Rapid detection of Escherichia coli in dairy milk using static headspace-comprehensive two-dimensional gas chromatography.
- Publisher:
- SPRINGER HEIDELBERG
- Publication Type:
- Journal Article
- Citation:
- Anal Bioanal Chem, 2023, 415, (13), pp. 2535-2545
- Issue Date:
- 2023-05
Closed Access
Filename | Description | Size | |||
---|---|---|---|---|---|
s00216-022-04485-7 (1).pdf | Published version | 1.24 MB | Adobe PDF |
Copyright Clearance Process
- Recently Added
- In Progress
- Closed Access
This item is closed access and not available.
Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Heng, WS | |
dc.contributor.author | Jadhav, SR | |
dc.contributor.author |
Ueland, M https://orcid.org/0000-0002-9155-3502 |
|
dc.contributor.author | Shellie, RA | |
dc.date.accessioned | 2024-01-31T04:48:23Z | |
dc.date.available | 2022-12-12 | |
dc.date.available | 2024-01-31T04:48:23Z | |
dc.date.issued | 2023-05 | |
dc.identifier.citation | Anal Bioanal Chem, 2023, 415, (13), pp. 2535-2545 | |
dc.identifier.issn | 1618-2642 | |
dc.identifier.issn | 1618-2650 | |
dc.identifier.uri | http://hdl.handle.net/10453/175131 | |
dc.description.abstract | A new approach is introduced for rapid and reliable bacteria detection in food. Namely, static headspace-comprehensive two-dimensional gas chromatography (HS-GC × GC) with backflushing. The introduced approach provides fast detection of Escherichia coli (E. coli) in enriched ultra-high-temperature processed (UHT) dairy milk. The presence of E. coli may be indicated by detecting microbial volatile organic compounds emanating from test solutions inoculated with E. coli. In the present investigation, HS-GC × GC analysis is preceded by conventional enrichment in nutrient broth and inoculated samples are clearly discernable from controls following as little as 15 h sample enrichment. Headspace equilibration for 28 min followed by an 8 min GC × GC analysis of enriched test solutions reduces time-to-response by approximately one full day compared to conventional culture-based methods. The presence of ethanol, 1-propanol, and acetaldehyde may be used as a putative marker of E. coli contamination in milk and the introduced approach is able to detect single-cell initial bacterial load. Faster, reliable detection of pathogens and/or spoilage microbes in food products is desirable for the food industry. The described approach has great potential to complement the conventional workflow and be utilised for rapid microbial screening of foodstuff. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | SPRINGER HEIDELBERG | |
dc.relation | http://purl.org/au-research/grants/arc/DE210100494 | |
dc.relation.ispartof | Anal Bioanal Chem | |
dc.relation.isbasedon | 10.1007/s00216-022-04485-7 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | 03 Chemical Sciences, 06 Biological Sciences, 09 Engineering | |
dc.subject.classification | Analytical Chemistry | |
dc.subject.classification | 31 Biological sciences | |
dc.subject.classification | 34 Chemical sciences | |
dc.subject.classification | 40 Engineering | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Escherichia coli | |
dc.subject.mesh | Gas Chromatography-Mass Spectrometry | |
dc.subject.mesh | Milk | |
dc.subject.mesh | Ethanol | |
dc.subject.mesh | Bacteria | |
dc.subject.mesh | Volatile Organic Compounds | |
dc.subject.mesh | Milk | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Bacteria | |
dc.subject.mesh | Escherichia coli | |
dc.subject.mesh | Ethanol | |
dc.subject.mesh | Gas Chromatography-Mass Spectrometry | |
dc.subject.mesh | Volatile Organic Compounds | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Escherichia coli | |
dc.subject.mesh | Gas Chromatography-Mass Spectrometry | |
dc.subject.mesh | Milk | |
dc.subject.mesh | Ethanol | |
dc.subject.mesh | Bacteria | |
dc.subject.mesh | Volatile Organic Compounds | |
dc.title | Rapid detection of Escherichia coli in dairy milk using static headspace-comprehensive two-dimensional gas chromatography. | |
dc.type | Journal Article | |
utslib.citation.volume | 415 | |
utslib.location.activity | Germany | |
utslib.for | 03 Chemical Sciences | |
utslib.for | 06 Biological Sciences | |
utslib.for | 09 Engineering | |
pubs.organisational-group | University of Technology Sydney | |
pubs.organisational-group | University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | University of Technology Sydney/Strength - CFS - Centre for Forensic Science | |
pubs.organisational-group | University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences | |
utslib.copyright.status | closed_access | * |
dc.date.updated | 2024-01-31T04:48:22Z | |
pubs.issue | 13 | |
pubs.publication-status | Published | |
pubs.volume | 415 | |
utslib.citation.issue | 13 |
Abstract:
A new approach is introduced for rapid and reliable bacteria detection in food. Namely, static headspace-comprehensive two-dimensional gas chromatography (HS-GC × GC) with backflushing. The introduced approach provides fast detection of Escherichia coli (E. coli) in enriched ultra-high-temperature processed (UHT) dairy milk. The presence of E. coli may be indicated by detecting microbial volatile organic compounds emanating from test solutions inoculated with E. coli. In the present investigation, HS-GC × GC analysis is preceded by conventional enrichment in nutrient broth and inoculated samples are clearly discernable from controls following as little as 15 h sample enrichment. Headspace equilibration for 28 min followed by an 8 min GC × GC analysis of enriched test solutions reduces time-to-response by approximately one full day compared to conventional culture-based methods. The presence of ethanol, 1-propanol, and acetaldehyde may be used as a putative marker of E. coli contamination in milk and the introduced approach is able to detect single-cell initial bacterial load. Faster, reliable detection of pathogens and/or spoilage microbes in food products is desirable for the food industry. The described approach has great potential to complement the conventional workflow and be utilised for rapid microbial screening of foodstuff.
Please use this identifier to cite or link to this item:
Download statistics for the last 12 months
Not enough data to produce graph