individuals with early-onset preeclampsia (EOPE), using ELISA. We confirmed that the CD44/FKBPL ratio is reduced in EOPE with a good diagnostic potential. Using our rapid LFA prototypes, we achieved an improved lower limit of detection: 10 pg ml-1 for FKBPL and 15 pg ml-1 for CD44, which is more than one order lower than the standard ELISA method. Using clinical samples, a cut-off value of 1.24 for CD44/FKBPL ratio provided positive predictive value of 100 % and the negative predictive value of 91 %. Our LFA shows promise as a rapid and highly sensitive point-of-care test for preeclampsia. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | WILEY-V C H VERLAG GMBH | |
dc.relation | http://purl.org/au-research/grants/arc/DE220100846 | |
dc.relation | http://purl.org/au-research/grants/arc/IH150100028 | |
dc.relation | http://purl.org/au-research/grants/arc/FL210100180 | |
dc.relation.ispartof | Angew Chem Int Ed Engl | |
dc.relation.isbasedon | 10.1002/anie.202301193 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | 03 Chemical Sciences | |
dc.subject.classification | Organic Chemistry | |
dc.subject.classification | 34 Chemical sciences | |
dc.subject.mesh | Pregnancy | |
dc.subject.mesh | Female | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Pre-Eclampsia | |
dc.subject.mesh | Sensitivity and Specificity | |
dc.subject.mesh | Point-of-Care Testing | |
dc.subject.mesh | Biomarkers | |
dc.subject.mesh | Tacrolimus Binding Proteins | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Pre-Eclampsia | |
dc.subject.mesh | Tacrolimus Binding Proteins | |
dc.subject.mesh | Sensitivity and Specificity | |
dc.subject.mesh | Pregnancy | |
dc.subject.mesh | Female | |
dc.subject.mesh | Biomarkers | |
dc.subject.mesh | Point-of-Care Testing | |
dc.subject.mesh | Pregnancy | |
dc.subject.mesh | Female | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Pre-Eclampsia | |
dc.subject.mesh | Sensitivity and Specificity | |
dc.subject.mesh | Point-of-Care Testing | |
dc.subject.mesh | Biomarkers | |
dc.subject.mesh | Tacrolimus Binding Proteins | |
dc.title | Quantitative Point of Care Tests for Timely Diagnosis of Early-Onset Preeclampsia with High Sensitivity and Specificity. | |
dc.type | Journal Article | |
utslib.citation.volume | 62 | |
utslib.location.activity | Germany | |
utslib.for | 03 Chemical Sciences | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Life Sciences | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences | |
pubs.organisational-group | /University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices | |
utslib.copyright.status | closed_access | * |
dc.date.updated | 2024-01-17T06:41:55Z | |
pubs.issue | 26 | |
pubs.publication-status | Published | |
pubs.volume | 62 | |
utslib.citation.issue | 26 |
Abstract:
Preeclampsia is a heterogeneous and multiorgan cardiovascular disorder of pregnancy. Here, we report the development of a novel strip-based lateral flow assay (LFA) using lanthanide-doped upconversion nanoparticles conjugated to antibodies targeting two different biomarkers for detection of preeclampsia. We first measured circulating plasma FKBPL and CD44 protein concentrations from individuals with early-onset preeclampsia (EOPE), using ELISA. We confirmed that the CD44/FKBPL ratio is reduced in EOPE with a good diagnostic potential. Using our rapid LFA prototypes, we achieved an improved lower limit of detection: 10 pg ml-1 for FKBPL and 15 pg ml-1 for CD44, which is more than one order lower than the standard ELISA method. Using clinical samples, a cut-off value of 1.24 for CD44/FKBPL ratio provided positive predictive value of 100 % and the negative predictive value of 91 %. Our LFA shows promise as a rapid and highly sensitive point-of-care test for preeclampsia.
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