Neurogenic marker expression in differentiating human adipose derived adult mesenchymal stem cells.

Pelegri, NG; Milthorpe, BK; Gorrie, CA; Santos, J

Neurogenic marker expression in differentiating human adipose derived adult mesenchymal stem cells.

Pelegri, NG Milthorpe, BK Gorrie, CA Santos, J

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Publisher:: AME Publishing Company
Publication Type:: Journal Article
Citation:: Stem Cell Investig, 2023, 10, (7), pp. 7
Issue Date:: 2023

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Field	Value	Language
dc.contributor.author	Pelegri, NG
dc.contributor.author	Milthorpe, BK
dc.contributor.author	Gorrie, CA
dc.contributor.author	Santos, J https://orcid.org/0000-0003-4067-220X
dc.date.accessioned	2024-01-17T06:57:21Z
dc.date.available	2023-02-16
dc.date.available	2024-01-17T06:57:21Z
dc.date.issued	2023
dc.identifier.citation	Stem Cell Investig, 2023, 10, (7), pp. 7
dc.identifier.issn	2306-9759
dc.identifier.issn	2313-0792
dc.identifier.uri	http://hdl.handle.net/10453/174725
dc.description.abstract	BACKGROUND: Adipose-derived stem cells (ADSCs) are increasingly utilised in the field of neural regeneration due to their high accessibility and capacity for differentiation into neural like cells. Culturing ADSCs in the presence of various growth factors, small molecules and combinations thereof have shown promise in this regard; however, these protocols are generally complex, time-consuming and costly. The need for commercially available and chemically defined growth media/supplements is required to facilitate further developments in this area. METHODS: In this study, we have examined the neural differentiation and proliferation potential of the commercially available supplements B27, CultureOne (C1) and N2 on human ADSCs (hADSCs). Through a combination of immunocytochemistry, cytokine analysis, and CNPase enzymatic assays, we provide novel insight into the neural differentiation effects of B27, C1 and N2 on hADSCs. RESULTS: The study found that C1 and N2 supplements initiated neural differentiation of the cells, with C1 pushing differentiation towards an oligodendrocytic lineage and N2 initiating neuronal differentiation. This suggests that C1 and N2 supplements can be used to drive neural differentiation in hADSCs. However, B27 did not show significant differentiation in the time frame in which the experiments took place and therefore is unsuitable for this purpose. CONCLUSIONS: These findings highlight the utility of commercially available supplements in the neural differentiation of ADSCs and may assist in establishing simpler, more affordable differentiation protocols.
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	AME Publishing Company
dc.relation.ispartof	Stem Cell Investig
dc.relation.isbasedon	10.21037/sci-2022-015
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Neurogenic marker expression in differentiating human adipose derived adult mesenchymal stem cells.
dc.type	Journal Article
utslib.citation.volume	10
utslib.location.activity	China
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2024-01-17T06:57:10Z
pubs.issue	7
pubs.publication-status	Published online
pubs.volume	10
utslib.citation.issue	7

Abstract:

BACKGROUND: Adipose-derived stem cells (ADSCs) are increasingly utilised in the field of neural regeneration due to their high accessibility and capacity for differentiation into neural like cells. Culturing ADSCs in the presence of various growth factors, small molecules and combinations thereof have shown promise in this regard; however, these protocols are generally complex, time-consuming and costly. The need for commercially available and chemically defined growth media/supplements is required to facilitate further developments in this area. METHODS: In this study, we have examined the neural differentiation and proliferation potential of the commercially available supplements B27, CultureOne (C1) and N2 on human ADSCs (hADSCs). Through a combination of immunocytochemistry, cytokine analysis, and CNPase enzymatic assays, we provide novel insight into the neural differentiation effects of B27, C1 and N2 on hADSCs. RESULTS: The study found that C1 and N2 supplements initiated neural differentiation of the cells, with C1 pushing differentiation towards an oligodendrocytic lineage and N2 initiating neuronal differentiation. This suggests that C1 and N2 supplements can be used to drive neural differentiation in hADSCs. However, B27 did not show significant differentiation in the time frame in which the experiments took place and therefore is unsuitable for this purpose. CONCLUSIONS: These findings highlight the utility of commercially available supplements in the neural differentiation of ADSCs and may assist in establishing simpler, more affordable differentiation protocols.

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http://hdl.handle.net/10453/174725