HPLC-MS/MS determination and the postmortem distribution or postmortem redistribution of paraquat and its metabolites in four fatal intoxication cases.

Ma, H; Hu, M; Zhang, C; Jia, J; Fu, S; Wei, Z; Yun, K

HPLC-MS/MS determination and the postmortem distribution or postmortem redistribution of paraquat and its metabolites in four fatal intoxication cases.

Ma, H Hu, M Zhang, C Jia, J Fu, S

Wei, Z Yun, K

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Forensic Sci Int, 2023, 345, pp. 111606
Issue Date:: 2023-04

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Field	Value	Language
dc.contributor.author	Ma, H
dc.contributor.author	Hu, M
dc.contributor.author	Zhang, C
dc.contributor.author	Jia, J
dc.contributor.author	Fu, S https://orcid.org/0000-0002-6238-3612
dc.contributor.author	Wei, Z
dc.contributor.author	Yun, K
dc.date.accessioned	2024-02-09T05:31:38Z
dc.date.available	2023-02-13
dc.date.available	2024-02-09T05:31:38Z
dc.date.issued	2023-04
dc.identifier.citation	Forensic Sci Int, 2023, 345, pp. 111606
dc.identifier.issn	0379-0738
dc.identifier.issn	1872-6283
dc.identifier.uri	http://hdl.handle.net/10453/175550
dc.description.abstract	HPLC-MS/MS analysis and postmortem distribution or postmortem redistribution of paraquat and its two metabolites in poisoning death cases were reported. Paraquat, monoquat, and paraquat monopyridone were extracted from the sample with acetonitrile or methanol, respectively, detected by ZORBAX HILIC Plus (4.6 × 100 mm, 3.5 μm) chromatographic column, with 0.1 % formic acid aqueous solution - 0.1 % formic acid acetonitrile solution (v/v) as mobile phase. Paraquat, monoquat, and paraquat monopyridone had a good linear relationship within the range of 10-1000, 1-400, and 1-1000 ng/mL (or g), the correlation coefficient (r) were all ≥ 0.9996. Their detection limits were lower than 1 ng/mL (or g). The detection accuracy was 91.25∼113.44 %. The intra-day and inter-day precision were 1.51-3.99 % and 1.92-4.93 %, respectively. This method was used to detect and analyze four rare paraquat poisoning cases. The distribution of paraquat, monoquat, and paraquat monopyridone is uneven, which is relatively high in the heart, blood, lung, and kidney. Heart blood/Peripheral blood ratio of paraquat, monoquat, paraquat monopyridone concentration in two poisoned cases were 1.4, 2.0, 1.5 and 1.9, 1.3, 1.2, which showed a location dependent postmortem redistribution. This is the first time that HPLC-MS/MS and the postmortem distribution or postmortem redistribution of paraquat metabolites in poisoned death cases have been reported. This research provides scientific basis for forensic identification of paraquat poisoning cases and extraction of biological specimen.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier
dc.relation.ispartof	Forensic Sci Int
dc.relation.isbasedon	10.1016/j.forsciint.2023.111606
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.subject.classification	Legal & Forensic Medicine
dc.subject.mesh	Paraquat
dc.subject.mesh	Chromatography, High Pressure Liquid
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Formates
dc.subject.mesh	Formates
dc.subject.mesh	Paraquat
dc.subject.mesh	Chromatography, High Pressure Liquid
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Paraquat
dc.subject.mesh	Chromatography, High Pressure Liquid
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Formates
dc.title	HPLC-MS/MS determination and the postmortem distribution or postmortem redistribution of paraquat and its metabolites in four fatal intoxication cases.
dc.type	Journal Article
utslib.citation.volume	345
utslib.location.activity	Ireland
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2024-02-09T05:31:37Z
pubs.publication-status	Published
pubs.volume	345

Abstract:

HPLC-MS/MS analysis and postmortem distribution or postmortem redistribution of paraquat and its two metabolites in poisoning death cases were reported. Paraquat, monoquat, and paraquat monopyridone were extracted from the sample with acetonitrile or methanol, respectively, detected by ZORBAX HILIC Plus (4.6 × 100 mm, 3.5 μm) chromatographic column, with 0.1 % formic acid aqueous solution - 0.1 % formic acid acetonitrile solution (v/v) as mobile phase. Paraquat, monoquat, and paraquat monopyridone had a good linear relationship within the range of 10-1000, 1-400, and 1-1000 ng/mL (or g), the correlation coefficient (r) were all ≥ 0.9996. Their detection limits were lower than 1 ng/mL (or g). The detection accuracy was 91.25∼113.44 %. The intra-day and inter-day precision were 1.51-3.99 % and 1.92-4.93 %, respectively. This method was used to detect and analyze four rare paraquat poisoning cases. The distribution of paraquat, monoquat, and paraquat monopyridone is uneven, which is relatively high in the heart, blood, lung, and kidney. Heart blood/Peripheral blood ratio of paraquat, monoquat, paraquat monopyridone concentration in two poisoned cases were 1.4, 2.0, 1.5 and 1.9, 1.3, 1.2, which showed a location dependent postmortem redistribution. This is the first time that HPLC-MS/MS and the postmortem distribution or postmortem redistribution of paraquat metabolites in poisoned death cases have been reported. This research provides scientific basis for forensic identification of paraquat poisoning cases and extraction of biological specimen.

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http://hdl.handle.net/10453/175550