Population Control of Upconversion Energy Transfer for Stimulation Emission Depletion Nanoscopy.

Liu, Y; Wen, S; Wang, F; Zuo, C; Chen, C; Zhou, J; Jin, D

Population Control of Upconversion Energy Transfer for Stimulation Emission Depletion Nanoscopy.

Liu, Y Wen, S

Wang, F Zuo, C Chen, C

Zhou, J

Jin, D

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: Adv Sci (Weinh), 2023, 10, (20), pp. e2205990
Issue Date:: 2023-07

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Field	Value	Language
dc.contributor.author	Liu, Y
dc.contributor.author	Wen, S https://orcid.org/0000-0002-4670-4658
dc.contributor.author	Wang, F
dc.contributor.author	Zuo, C
dc.contributor.author	Chen, C https://orcid.org/0000-0003-4620-7771
dc.contributor.author	Zhou, J https://orcid.org/0000-0002-0605-5745
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.date.accessioned	2024-02-08T23:58:55Z
dc.date.available	2024-02-08T23:58:55Z
dc.date.issued	2023-07
dc.identifier.citation	Adv Sci (Weinh), 2023, 10, (20), pp. e2205990
dc.identifier.issn	2198-3844
dc.identifier.issn	2198-3844
dc.identifier.uri	http://hdl.handle.net/10453/175527
dc.description.abstract	Upconverting stimulated emission depletion microscopy (U-STED) is emerging as an effective approach for super-resolution imaging due to its significantly low depletion power and its ability to surpass the limitations of the square-root law and achieve higher resolution. Though the compelling performance, a trade-off between the spatial resolution and imaging quality in U-STED has been recognized in restricting the usability due to the low excitation power drove high depletion efficiency. Moreover, it is a burden to search for the right power relying on trial and error as the underpinning mechanism is unknown. Here, a method is proposed that can easily predict the ideal excitation power for high depletion efficiency with the assistance of the non-saturate excitation based on the dynamic cross-relaxation (CR) energy transfer of upconversion nanoparticles. This allows the authors to employ the rate equation model to simulate the populations of each relevant energy state of lanthanides and predict the ideal excitation power for high depletion efficiency. The authors demonstrate that the resolution of STED with the assistance of nonsaturated confocal super-resolution results can easily achieve the highest resolution of sub-40 nm, 1/24th of the excitation wavelengths. The finding on the CR effect provides opportunities for population control in realizing low-power high-resolution nanoscopy.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY
dc.relation	http://purl.org/au-research/grants/arc/FL210100180
dc.relation	http://purl.org/au-research/grants/arc/FT220100018
dc.relation.ispartof	Adv Sci (Weinh)
dc.relation.isbasedon	10.1002/advs.202205990
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Population Control of Upconversion Energy Transfer for Stimulation Emission Depletion Nanoscopy.
dc.type	Journal Article
utslib.citation.volume	10
utslib.location.activity	Germany
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access	*
dc.date.updated	2024-02-08T23:58:54Z
pubs.issue	20
pubs.publication-status	Published
pubs.volume	10
utslib.citation.issue	20

Abstract:

Upconverting stimulated emission depletion microscopy (U-STED) is emerging as an effective approach for super-resolution imaging due to its significantly low depletion power and its ability to surpass the limitations of the square-root law and achieve higher resolution. Though the compelling performance, a trade-off between the spatial resolution and imaging quality in U-STED has been recognized in restricting the usability due to the low excitation power drove high depletion efficiency. Moreover, it is a burden to search for the right power relying on trial and error as the underpinning mechanism is unknown. Here, a method is proposed that can easily predict the ideal excitation power for high depletion efficiency with the assistance of the non-saturate excitation based on the dynamic cross-relaxation (CR) energy transfer of upconversion nanoparticles. This allows the authors to employ the rate equation model to simulate the populations of each relevant energy state of lanthanides and predict the ideal excitation power for high depletion efficiency. The authors demonstrate that the resolution of STED with the assistance of nonsaturated confocal super-resolution results can easily achieve the highest resolution of sub-40 nm, 1/24th of the excitation wavelengths. The finding on the CR effect provides opportunities for population control in realizing low-power high-resolution nanoscopy.

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http://hdl.handle.net/10453/175527