Native Function of the Bacterial Ion Channel SthK in a Sparsely Tethered Lipid Bilayer Membrane Architecture.

Andersson, J; Kleinheinz, D; Ramach, U; Kiesenhofer, N; Ashenden, A; Valtiner, M; Holt, S; Koeper, I; Schmidpeter, PAM; Knoll, W

Native Function of the Bacterial Ion Channel SthK in a Sparsely Tethered Lipid Bilayer Membrane Architecture.

Andersson, J Kleinheinz, D Ramach, U Kiesenhofer, N Ashenden, A Valtiner, M Holt, S

Koeper, I Schmidpeter, PAM Knoll, W

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: J Phys Chem B, 2023, 127, (16), pp. 3641-3650
Issue Date:: 2023-04-27

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Field	Value	Language
dc.contributor.author	Andersson, J
dc.contributor.author	Kleinheinz, D
dc.contributor.author	Ramach, U
dc.contributor.author	Kiesenhofer, N
dc.contributor.author	Ashenden, A
dc.contributor.author	Valtiner, M
dc.contributor.author	Holt, S https://orcid.org/0000-0003-3189-8047
dc.contributor.author	Koeper, I
dc.contributor.author	Schmidpeter, PAM
dc.contributor.author	Knoll, W
dc.date.accessioned	2024-01-25T06:10:25Z
dc.date.available	2024-01-25T06:10:25Z
dc.date.issued	2023-04-27
dc.identifier.citation	J Phys Chem B, 2023, 127, (16), pp. 3641-3650
dc.identifier.issn	1520-6106
dc.identifier.issn	1520-5207
dc.identifier.uri	http://hdl.handle.net/10453/174957
dc.description.abstract	The plasma membrane protects the interiors of cells from their surroundings and also plays a critical role in communication, sensing, and nutrient import. As a result, the cell membrane and its constituents are among the most important drug targets. Studying the cell membrane and the processes it facilitates is therefore crucial, but it is a highly complex environment that is difficult to access experimentally. Various model membrane systems have been developed to provide an environment in which membrane proteins can be studied in isolation. Among them, tethered bilayer lipid membranes (tBLMs) are a promising model system providing a solvent-free membrane environment which can be prepared by self-assembly, is resistant to mechanical disturbances and has a high electrical resistance. tBLMs are therefore uniquely suitable to study ion channels and charge transport processes. However, ion channels are often large, complex, multimeric structures and their function requires a particular lipid environment. In this paper, we show that SthK, a bacterial cyclic nucleotide gated (CNG) ion channel that is strongly dependent on the surrounding lipid composition, functions normally when embedded into a sparsely tethered lipid bilayer. As SthK has been very well characterized in terms of structure and function, it is well-suited to demonstrate the utility of tethered membrane systems. A model membrane system suitable for studying CNG ion channels would be useful, as this type of ion channel performs a wide range of physiological functions in bacteria, plants, and mammals and is therefore of fundamental scientific interest as well as being highly relevant to medicine.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation.ispartof	J Phys Chem B
dc.relation.isbasedon	10.1021/acs.jpcb.2c07252
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	02 Physical Sciences, 03 Chemical Sciences, 09 Engineering
dc.subject.classification	34 Chemical sciences
dc.subject.classification	40 Engineering
dc.subject.classification	51 Physical sciences
dc.subject.mesh	Electrochemical Techniques
dc.subject.mesh	Ion Channels
dc.subject.mesh	Lipid Bilayers
dc.subject.mesh	Microscopy, Atomic Force
dc.subject.mesh	Cyclic AMP
dc.subject.mesh	Bacteria
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Bacteria
dc.subject.mesh	Lipid Bilayers
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Ion Channels
dc.subject.mesh	Cyclic AMP
dc.subject.mesh	Microscopy, Atomic Force
dc.subject.mesh	Electrochemical Techniques
dc.subject.mesh	Electrochemical Techniques
dc.subject.mesh	Ion Channels
dc.subject.mesh	Lipid Bilayers
dc.subject.mesh	Microscopy, Atomic Force
dc.subject.mesh	Cyclic AMP
dc.subject.mesh	Bacteria
dc.subject.mesh	Bacterial Proteins
dc.title	Native Function of the Bacterial Ion Channel SthK in a Sparsely Tethered Lipid Bilayer Membrane Architecture.
dc.type	Journal Article
utslib.citation.volume	127
utslib.location.activity	United States
utslib.for	02 Physical Sciences
utslib.for	03 Chemical Sciences
utslib.for	09 Engineering
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2024-01-25T06:10:18Z
pubs.issue	16
pubs.publication-status	Published
pubs.volume	127
utslib.citation.issue	16

Abstract:

The plasma membrane protects the interiors of cells from their surroundings and also plays a critical role in communication, sensing, and nutrient import. As a result, the cell membrane and its constituents are among the most important drug targets. Studying the cell membrane and the processes it facilitates is therefore crucial, but it is a highly complex environment that is difficult to access experimentally. Various model membrane systems have been developed to provide an environment in which membrane proteins can be studied in isolation. Among them, tethered bilayer lipid membranes (tBLMs) are a promising model system providing a solvent-free membrane environment which can be prepared by self-assembly, is resistant to mechanical disturbances and has a high electrical resistance. tBLMs are therefore uniquely suitable to study ion channels and charge transport processes. However, ion channels are often large, complex, multimeric structures and their function requires a particular lipid environment. In this paper, we show that SthK, a bacterial cyclic nucleotide gated (CNG) ion channel that is strongly dependent on the surrounding lipid composition, functions normally when embedded into a sparsely tethered lipid bilayer. As SthK has been very well characterized in terms of structure and function, it is well-suited to demonstrate the utility of tethered membrane systems. A model membrane system suitable for studying CNG ion channels would be useful, as this type of ion channel performs a wide range of physiological functions in bacteria, plants, and mammals and is therefore of fundamental scientific interest as well as being highly relevant to medicine.

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http://hdl.handle.net/10453/174957