Characterizing isoprene production in cyanobacteria - Insights into the effects of light, temperature, and isoprene on Synechocystis sp. PCC 6803.

Rodrigues, JS; Kovács, L; Lukeš, M; Höper, R; Steuer, R; Červený, J; Lindberg, P; Zavřel, T

Characterizing isoprene production in cyanobacteria - Insights into the effects of light, temperature, and isoprene on Synechocystis sp. PCC 6803.

Rodrigues, JS Kovács, L Lukeš, M Höper, R Steuer, R Červený, J Lindberg, P Zavřel, T

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Publisher:: ELSEVIER SCI LTD
Publication Type:: Journal Article
Citation:: Bioresour Technol, 2023, 380, pp. 129068
Issue Date:: 2023-07

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Field	Value	Language
dc.contributor.author	Rodrigues, JS
dc.contributor.author	Kovács, L
dc.contributor.author	Lukeš, M
dc.contributor.author	Höper, R
dc.contributor.author	Steuer, R
dc.contributor.author	Červený, J
dc.contributor.author	Lindberg, P
dc.contributor.author	Zavřel, T
dc.date.accessioned	2024-02-09T03:11:58Z
dc.date.available	2023-04-16
dc.date.available	2024-02-09T03:11:58Z
dc.date.issued	2023-07
dc.identifier.citation	Bioresour Technol, 2023, 380, pp. 129068
dc.identifier.issn	0960-8524
dc.identifier.issn	1873-2976
dc.identifier.uri	http://hdl.handle.net/10453/175545
dc.description.abstract	Engineering cyanobacteria for the production of isoprene and other terpenoids has gained increasing attention in the field of biotechnology. Several studies have addressed optimization of isoprene synthesis in cyanobacteria via enzyme and pathway engineering. However, only little attention has been paid to the optimization of cultivation conditions. In this study, an isoprene-producing strain of Synechocystis sp. PCC 6803 and two control strains were grown under a variety of cultivation conditions. Isoprene production, as quantified by modified membrane inlet mass spectrometer (MIMS) and interpreted using Flux Balance Analysis (FBA), increased under violet light and at elevated temperature. Increase of thermotolerance in the isoprene producer was attributed to the physical presence of isoprene, similar to plants. The results demonstrate a beneficial effect of isoprene on cell survival at higher temperatures. This increased thermotolerance opens new possibilities for sustainable bio-production of isoprene and other products.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER SCI LTD
dc.relation.ispartof	Bioresour Technol
dc.relation.isbasedon	10.1016/j.biortech.2023.129068
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Biotechnology
dc.subject.classification	3001 Agricultural biotechnology
dc.subject.classification	3106 Industrial biotechnology
dc.subject.classification	3107 Microbiology
dc.subject.mesh	Synechocystis
dc.subject.mesh	Temperature
dc.subject.mesh	Hemiterpenes
dc.subject.mesh	Butadienes
dc.subject.mesh	Synechocystis
dc.subject.mesh	Butadienes
dc.subject.mesh	Hemiterpenes
dc.subject.mesh	Temperature
dc.title	Characterizing isoprene production in cyanobacteria - Insights into the effects of light, temperature, and isoprene on Synechocystis sp. PCC 6803.
dc.type	Journal Article
utslib.citation.volume	380
utslib.location.activity	England
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access	*
dc.date.updated	2024-02-09T03:11:57Z
pubs.publication-status	Published
pubs.volume	380

Abstract:

Engineering cyanobacteria for the production of isoprene and other terpenoids has gained increasing attention in the field of biotechnology. Several studies have addressed optimization of isoprene synthesis in cyanobacteria via enzyme and pathway engineering. However, only little attention has been paid to the optimization of cultivation conditions. In this study, an isoprene-producing strain of Synechocystis sp. PCC 6803 and two control strains were grown under a variety of cultivation conditions. Isoprene production, as quantified by modified membrane inlet mass spectrometer (MIMS) and interpreted using Flux Balance Analysis (FBA), increased under violet light and at elevated temperature. Increase of thermotolerance in the isoprene producer was attributed to the physical presence of isoprene, similar to plants. The results demonstrate a beneficial effect of isoprene on cell survival at higher temperatures. This increased thermotolerance opens new possibilities for sustainable bio-production of isoprene and other products.

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http://hdl.handle.net/10453/175545