Influenza vaccination features revealed by a single-cell transcriptome atlas.
Wang, Y
Wang, X
Jia, X
Li, J
Fu, J
Huang, X
Cui, X
Wang, B
Luo, W
Lin, C
Li, Z
Luu, LDW
Li, S
Zhu, X
Tai, J
- Publisher:
- WILEY
- Publication Type:
- Journal Article
- Citation:
- J Med Virol, 2023, 95, (1), pp. e28174
- Issue Date:
- 2023-01
Closed Access
Filename | Description | Size | |||
---|---|---|---|---|---|
Journal of Medical Virology - 2022 - Wang - Influenza vaccination features revealed by a single‐cell transcriptome atlas.pdf | Accepted version | 5.68 MB | Adobe PDF |
Copyright Clearance Process
- Recently Added
- In Progress
- Closed Access
This item is closed access and not available.
Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, Y | |
dc.contributor.author | Wang, X | |
dc.contributor.author | Jia, X | |
dc.contributor.author | Li, J | |
dc.contributor.author | Fu, J | |
dc.contributor.author | Huang, X | |
dc.contributor.author | Cui, X | |
dc.contributor.author | Wang, B | |
dc.contributor.author | Luo, W | |
dc.contributor.author | Lin, C | |
dc.contributor.author | Li, Z | |
dc.contributor.author | Luu, LDW | |
dc.contributor.author | Li, S | |
dc.contributor.author | Zhu, X | |
dc.contributor.author | Tai, J | |
dc.date.accessioned | 2024-02-07T07:22:16Z | |
dc.date.available | 2022-09-23 | |
dc.date.available | 2024-02-07T07:22:16Z | |
dc.date.issued | 2023-01 | |
dc.identifier.citation | J Med Virol, 2023, 95, (1), pp. e28174 | |
dc.identifier.issn | 0146-6615 | |
dc.identifier.issn | 1096-9071 | |
dc.identifier.uri | http://hdl.handle.net/10453/175437 | |
dc.description.abstract | Emerging and re-emerging viruses like influenza virus pose a continuous global public health threat. Vaccines are one of the most effective public health strategies for controlling infectious diseases. However, little is known about the immunological features of vaccination at the single-cell resolution, including for influenza vaccination. Here, we report the single-cell transcriptome atlas of longitudinally collected peripheral blood mononuclear cells (PBMCs) in individuals immunized with an inactivated influenza vaccine. Overall, vaccination with the influenza vaccine only had a small impact on the composition of peripheral immune cells, but elicited global transcriptional changes in multiple immune cell subsets. In plasma and B cell subsets, transcriptomic changes, which were mostly involved in antibody production as well as B cell activation and differentiation, were observed after influenza vaccinations. In influenza-vaccinated individuals, we found a reduction in multiple biological processes (e.g., interferon response, inflammatory response, HLA-I/II molecules, cellular apoptosis, migration, and cytotoxicity, etc.,) 7 days postvaccination in multiple immune cell subsets. However, 14 days postvaccination, these levels returned to similar levels observed in prevaccination samples. Additionally, we did not observe significant upregulation of pro-inflammatory response genes and key thrombosis-related genes in influenza-vaccinated individuals. Taken together, we report a cell atlas of the peripheral immune response to influenza vaccination and provide a resource for understanding the immunological response mechanisms of influenza vaccination. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | WILEY | |
dc.relation.ispartof | J Med Virol | |
dc.relation.isbasedon | 10.1002/jmv.28174 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | 0605 Microbiology, 1108 Medical Microbiology | |
dc.subject.classification | Virology | |
dc.subject.classification | 3107 Microbiology | |
dc.subject.classification | 3202 Clinical sciences | |
dc.subject.classification | 3207 Medical microbiology | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Influenza, Human | |
dc.subject.mesh | Influenza Vaccines | |
dc.subject.mesh | Transcriptome | |
dc.subject.mesh | Leukocytes, Mononuclear | |
dc.subject.mesh | Antibodies, Viral | |
dc.subject.mesh | Vaccination | |
dc.subject.mesh | Vaccines, Inactivated | |
dc.subject.mesh | Leukocytes, Mononuclear | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Vaccines, Inactivated | |
dc.subject.mesh | Influenza Vaccines | |
dc.subject.mesh | Antibodies, Viral | |
dc.subject.mesh | Vaccination | |
dc.subject.mesh | Influenza, Human | |
dc.subject.mesh | Transcriptome | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Influenza, Human | |
dc.subject.mesh | Influenza Vaccines | |
dc.subject.mesh | Transcriptome | |
dc.subject.mesh | Leukocytes, Mononuclear | |
dc.subject.mesh | Antibodies, Viral | |
dc.subject.mesh | Vaccination | |
dc.subject.mesh | Vaccines, Inactivated | |
dc.title | Influenza vaccination features revealed by a single-cell transcriptome atlas. | |
dc.type | Journal Article | |
utslib.citation.volume | 95 | |
utslib.location.activity | United States | |
utslib.for | 0605 Microbiology | |
utslib.for | 1108 Medical Microbiology | |
pubs.organisational-group | University of Technology Sydney | |
pubs.organisational-group | University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | University of Technology Sydney/Faculty of Science/School of Life Sciences | |
utslib.copyright.status | closed_access | * |
dc.date.updated | 2024-02-07T07:22:15Z | |
pubs.issue | 1 | |
pubs.publication-status | Published | |
pubs.volume | 95 | |
utslib.citation.issue | 1 |
Abstract:
Emerging and re-emerging viruses like influenza virus pose a continuous global public health threat. Vaccines are one of the most effective public health strategies for controlling infectious diseases. However, little is known about the immunological features of vaccination at the single-cell resolution, including for influenza vaccination. Here, we report the single-cell transcriptome atlas of longitudinally collected peripheral blood mononuclear cells (PBMCs) in individuals immunized with an inactivated influenza vaccine. Overall, vaccination with the influenza vaccine only had a small impact on the composition of peripheral immune cells, but elicited global transcriptional changes in multiple immune cell subsets. In plasma and B cell subsets, transcriptomic changes, which were mostly involved in antibody production as well as B cell activation and differentiation, were observed after influenza vaccinations. In influenza-vaccinated individuals, we found a reduction in multiple biological processes (e.g., interferon response, inflammatory response, HLA-I/II molecules, cellular apoptosis, migration, and cytotoxicity, etc.,) 7 days postvaccination in multiple immune cell subsets. However, 14 days postvaccination, these levels returned to similar levels observed in prevaccination samples. Additionally, we did not observe significant upregulation of pro-inflammatory response genes and key thrombosis-related genes in influenza-vaccinated individuals. Taken together, we report a cell atlas of the peripheral immune response to influenza vaccination and provide a resource for understanding the immunological response mechanisms of influenza vaccination.
Please use this identifier to cite or link to this item:
Download statistics for the last 12 months
Not enough data to produce graph